Analytical and Quantitative Light Microscopy Course
@aqlm.bsky.social
This is the account for the Analytical and Quantitative Light Microscopy Course at the Marine Biological Laboratory in Woods Hole MA. We love all things microscopy!
#AQLM Series🧵: Microscopy Mistakes -- oh, FRAP! What happens after a #fluorescent molecule is bleached with a laser? In FRAP #microscopy, the recovery itself is the measurement. 🔬 📸 @3i Intelligent Imaging Innovations on the Marianas platform; 63× objective and a Photometrics Prime 95B camera. (1/7)
Happy #MicroscopyMonday - #AQLM students doing single particle tracking using QD655 with THE Louise on a Leica DMi8 Inverted Widefield Microscope! Thank you for all your years at #AQLM, Louise! #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪
Back by popular demand: Pets of AQLM, round two! Already looking ahead to the 2027 Pet Cohort. 🐾 #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥 #STEMCommunity 🫂
From Eel Pond, a community of #microscopic life comes into view. Captured in darkfield at 4X, 0.16 NA on an #Evident DP75 microscope, each branching form is defined by the light it scatters, revealing delicate edges and structure against a dark field. #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬
#ThrowbackThursday with #AQLM. Since its founding in 1980 by Shinya Inoué, AQLM has been closely tied to the evolution of #imaging living systems. Today, it continues to train new generations in quantitative biological imaging. #MBL 🌊 #Microscopy 🔬 #BioImaging 🧬 #WoodsHole 🦞 #Optics 💡#Confocal
Happy #MothersDay from this shrimp and her eggs, captured on an #Evident DP75 4X/0.16 NA darkfield microscope. #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy
That’s a wrap on #AQLM 2026 🔬Thank you to everyone who made this year’s course possible: students, faculty, TAs and vendors. We are looking forward to what this #microscopy community builds next. Interested in applying next year? 2027 applications open in late 2026, stay tuned for updates.
It was an honor to hear Prof. Sarah Veatch, PhD, deliver yesterday’s Inoué Lecture, discussing how spatiotemporal pair-correlation analysis can recover nanoscale membrane organization in live-cell single-molecule localization #microscopy despite diffusion and molecular motion. #MBL 🌊 #AQLM 🧫
Why be out of focus when you could be at #AQLM, learning about microscopes inSTED? Today, our students did rotations with our vendors #OxfordInstruments, #Nikon, #3i, #Leica & #ONI, learning about super-resolution #imaging modalities including DNA-PAINT and dSTORM. #MBL 🌊 #Microscopy 🔬
Good to have you back again this year, Jim! @thejimsims.bsky.social #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🔥 #STEMCommunity 🫂 #ScientistLife 🧑🔬
@thejimsims.bsky.social · 3mo ago
We tuned into a lecture by #AQLM course director, Prof. Diane Lidke, PhD, about single particle tracking. She discussed labelling strategies including quantum dots, and their applications using 2D/3D trajectory visualizations and diffusion mapping. #MBL 🌊 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼
Yesterday, we tuned into a lecture by Prof. Reto Fiolka, PhD, about Light-Sheet #Fluorescence #Microscopy. He discussed light sheet #optics and different #imaging modalities, as well as implementations in live imaging whole #embryos. #MBL 🌊 #AQLM 🧫 #BioImaging 🧬#WoodsHole 🦞 #LightMicroscopy 🧪
@carsten-wolff.bsky.social teaching @aqlm.bsky.social students about light sheet #microscopy on the @zeiss-microscopy.bsky.social LightSheet 7. Feat. cute crustacean embryo for specimen
This sleek beam was created through a fluorescence cube on a Cairn OpenFrame, using a Toptica laser. ✨ #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥 #STEMCommunity 🫂
Nickelback said to "look at this graph": A rail-mounted light sheet #microscope creating a sharp intensity peak. So cool! #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥
We are @mblscience.bsky.social @aqlm.bsky.social sharing the latest live cell imaging technology with our Marianas SDC system. 3i's LaserStack, Vector3 TIRF/FRAP, and components from ...
Real-time imaging at #AQLM, where “woooo got a signal” is a totally valid reaction! 🔬 #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥 #STEMCommunity 🫂 #ScientistLife 🧑🔬
These bright “comets” mark growth, where EB3 tracking shows the advancing ends of microtubules in real time. ☄️ 📸 EB3 dynamics captured on the 3i Marianas #imaging system with a 63× objective and Prime BSI 95B camera. #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #Fluorescence🪼 #Optics 💡
How your microscope spreads light: a guide to point spread functions. 💥 (1/5) 📸 #AQLM Day 4 Lab GIF! #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥 #STEMCommunity 🫂
We attended “Digital Detectors” by Dr. Simon Watkins, PhD. Advances in sCMOS #technology reduce noise, improving sensitivity and dynamic range. With better coatings, Nyquist-matched pixels, and optimized illumination, light loss is minimized and image quality is improved. 🌊 #MBL #AQLM #Microscopy
#Cells showing #mitochondria in red, actin in green, and nuclei in blue: a hustling, bustling city of powerhouses, scaffolds, and headquarters 🏭🛣🏢. Captured at 40× on an #Evident BX53 epifluorescence #microscope with a #Hamamatsu ORCA-Flash 4.0 LT3. #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Fluorescence🪼
All planes in focus, all fields in view. 4×4 EDF mosaic from 16 z-stacks, captured with DIC at 20× on a #Nikon Eclipse NiE with a #Hamamatsu ORCA-Fusion camera. #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪
Hydrozoa from Eel Pond @mblwoodshole imaged in dark field on an @evidentmicroscopy upright #microscope (cool to be able to tile and stitched on the go with a manual stage). These cuties are related to jellyfish, sea anemones and corals. 📸 Photo and caption credit: Nat Prunet @microscopic_farmer
“We’re gonna need a bigger scope” Rabbit muscle fibers imaged with DIC @aqlm.bsky.social #microscopy #jaws @mblscience.bsky.social
Polarization time! Rabbit muscle fibers imaged on a polarization microscope @aqlm.bsky.social #microscopy #labelfree
