Sample Preparation for Imaging-Based Spatial Transcriptomics in Rigid Plant Tissues (Roots, Shoots) Work by Hanhong Liu and Mingyuan Zhu at Texas A&M University and Jingyuan Zhang at Duke University. #LifeSciences #Reproducibility #PlantScience

A Protocol for Colorectal Tumor Spheroid Culture in Tunable Stiffness Alginate-Based Hydrogels and Subsequent Immunohistochemical Analysis:https://bio-protocol.org/en/bpdetail?id=5786&type=0&source=Bluesky

Automated FLIM-FRET Segmentation Within RNP Condensates Work by Noah D. Powell, Joshua M. Marcus, Leyla E. Fahim and Jason E. Lee at Baylor College of Medicine. #LifeSciences #Reproducibility #CellBiology

direct Stochastic Optical Reconstruction Microscopy to Determine the Oligomeric State of Proteins on the Plasma Membrane and Their Accessibility for Immunotherapeutic Antibodies:https://bio-protocol.org/en/bpdetail?id=5767&type=0&source=Bluesky

Dual Color tau-STED Super Resolution Microscopy in Arabidopsis Root Tip Work by Louise Fougère, Christel Poujol, Yohann Boutté and Magali Grison at Universite de Bordeaux. #LifeSciences #Reproducibility #PlantScience

Liposome-based Expression of the PIEZO1 Sensor GenEPi in Hippocampal Neurons in Organotypic Slices Work by Anya Bhavnani and Olga Kopach at University of London. #LifeSciences #Reproducibility #Neuroscience

DepStep: An Efficient One-Step rRNA Depletion Workflow for RNA Sequencing in Non-model Organisms Work by M. Suleman Qasim and L. Peter Sarin at University of Helsinki. #LifeSciences #Reproducibility #Microbiology

Visualizing the Osteocyte Lacuno-Canalicular System via a Rapid 10-Minute Silver Nitrate Staining Method Work by Jinlian Wu, Weiwei Dai and Libo Wang at Shanghai University of Traditional Chinese Medicine. #LifeSciences #Reproducibility #CellBiology

Coupled Enzyme Assay for Measuring Ornithine Decarboxylase Activity in Cell Lysates Using a Liquid-Stable CO(2) Detection Reagent Work by Jung-Mao Hsu at China Medical University, Taiwan. #LifeSciences #Reproducibility #Biochemistry

A Novel Plate Reader–Based Protocol for Measurement of DNAJB6 Dimerization Activity Work by Anna Gelman, Leif Kofoed Nielsen and Christian Hansen at University College Copenhagen. #LifeSciences #Reproducibility #Biochemistry

Bio-protocol's new issue is now live! 🎉https://bio-protocol.org/en/archive?vol=16&issid=1402 Check out the latest step-by-step protocols in various #LifeSciences fields. #Biochemistry #Biophysics #CancerBiology #CellBiology #Immunology #Microbiology #Neuroscience #PlantScience #StemCell

Separating Chromera velia Zoospores From Culture and Estimating Their Average Motility Speed and Lifespan Work by Jitka Richtová, Dorsaf Ennaceur and Miroslav Oborník at Institute of Parasitology, Biology Centre CAS. #LifeSciences #Reproducibility #Microbiology

An Accurate and Precise ddPCR-Based Method for Determining the Concentration of Plasmid DNA Work by Franco Puleo, Annicka Evans and Cullen Mason at Biogen. #LifeSciences #Reproducibility #MolecularBiology

Isolation of Mononucleated and Binucleated Hepatocytes by Flow Cytometry Work by Yusuke V. Watanabe, Masaki Nishikawa, Yasuyuki Sakai and Takeshi Katsuda at The University of Tokyo. #LifeSciences #Reproducibility #CellBiology

PrimeFlow(TM) Assay for Cell Type–Specific Co-detection of Transgene RNA and Protein in Mouse Spleens From Preclinical Studies:https://bio-protocol.org/en/bpdetail?id=5748&type=0&source=Bluesky

