An Accurate and Precise ddPCR-Based Method for Determining the Concentration of Plasmid DNA Work by Franco Puleo, Annicka Evans and Cullen Mason at Biogen. #LifeSciences #Reproducibility #MolecularBiology
Bio-protocol
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Bio-protocol (www.bio-protocol.org) was founded in 2011 by a group of researchers at Stanford University with a singular mission: Improving research reproducibility by publishing high-quality protocols in the life sciences.
Isolation of Mononucleated and Binucleated Hepatocytes by Flow Cytometry Work by Yusuke V. Watanabe, Masaki Nishikawa, Yasuyuki Sakai and Takeshi Katsuda at The University of Tokyo. #LifeSciences #Reproducibility #CellBiology
PrimeFlow(TM) Assay for Cell Type–Specific Co-detection of Transgene RNA and Protein in Mouse Spleens From Preclinical Studies:https://bio-protocol.org/en/bpdetail?id=5748&type=0&source=Bluesky
Ex Vivo Assessment of Extracellular Acidification Rate in Murine Intestinal Tissue Work by Alexander F. Lesser and Mitchell L. Drumm at Case Western Reserve University. #LifeSciences #Reproducibility #Biochemistry
Protocol for Measuring Drug–Target Engagement in Mouse Colorectal Cancer Organoids Using NanoBRET Assay Work by Hammed A Badmos, Colin Steele and Ross Cagan at University of Glasgow. #LifeSciences #Reproducibility #StemCell
Gene Editing in Chlamydomonas Using the SCREAM Technique:bio-protocol.org/en/bpdetail... Work by Ian L. Ross and Ben Hankamer at The University of Queensland. #LifeSciences #Reproducibility #PlantScience
Measuring PINK1 Activity in Single Cells Using a PINK1 Kinase Activity Reporter Work by Katie G. Vineall and Danielle L Schmitt at University of California Los Angeles. #LifeSciences #Reproducibility #Neuroscience
A Simplified Langendorff-Based Method for Mouse Cardiac Myocyte Isolation Work by Mie S. Larsen, Morten B. Thomsen and Tamzin Zawadzki at University of Copenhagen. #LifeSciences #Reproducibility #CellBiology
Preparation and Characterization of Neutrophil Membrane-Fused Mitochondria (nMITO):https://bio-protocol.org/en/bpdetail?id=5744&type=0&source=Bluesky Work by Qing Zhang, Yuqin Yue and Xing Zhou at Kunming Medical University. #LifeSciences #Reproducibility #BiologicalEngineering
Iodine Staining of Glycogen Storage in Caenorhabditis elegans:https://bio-protocol.org/en/bpdetail?id=5745&type=0&source=Bluesky Work by Hiba Daghar, Éric Samarut and Alex Parker at Université de Montréal. #LifeSciences #Reproducibility #Biochemistry
Bio-protocol's new issue is now live! 🎉https://bio-protocol.org/cn/archive?vol=16&issid=1401
A Dual-gRNA CRISPR/Cas9 System for Efficient Generation of Large Fragment Deletions in Poplar Work by Guoqian Yang, Yang Yu, Vijaya Kumar Reddy Vulavala, Nidhi Dwivedi and Chang-Jun Liu at Brookhaven National Laboratory. #LifeSciences #Reproducibility #PlantScience
Efficiency-Corrected Relative Quantification of qPCR Data Using LinRegPCR and a Spreadsheet-Based Workflow Work by Louis Arnould Müller and Laurent Tiret at Ecole nationale vétérinaire d’Alfort. #LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Protocol for In Vivo Two-Photon FCS to Measure Nanocarrier Number and Flow Velocity in Mouse Cerebral Microvasculature Work by Sagar Pande, Xiaojin Wang, Xu Fu, Harini Prathapasinghe and Christopher I. Richards at University of Kentucky. #LifeSciences #Reproducibility #Neuroscience
Stepwise Differentiation of Mouse Embryonic Stem Cells Into Murine Blood Vessel Organoids With Endothelial Lineage Tracing for Quality Control Work by Sophie Guelfi, Sarah Bopp and Gabriele Bergers at VIB-KU Leuven Center for Cancer Biology. #LifeSciences #Reproducibility #StemCell
An Immunoprecipitation-Based Nonradioactive Kinase Assay to Measure Akt Kinase Activity in Mammalian Cell Lines Work by Amber Peek, Jay N. Mehta and Deepali Bhandari at California State University, Long Beach. #LifeSciences #Reproducibility #Biochemistry
Construction and Functional Evaluation of Cyclic Peptide-Based CAR T Cells in Tumor Model Work by Xiaoting Meng, Qingmin Wu and Yu-Hsuan Tsai at Shenzhen Bay Laboratory. #LifeSciences #Reproducibility #CancerBiology
📢 Bio-protocol Webinars present: Engineering next-generation kidney organoids: Enhancing physiological relevance, reproducibility, and scalability 🔗 Register here: bio-protocol.org/register/web...
Whole-Mount Immunostaining of Tyrosine Hydroxylase for Dopaminergic Neuron Analysis in Zebrafish Larvae Work by Luís Félix at University of Trás-os-Montes and Alto Douro. #LifeSciences #Reproducibility #Neuroscience
Generation and Characterization of Adaptive Anoikis-Resistant Cells Using Cyclic Attachment-Detachment Culture of Cancer Cells Work by Resha Rajkarnikar, Mehri Monavarian and Karthikeyan Mythreye at The University of Alabama. #LifeSciences #Reproducibility #CancerBiology
Multiply Perturbed Response: A Computational Protocol to Identify Cooperative Allosteric Residue Combinations Driving Protein Conformational Transitions: bio-protocol.org/en/bpdetail?...
An Optimized Protocol for the Characterization of Zebrafish ApoB-Containing Lipoproteins Using the LipoGlo System Work by Monica R. Hensley and Steven A. Farber at Johns Hopkins University. #LifeSciences #Reproducibility #Biochemistry
Electrophoretic Mobility Shift Assay (EMSA) for Assessing RNA–Protein Binding and Complex Formation Using Recombinant RNA-Binding Proteins and In Vitro–Transcribed RNA: bio-protocol.org/en/bpdetail?...
DiRT v2.0: An Optimized Pipeline for Detecting Dicistronic tRNA-mRNA Transcripts in Plants: bio-protocol.org/en/bpdetail?...
Amplification-Free Detection of Highly Structured RNA Molecules Using SCas12aV2:https://bio-protocol.org/en/bpdetail?id=5462&type=0&source=Bluesky
Visualizing Diverse RNA Functions in Living Cells With Spinach(TM) Family of Fluorogenic Aptamers Work by Ryan O’Hanlon and Karen Y. Wu at Lucerna. #LifeSciences #Reproducibility #MolecularBiology
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Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture Work by Eleanor I. Lamont, Richard M. Jones, Jessica Assadi, Shuyi Ma and David R. Sherman at University of Washington. #LifeSciences #Reproducibility #Microbiology
Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data Work by Maya N. Walker, Bo Hu, Shi-Yuan Cheng and Xiao Song at Northwestern University Feinberg School of Medicine. #LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
