Happy to share the first paper from my postdoc in the Darst and Campbell labs! 🧵 www.pnas.org/doi/full/10.... We used cryo-EM and allosteric inhibitors as mechanistic probes to visualize conformational landscapes of E. coli and M. tuberculosis RNA polymerases during nucleotide addition. 1/4
RNA polymerase inhibitors reveal active-site motions essential for the nucleotide addition cycle | PNAS
The nucleotide addition cycle (NAC) of multisubunit DNA-dependent RNA polymerases (RNAPs) involves coordinated conformational changes in conserved ...
pnas.org
