🟧 Orange is the new green?? 🟧 New sensitive orange calcium indicator (🟠OCaMP) now out in Nature Comms: www.nature.com/articles/s41... Highlights ⚡ Detects single action potentials in vivo 🔬 Optimized for 1030 nm imaging 📸 Highly photostable, minimal photoswitching 🌈 Multiplex with green biosensors

Glutamate is the primary molecule that neurons use to communicate with each other. Previously, scientists have mostly recorded when neurons fire output signals, but now with a new glutamate indicator, they can record the many inputs that causes cells to fire. 🧵 #neuroskyence

iGluSnFR4 is now available @natmethods.nature.com www.nature.com/articles/s41... Glutamate indicators with increased sensitivity and tailored deactivation rates 🧪

Glutamate indicators with increased sensitivity and tailored deactivation rates - Nature Methods

iGluSnFR4f and iGluSnFR4s are the latest generation of genetically encoded glutamate sensors. They are advantageous for detecting rapid dynamics and large population activity, respectively, as demonst...

nature.com

🚨iGluSnFR4 is finally out!🚨🧪 We present iGluSnFR4f and 4s, a novel pair of genetically-encoded glutamate indicators designed for high-fidelity imaging of synaptic activity in the living brain. ⬇️ www.biorxiv.org/content/10.1... 🎥 Below: iGluSnFR4s detecting minis in cultures w/ TTX #Neuroscience

High-speed video showing glutamate lighting up as it’s released in synapses, visualizing the neurotransmitters of the brain with the fluorescent indicator protein iGluSnFR3. Credit: Allen Institute, Aggarwal et al.

First sky post 🦋 Sharing some vibrant iGluSnFR3 minis in cultured neurons!