Check out Ubi-SCAPE! A tonne of hard work and thorough optimisations from Harvey went into getting the most out of the TUBE-based poly-ubiquitin #proteomics workflow; don't worry, we've kept the pre-print short… #TeamMassSpec

Harvey Johnston@harveyejohnston.bsky.social · 8mo ago

Poly-ubiquitylated proteins proved trickier to purify for LC-MS than expected. Non-specific background swamped isolations: with histones and uncertainty! This led us to develop Ubi-SCAPE—for characterising the poly-ubiquitylome to unprecedented purity and depth. www.biorxiv.org/content/10.6...

We're recruiting a Research Assistant (new grant--details to follow!)--come help us work with primary human patient material to make cool 2D/3D co-culture systems, & learn about how #proteostasis & #senescence play together in these contexts: babrahaminstitute.livevacancies.co.uk#/job/details...

A Good Week (TM) for the lab: first big grant, and now first #PhDone.

After all the (re-)connections on day 1, day 2 of #ubfriends2025 even more fabulous for us 🤩 BIG THANKS to all the speakers & poster presenters for sharing their exciting research, and to EVERYONE who joined us! It was a true pleasure to host this amazing community👇

Exciting news: Registration for the 2025 UK Proteotasis & Autophagy meeting (3-5th June) is open: tinyurl.com/mr3p49f9

Joint 2025 Autophagy UK and Proteostasis UK conference | University of Dundee, UK

Welcome to the 2025 joint “Autophagy UK and Proteostasis UK” conference!

tinyurl.com

Online Now: Predictomes, a classifier-curated database of AlphaFold-modeled protein-protein interactions Online now:

Predictomes, a classifier-curated database of AlphaFold-modeled protein-protein interactions

Schmid and Walter train a classifier that discerns functionally relevant structure predictions in proteome-wide protein-protein interaction (PPI) screens using AlphaFold-Multimer, and they use this confidence metric to curate a database of 40,000…

dlvr.it

#Proteostasis strategies diverge between cell states--and this matters! Beautiful, @slavovlab.bsky.social. Concept I first stumbled across in a Ghaemmaghami study (doi.org/10.1073/pnas.1710238114), and it's shaped my science ever since. Treasure-trove of data here for the coming weeks/months/years!

Proteome-wide modulation of degradation dynamics in response to growth arrest | PNAS

In dividing cells, cytoplasmic dilution is the dominant route of clearance for long-lived proteins whose inherent degradation is slower than the ce...

doi.org

Simon Cook@drsimoncook.bsky.social · 2y ago

"our new results and highlights a context-dependent and larger than previously appreciated contribution of protein degradation in shaping protein variation both across the proteome and across cell states" This sounds very interesting