Brighten up your day ✨ New tools from Cagla Eroglu’s lab @caglaeroglu.bsky.social for imaging astrocytes and microglia, centered on Hevin/SPARCL1: https://twp.ai/4huJKq

Power up your protein purification with a new SUMO protease construct from @cdbahl.com and team! Find individual plasmids here: www.addgene.org/browse/artic... Or get the full benchmark set: www.addgene.org/kits/bahl-su...

Chris Bahl@cdbahl.com · 6d ago

The data, code, and detailed protocols are all available on github github.com/aiproteins/h... and all the plasmids are available on addgene www.addgene.org/233685/ www.addgene.org/233686/ www.addgene.org/kits/bahl-su...

✨New glutamine biosensor from @schmittwitt.bsky.social @schmittlab-ucla.bsky.social - thanks for sharing! Find the plasmids here: www.addgene.org/browse/artic...

Schmitt Lab @ UCLA@schmittlab-ucla.bsky.social · 5d ago

The final version of our paper describing the development of iGlo, a glutamine biosensor, is now available. All plasmids are on Addgene! pubs.rsc.org/cb/article/d...

We’re heading to the 2026 BRAIN Initiative Conference in Rockville, MD next week! Come find Marcella Patrick and Meghan Rego. We’ll be talking plasmids, viral vectors, recombinant antibodies, and sharing tools for neuroscience research. 🧠 #BRAINConference #studyBRAIN #Neuroscience

Our CRISPR collection has grown to over 20,000 plasmids from 1,400 labs worldwide 🌎. Together, they offer a fascinating snapshot of the state of the CRISPR field. See more in our latest blog post:

blog.addgene.org

CRISPR Deposits, Distributions, and Other Stats: A Snapshot of the State of CRISPR

twp.ai

Heading to the Genome Engineering: CRISPR Frontiers meeting at @cshlnews.bsky.social next week? Stop by our table to meet @elenamimo.bsky.social and Andrew Hempstead, and check out poster #101 on Wednesday about how researchers share and adopt CRISPR tools through Addgene. #cshlcrispr26 #CRISPR

New from Francois St-Pierre’s lab @bcmhouston.bsky.social! JEDI3sub and JEDI3hyp are voltage indicators that capture subthreshold dynamics under two-photon imaging, so you can image deeper and see more: https://twp.ai/4huJKn

Twenty years ago, Addgene co-founder Melina Fan, PhD, couldn’t get the plasmids she needed for her research, so she helped build a better way to share them. 🧬 On Aug. 1, Melina stepped into a new role as Chief Scientific Advisor. A thank-you and a look at what’s next:

blog.addgene.org

Thank You, Melina: A New Chapter After 20 Years

twp.ai

Great pick for #CellLineOfTheWeek — H2B fluorescently-tagged iPS cells and plasmids from @alleninstitute.org. Proud to help get these into more labs! 🧬

allen institute@alleninstitute.org · 2w ago

Our #CellLineOfTheWeek is Histone H2B! 🧬 Our human iPS cell lines & plasmids – fluorescently-tagged to illuminate structures – are available for just the cost of shipping to accelerate scientific discovery. 📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social

Great to have @allostericsite.bsky.social out at #USCCN this year, meeting the folks keeping small-scale collections going strong.🧫

Eric J. Perkins, PhD (he/him)@allostericsite.bsky.social · 2w ago

Enjoying seeing some familiar faces while representing @addgene.bsky.social at the 2026 United States Culture Collection Network! #USCCN

New from @ipiproteins.bsky.social: a recombinant anti-ROBO2 antibody (IPI-ROBO2.47) targeting a key receptor in Slit-mediated axon guidance. Now available at Addgene, validated for FC and ICC in mouse and human: https://twp.ai/4ht1Pw

Missed our CRISPR pooled libraries webinar? It's on demand. 🎬 Addgene + Broad Institute break down knockout library design, how Julianna & Jacquere were optimized, and how to access them as ready-to-use lentivirus. Watch here:

www.labroots.com

CRISPR Pooled Libraries: Optimizing Knockout Library Design

twp.ai

Not all adhesion molecules are created equal 🤔. NLGN1 is a key synaptic organizer, and you need an antibody that won't cross-react with other neuroligins and muddy your results. @ipiproteins.bsky.social's new recombinant anti-NLGN1 antibody (IPI-NLGN1.15) is now available: https://twp.ai/4ht1Pn

Modeling isn't just for humans — organisms have been modeling for scientists for decades! But not all models are true "model organisms." Learn more about a subset of organisms, called non-models, which can offer unexpected answers to unique questions:

blog.addgene.org

Out of the Box: The Secrets of Non-Model Organisms

twp.ai

Tomorrow! Planning a CRISPR knockout screen? Addgene + Broad Institute's Genetic Perturbation Platform discuss pooled library design — and the newly optimized Julianna & Jacquere libraries for mouse & human. 📅 Jul 22 | 12 PM ET Still time to register: https://twp.ai/4htd2V

