lncRNAs regulate gene expression by forming RNA•DNA:DNA triplexes. NMR spectroscopic exchange rate analysis reveals thermodynamic parameters of the Watson-Crick base pair opening, uncovering how antiparallel triplex formation by HIF1α-AS1 RNA modulates Adrenomedullin DNA stability bit.ly/4fhoC4O

We propose the sequence of the nascent transcript influences the processivity of RNAP II transcription via Hrp1. Hrp1 bound to the RNAP II elongation complex increases processivity. Hrp1 bound to RNA bit.ly/49zoJoC

A genome-independent LC-MS/MS approach selectively detects modified oligonucleotides, overcoming traditional sequence-dependent limits. #tRNAModifications #RNAModificationMapping #LCMSMS bit.ly/4o1oqJ1

F. tularensis ribosomes contain 1 of 3 bS21 paralogs. We found that stability and translation of the bS21-2 mRNA, rpsU2, are controlled post-transcriptionally and depend on the mRNA leader sequence bit.ly/49yDqrT

4SeUTP (yellow color) was first synthesized, and recognized by T7 RNA polymerase. The transcribed 4SeU-hammerhead ribozyme facilitates the catalysis likely via the enhanced product releasing bit.ly/3Sc4t6k

Mutations (asterisks) in the multicopy genes for U6 and U4 spliceosomal RNAs are associated with the vision disorder retinitis pigmentosa. Prior studies in yeast suggest a possible mechanism bit.ly/435xfbi

The 3′ UTR isn’t just a tail. Shorter regions boost translation termination, longer ones can hinder it — but structured elements rescue the process by bringing key factors together at the ribosome bit.ly/3QfcEyh

(Left) Reduced ADAR1 heightens type I IFN. (Right) ADAR1 deficiency confers resistance to Plasmodium yoelii despite CD8⁺ T cell loss, with CD4⁺, B, Treg, γδ T and NK cells compensating bit.ly/4nWbFzs

A global view of RNA/DNA interactions in complex 3D structures from PDB, including Leontis/Westhof basepair annotation, pseudoknots, base-backbone interactions, and handling 800+ modified nucleotides bit.ly/4dG6EaX

The pseudouridine synthase Pus4 induces tRNA conformational changes through its catalysis and binding. This study reveals the chronology of these changes using biochemical and biophysical methods bit.ly/4x0O8kX

Human telomerase RNA (hTR) can exist in the full-length form or in a 5’-truncated form. Loss of the 5’ domain can occur through either endogenous RNA processing or antisense oligo-induced degradation bit.ly/4wZvwSo

A method of comprehensive sequencing analysis of the small RNA fragmentome (RiboMarker) bit.ly/4uGk7oX

mRNA COVID-19 Vaccines: Science vs. Misinformation bit.ly/48jPtbN

Pathogen-derived pathoPEPs could mimic plant miPEPs, thereby controlling the expression of plant miRNAs and their target genes, and promoting infection bit.ly/3O2CGUf

Search for known loop motif 3D structures in Rfam with JAR3D bit.ly/41ctfEQ

We interpret global and targeted mouse Exosc10 deletion phenotypes in the context of the gene’s clinical potential as a prognostic cancer biomarker and explore its transcriptional regulatory network bit.ly/41IYUOj

What’s next for RNA biology at system scale? This editorial frames a shift toward quantitative, integrative approaches, highlighting advances in measuring, modeling, and manipulating RNA across biological systems #RNAbiology #SystemsBiology #Genomics #RNAseq #ComputationalBiology bit.ly/41hv9UJ

RNA doesn’t just encode information, it helps build cellular structure. This review explores how RNAs drive the formation of biomolecular condensates, shaping organization, regulation, and function across contexts #RNAbiology #PhaseSeparation #BiomolecularCondensates #CellBiology bit.ly/4mjjI8B

RNA isn’t just regulated in time—it’s regulated in space. This review explores mechanisms of mRNA localization and localized translation, highlighting how spatial control shapes cellular function and specialization #RNAbiology #mRNAlocalization #GeneRegulation #CellBiology #RNAseq bit.ly/4eboUJF

How do we connect RNA structure to function? This review highlights new approaches to probe RNA structure across dimensions, linking structural features to RNA activity, interactions, and regulation in complex cellular contexts #RNAbiology #RNAstructure #Genomics #ComputationalBiology bit.ly/4sthgOt

Can we drug RNA? This review explores the landscape of RNA-targeting small molecules, highlighting strategies to identify binding sites, design ligands, and overcome challenges posed by RNA structural flexibility #RNAbiology #RNAstructure #ChemicalBiology #DrugDiscovery #Genomics bit.ly/4sv5Dq1

RNA function is defined by its interactions. This review surveys cutting-edge methods to map protein–RNA and RNA–RNA contacts, outlining how we can now begin to reconstruct RNA interaction networks at transcriptome scale #RNAbiology #Interactome #RNAprotein #Genomics #SystemsBiology bit.ly/41KDfWl

How well can machine learning predict RNA secondary structure? This review surveys current approaches highlighting both impressive gains and key limitations in generalizability and interpretability #RNAbiology #MachineLearning #RNAstructure #ComputationalBiology #Genomics bit.ly/4spSj6s

Nanopore direct RNA sequencing is redefining how we detect RNA modifications and damage—enabling long-read, transcriptome-wide views with minimal RNA handling. This perspective highlights DRS in probing the epitranscriptome #RNAbiology #Epitranscriptomics #Nanopore #RNAseq #Genomics bit.ly/4dx1lec

What does it mean to study RNA at system scale? This perspective lays out a roadmap—from quantitative transcriptomics to integrative modeling—for how we measure, model, and ultimately manipulate RNA across biological systems #RNAbiology #SystemsBiology #Genomics #ComputationalBiology bit.ly/3POaGo9

Excited to highlight the new Special Issue of RNA showcasing advances in system-level RNA biology, from epitranscriptomics and RNA damage to deep learning–driven structure prediction and beyond #RNAbiology #Epitranscriptomics #RNAseq #ComputationalBiology #Genomics bit.ly/4tEQL9I

Two amino acids in each homologous PUF repeat make molecular contacts that drive base recognition. These molecular contacts cannot generally be functionally interchanged between repeats, as shown bit.ly/3OrhTtq

The proteome is dynamically remodeled across the mammalian cell cycle. Here, we review the mechanistic basis and functional consequences of cell cycle-specific mRNA decay and translational control bit.ly/4atoJYd

Translation elongation stresses like tRNA synthetase inhibitors induce the stress response but inhibit translation elongation, leading to a parabolic dose-response pattern of stress granule assembly bit.ly/4aANDUi

Complementary DNA oligonucleotide direct in-gel quantification (cDINGQ) for precise tRNA fragment analysis bit.ly/3ZEmn2g