Ex Vivo Assessment of Extracellular Acidification Rate in Murine Intestinal Tissue Work by Alexander F. Lesser and Mitchell L. Drumm at Case Western Reserve University. #LifeSciences #Reproducibility #Biochemistry

Protocol for Measuring Drug–Target Engagement in Mouse Colorectal Cancer Organoids Using NanoBRET Assay Work by Hammed A Badmos, Colin Steele and Ross Cagan at University of Glasgow. #LifeSciences #Reproducibility #StemCell

Gene Editing in Chlamydomonas Using the SCREAM Technique:bio-protocol.org/en/bpdetail... Work by Ian L. Ross and Ben Hankamer at The University of Queensland. #LifeSciences #Reproducibility #PlantScience

Measuring PINK1 Activity in Single Cells Using a PINK1 Kinase Activity Reporter Work by Katie G. Vineall and Danielle L Schmitt at University of California Los Angeles. #LifeSciences #Reproducibility #Neuroscience

A Simplified Langendorff-Based Method for Mouse Cardiac Myocyte Isolation Work by Mie S. Larsen, Morten B. Thomsen and Tamzin Zawadzki at University of Copenhagen. #LifeSciences #Reproducibility #CellBiology

Preparation and Characterization of Neutrophil Membrane-Fused Mitochondria (nMITO):https://bio-protocol.org/en/bpdetail?id=5744&type=0&source=Bluesky Work by Qing Zhang, Yuqin Yue and Xing Zhou at Kunming Medical University. #LifeSciences #Reproducibility #BiologicalEngineering

Iodine Staining of Glycogen Storage in Caenorhabditis elegans:https://bio-protocol.org/en/bpdetail?id=5745&type=0&source=Bluesky Work by Hiba Daghar, Éric Samarut and Alex Parker at Université de Montréal. #LifeSciences #Reproducibility #Biochemistry

Bio-protocol's new issue is now live! 🎉https://bio-protocol.org/cn/archive?vol=16&issid=1401

A Dual-gRNA CRISPR/Cas9 System for Efficient Generation of Large Fragment Deletions in Poplar Work by Guoqian Yang, Yang Yu, Vijaya Kumar Reddy Vulavala, Nidhi Dwivedi and Chang-Jun Liu at Brookhaven National Laboratory. #LifeSciences #Reproducibility #PlantScience

Efficiency-Corrected Relative Quantification of qPCR Data Using LinRegPCR and a Spreadsheet-Based Workflow Work by Louis Arnould Müller and Laurent Tiret at Ecole nationale vétérinaire d’Alfort. #LifeSciences #Reproducibility #BioinformaticsandComputationalBiology

Protocol for In Vivo Two-Photon FCS to Measure Nanocarrier Number and Flow Velocity in Mouse Cerebral Microvasculature Work by Sagar Pande, Xiaojin Wang, Xu Fu, Harini Prathapasinghe and Christopher I. Richards at University of Kentucky. #LifeSciences #Reproducibility #Neuroscience

Stepwise Differentiation of Mouse Embryonic Stem Cells Into Murine Blood Vessel Organoids With Endothelial Lineage Tracing for Quality Control Work by Sophie Guelfi, Sarah Bopp and Gabriele Bergers at VIB-KU Leuven Center for Cancer Biology. #LifeSciences #Reproducibility #StemCell

An Immunoprecipitation-Based Nonradioactive Kinase Assay to Measure Akt Kinase Activity in Mammalian Cell Lines Work by Amber Peek, Jay N. Mehta and Deepali Bhandari at California State University, Long Beach. #LifeSciences #Reproducibility #Biochemistry

Construction and Functional Evaluation of Cyclic Peptide-Based CAR T Cells in Tumor Model Work by Xiaoting Meng, Qingmin Wu and Yu-Hsuan Tsai at Shenzhen Bay Laboratory. #LifeSciences #Reproducibility #CancerBiology

📢 Bio-protocol Webinars present: Engineering next-generation kidney organoids: Enhancing physiological relevance, reproducibility, and scalability 🔗 Register here: bio-protocol.org/register/web...