Mice! 🐁 Fruit flies! 🪰 Nematodes! 🪱 Naked mole-rats...? What do they have in common? They are models! Or, in the case of the naked mole-rat, a non-model organism. Learn more about non-models in our latest blog post:

blog.addgene.org

Out of the Box: The Secrets of Non-Model Organisms

twp.ai

Did you miss our webinar? It's now on demand.🎬 Addgene and plasmidsaurus break down whole-plasmid long-read sequencing — what it catches that Sanger and short-read NGS can miss, and how to pick the right approach for your workflow. Watch it here:

www.labroots.com

How Well Do You Actually Know Your Plasmid? Long-Read Sequencing and What It Changes for Researchers

twp.ai

On this day in 2012, Addgene received our first CRISPR deposit, from the Doudna lab (Doudna Lab)! pMJ806 became the first plasmid in our CRISPR collection, now a community of 1,400+ labs and 20,000+ plasmids 🧬 Find the plasmid on Addgene:

What separates a good CRISPR screen from a noisy one? Often, the guide design. Next week: Addgene + Broad Institute on the Julianna & Jacquere knockout libraries, built to balance on- and off-target effects. 📅 Jul 22 | 12 PM ET Save your spot: https://twp.ai/4htd26

Tomorrow! Don't miss our free webinar with plasmidsaurus How Well Do You Actually Know Your Plasmid? 📅 July 15 | 12 PM ET Long-read sequencing is changing construct verification — find out what you might be missing. Still time to register: https://twp.ai/4htGfw

Now available: two new plasmid kits from Mark Youles The Sainsbury Laboratory with MoClo parts for tags, promoters, terminators, and more! 🌱 Build expression constructs for your favorite plant system:

🧬🩵Plasmids for all! Thank you @kamounlab.bsky.social and colleagues. Check out all the depositing labs from @thesainsburylab.bsky.social with plasmids at Addgene: www.addgene.org/browse/organ...

Sophien Kamoun@kamounlab.bsky.social · last mo.

Just published: Community Spirit: Plasmids for All How a decade of plasmid-wrangling at @thesainsburylab.bsky.social grew into a movement — and the unexpected friends now helping us open the doors wider. medium.com/p/community-...

Last day of #FENS2026 tomorrow! Excited for two great posters from Addgene collaborators and Ambassadors — details below

Every CRISPR screen starts with one question: which genes actually matter? A knockout pooled library helps answer it, but library design shapes what you can trust. Join us and Broad Institute for a webinar on optimizing library design. 📅 Jul 22 | 12 PM ET Register 👇

www.labroots.com

CRISPR Pooled Libraries: Optimizing Knockout Library Design

twp.ai

Academia, industry, or something in between? Our latest post breaks down how to navigate the PhD job market — translating your skills, avoiding the "apply everywhere" trap, and positioning yourself for the right role. 🧬 Read the full post:

blog.addgene.org

Navigating the PhD Job Market: Choosing Between Academia, Industry, and Everything in Between

twp.ai

Tomorrow at #FENS2026, don't miss Addgene Ambassador Adam Cohen presenting "Imaging Voltage Across Scales" in S34: All-Optical Voltage Imaging for Neuronal Circuit Dissection, Hall B, starting at 9:45am — along with several other talks on voltage sensors.

Plasmid errors are more common than expected, and not every method catches them all. Find the right approach in our webinar with plasmidsaurus 📅 July 15 | 12 PM ET ✅ Sanger, short + long-read ✅ Interpret quality metrics ✅ Pick the right approach Register: https://twp.ai/4htXC1

🧪 Recombinant anti-NRP1 antibodies from @ipiproteins.bsky.social are now available at Addgene. Researchers can use IPI-mNRP1.17 and IPI-mNRP1.23 for FC, IHC, and ICC in mouse and human — with no cross-reactivity to NRP2. 🎯 Check out the antibodies here:

Don't miss Addgene's own poster tomorrow at #FENS2026, July 8! PS04-08PM-680, 2:30-5pm: "Addgene Data Hub: A Platform for Sharing AAV and Antibody Characterization Data" Plus talks and posters from our Ambassadors discussing work done with amazing tools available at Addgene!

Not sure where your PhD fits outside the lab? Whether you're considering academia, industry, nonprofits, or science communication, a focused job search beats applying everywhere. Explore strategies for navigating the PhD job market on our blog:

blog.addgene.org

Navigating the PhD Job Market: Choosing Between Academia, Industry, and Everything in Between

twp.ai