Check the preprint of our exciting collaboration with @pilarcossio.bsky.social and others: Quantifying RNA Folding Equilibria from Unclassfied Cryo-EM Images biorxiv.org/content/10.6... see Pilar's very nice description. Congrats Deb Maji and team!
Quantifying RNA Folding Equilibria from Unclassified Cryo-EM Particle Images
Functional RNA structures are typically far more dynamic than most proteins solved by cryo-electron microscopy (cryo-EM). Flexible junctions and weak tertiary contacts produce broad conformational ens...
biorxiv.org
@pilarcossio.bsky.social · 2w ago2/5 Our idea: reweight computational RNA ensembles directly against individual, unclassified cryo-EM images, using probabilistic modeling and deep learning. No averaging, so the heterogeneity isn’t thrown away.
