Happy to share our neuronal lysosomal storage disorder toolkit has been published in PNAS! 📚Paper: www.pnas.org/doi/10.1073/...

Felix Kraus@sauerkrausi.bsky.social · 7mo ago

Updated preprint 🧵 We mapped how loss of 23 LSD genes reshapes neuronal proteomes in cortical (iN) and dopaminergic (iDA) neurons New in v2: We added a computational PPI pipeline assessing KO-dependent vulnerability at the interaction and complex level.

Updated preprint 🧵 We mapped how loss of 23 LSD genes reshapes neuronal proteomes in cortical (iN) and dopaminergic (iDA) neurons New in v2: We added a computational PPI pipeline assessing KO-dependent vulnerability at the interaction and complex level.

How do nanoparticles traverse the endothelial barrier in a living organism? We explored this fundamental question using the zebrafish and PEG-based nanoparticles (NPs) in our latest article: (pubs.acs.org/doi/10.1021/...).

Trans-Endothelial Trafficking in Zebrafish: Nanobio Interactions of Polyethylene Glycol-Based Nanoparticles in Live Vasculature

Trans-endothelial transport of nanoparticles remains poorly characterized in live organisms. The zebrafish is a well-established model for direct in vivo imaging; however, standardized controls have n...

pubs.acs.org

Excited to share our latest research, out today in Cell (@cellpress.bsky.social) Using cryo-EM and cell-based studies, we reveal how large nutrient sensing protein complexes form at the lysosomal membrane to turn off mTORC1 and halt anabolic signalling. Read the paper here: doi.org/10.1016/j.ce...

CRN Team Hurley developed AI-directed voxel extraction (AIVE), a segmentation strategy that refines AI-based organelle boundary predictions. Using AIVE, they identified a previously unknown mitochondrial contact termed the mitochondrial intrusion 🧪 🔗 Read the #publication: bit.ly/4qeZiOY

⚡️Mitochondria make ATP, the energy that powers life. But in neurons, with axons up to a meter long, how do these tiny power plants stay functional in the right places? We went looking. 1/n www.biorxiv.org/content/10.6...

Self-renewal of neuronal mitochondria through asymmetric division

Mitochondrial ATP production is essential for life. Mitochondrial function depends on the spatio-temporal coordination of nuclear and mitochondrial genome expression, yet how this coordination occurs ...

biorxiv.org

Thrilled to share that the final piece of my PhD work is now on bioRxiv! biorxiv.org/content/10.1... With support from @nvidia and the @NSF, we used AlphaFold to screen 1.6M+ protein pairs, revealing thousands of potential novel PPIs. All data can be viewed at predictomes.org/hp

Proteome-wide in silico screening for human protein-protein interactions

Protein-protein interactions (PPIs) drive virtually all biological processes, yet most PPIs have not been identified and even more remain structurally unresolved. We developed a two-step computational...

biorxiv.org

Mechanism of #autophagy initiation by transmembrane selective autophagy receptors @elias-adriaenssens.bsky.social & @martenslab.bsky.social review emerging mechanistic differences between soluble and transmembrane autophagy receptors www.embopress.org/doi/full/10....

New from us! Had a question about mitophagy but were afraid to ask? 😂 Excited to review the latest advances in beta cell mitophagy research as a key adaptive response in all forms of diabetes in @cp-trendsendomet.bsky.social. We'd be honored if you have it a read! #T1D #T2D

Trends in Endocrinology and Metabolism@cp-trendsendomet.bsky.social · last yr.

Online Now: Mitophagy in the adaptation to pancreatic β cell stress in diabetes #trends #endocrinology #metabolism

Abstract deadline approaching! Apply for a short/spotlight talk bsky.app/profile/sato...

@satooze.bsky.social · last yr.

Explore emerging research with field leaders @keystoneSymposia.bsky.social #Autophagy : Mechanisms to Therapy, this February in Keystone! keysym.us/KSAutophagy26 #KSAutophagy26 www.keystonesymposia.org/conferences/...

For the love of frontier research, or why Elon’s rockets keep blowing up. The current Zeitgeist favouring utilitarian research risks undermining basic research—the very foundation on which innovative technological advances depend. By Nektarios Tavernarakis: www.embopress.org/doi/full/10....

Check out @antdlewis.bsky.social fun description of organelle contacts here bpod.org.uk/archive/2025... Great work, really enjoyed it. I will now always be imagining that organelles are at at a party! 😄

Biomedical Picture of the Day BPoD@bpodaily.bsky.social · last yr.

Previously unknown contacts between mitochondria identified using new approach with AI & volumetric electron microscopy 📹 Benjamin S. Padman, Runa S.J. Lindblom & @lazaroulab.bsky.social, @monashuniversity.bsky.social @jcb.org ➡️ bpod.org.uk/archive/2025/10/3 with @antdlewis.bsky.social

Position available! Team @mito911.bsky.social needs a new scientific program manager to take over from legendary super-PM @doroteaartscience.bsky.social, who is moving on to bigger things. This is a Berkeley-based position. Apply at aprecruit.berkeley.edu/JPF05103.

Finally in @nature.com 🎉 We uncovered step-wise mTORC1 activation on membrane: RAG–Ragulator recruits, RHEB pushes closer, and direct membrane contacts by RAPTOR + mTOR trigger full kinase activity.👉 The membrane is not just a platform — it’s part of the mechanism. www.nature.com/articles/s41...

