So happy this is out as a preprint (for) now. Since measuring ATP is tricky, we used ATP biosensors in collab with @schwarzlanderlab.bsky.social to show that in chloroplasts without PGR5, NPQ and pmf are diminished, yet ATP levels unchanged. Balancing ATP/NADPH must be achieved by different means.
Our new preprint 🚨🌱☀️on flourescent biosensing of ATP levels in chloroplasts in vivo shows disequilibrium between proton motive force and ATP levels. We show PGR5 dependent cyclic electron transfer does not augment ATP/NADPH ratios @gustaf23.bsky.social 🙌 www.biorxiv.org/cgi/content/...