Structural basis for mTORC1 activation on the lysosomal membrane - Nature

Cryo-electron microscopy was used to study human mechanistic target of rapamycin complex 1 (mTORC1) activation on lysosomal membranes, showing progressive recruitment by RAG–Ragulator, RHEB and R...

nature.com

📅 Less than 2 weeks left to submit your abstract for our 6th Annual Symposium Webinar! 🎉Don’t miss this chance to share your work, hear the latest advances in autophagy research, and connect with a vibrant community! 🎉 The link for abstraction submission is below 👇 forms.office.com/r/wZbTvPePwR

😺 to see this one out @jcellsci.bsky.social & have a 🌲 planted for it): journals.biologists.com/jcs/article/... am sure more creative people will make better use of this resource 1/2

Excited to share our new paper in Nature Communications: Evidence that Mitochondria in macrophages are destroyed by microautophagy 👉 rdcu.be/eDa1S

💫NEW: @molinarilab-er.bsky.social & co examine organellophagy receptor features, finding modules of functionally conserved intrinsically disordered regions and defining shared features despite sequence divergence, such as a net negative charge, that promote fragmentation. bit.ly/4fLWe99

The intrinsically disordered regions of organellophagy receptors are interchangeable and control organelle fragmentation, ER-phagy and mitophagy flux - Nature Cell Biology

Rudinskiy et al. examine organellophagy receptor features, finding modules of functionally conserved intrinsically disordered regions and defining shared features despite sequence divergence, such as ...

bit.ly

Dr Linh Dang and I penned this commentary on our own article that explores cell-type specific #autophagy in humans. We discuss #aging, sex, nutrient restriction, and the technology we need to better understand this process in people: www.tandfonline.com/doi/full/10.... @sahmri.bsky.social

Cell type-specific autophagy in human leukocytes: signatures of aging, sex, and nutrient restriction

Macroautophagy (referred to here as autophagy) is thought to play a critical role in aging and age-related disease, making it a priority for development of targeted human therapies. We developed a ...

tandfonline.com

Selective autophagy of organelles: the “disorder” is key. Intrinsically disordered regions of organellophagy receptors are interchangeable, required, and sufficient for organelle fragmentation and lysosomal delivery … and all about ORGATACs 😉 rdcu.be/ey8iL

The intrinsically disordered regions of organellophagy receptors are interchangeable and control organelle fragmentation, ER-phagy and mitophagy flux

Nature Cell Biology - Rudinskiy et al. examine organellophagy receptor features, finding modules of functionally conserved intrinsically disordered regions and defining shared features despite...

rdcu.be

Want to share your work at the premiere meeting of the minds between basic membrane biology and therapeutics? Short talk deadline is coming up Oct. 8, 2025. www.keystonesymposia.org/conferences/...

And it's out! I'm thrilled to share our new paper (Adriaenssens et al., Nat Cell Biol 2025). This paper describes a new mechanism for the initiation of autophagosome biogenesis. We found that this WIPI-ATG13-driven pathway is preferentially used by a group of transmembrane autophagy receptors.

Structural and cell biology NPG publication two-fer today: lysosome signaling structural biology tinyurl.com/mt3uc96k with @robzonculab.bsky.social and mitophagy initiation tinyurl.com/3rpjyfjt with @martenslab.bsky.social @lazaroulab.bsky.social and @hummerlab.bsky.social

Delighted to share our latest work looking at the role of the RNA-binding proteins Clu1 (yeast)/Clu (flies) in regulating the translation of #mitochondria proteins. Really interesting regulator of mito biology. Huge, long-running effort by PDRA Leonor Miller-Fleming 👏 dx.plos.org/10.1371/jour...

Clu1/Clu form mitochondria-associated granules upon metabolic transitions and regulate mitochondrial protein translation via ribosome interactions

Author summary Mitochondria are essential cellular organelles that perform many important roles in regulating metabolism. They are dynamic in form, function and composition which is crucial to maintai...

dx.plos.org

In search for some autophagy summer reading? Check out the full Journal of Molecular Biology special issue «Autophagy accross scales - from molecules to physiology» edited by Sharon Tooze and myself: www.sciencedirect.com/journal/jour... @arcticautophagy.bsky.social @womeninautophagy.bsky.social

ATZ polymers elicit intervention of CNX:FAM134B-LC3II segregation complexes + formation of VAPA-ORP1L-RAB7 membrane contact sites to facilitate Syntaxin18/VAMP8-driven fusion of ER and endolysosomal membrane. One of the ER-to-Lysosome-Associated Degradation flavors. www.biorxiv.org/content/10.1...

The involvement of the ER-phagy receptor FAM134B in membrane contact sites between ER and endolysosomes promotes ERLAD

Membrane contact sites (MCS) between organelles maintain the proximity required for controlled exchange of small molecules and ions yet preventing fusion events that would compromise organelles identi...

biorxiv.org

Impact of federal funding cuts on science, the progress of knowledge, and our future - an essay by our own Steve Liberles: magazine.hms.harvard.edu/articles/i-t...

“I Think the Entire Future of the United States Is at Stake”

magazine.hms.harvard.edu

Very excited to share my postdoc work- out now at PNAS! Learn more about how neurons facing chronic autophagic stress compensate by upregulating two secretion pathways Autophagic stress activates distinct compensatory secretory pathways in neurons | PNAS www.pnas.org/doi/10.1073/...

WSTF nuclear autophagy regulates chronic but not acute inflammation is published in Nature😀 nature.com/articles/s41... Congratulations to all collaborating groups! Fantastic effort by first author Yu Wang! Kudos to Zixhun Dou for leading the entire project!😀