Acoustofluidic cryo-EM enables in situ particle manipulation for uniform, high-quality cryo-EM specimens pubmed.ncbi.nlm.nih.gov/42777058/ #cryoEM

I'm excited to share our new preprint, where we show that fine-tuning gold nanoparticle conjugation to different Fab structural elements can produce powerful, rigidly coupled, labeling tools for in-situ cryo-ET. Take a look if you want to see an AuNP in an EM map!🔬🧠 www.biorxiv.org/content/10.6...

Lab bonding at a Timbers game! (we made it on TV sitting behind the goal, if you squint 😂)

I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell. authors.elsevier.com/a/1mA6h,LqAr...

Out today, we can now multiplex receptor mapping at synapses thanks to a dimeric gold nanoparticle label optimized by Hoyoung that's distinguishable from monomers! Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...

Excited to share the final version of this work from the group, led by Chengli! Always fun when an unexpected complex appears in a cryo-EM dataset 😀 rdcu.be/eym9v

Gating and noelin clustering of native Ca2+-permeable AMPA receptors

Nature - Cryo-electron microscopy analyses of calcium-permeable AMPA receptors from rat brain show that they are composed primarily of GluA1 and GluA4 subunits, and biochemical studies show that...

rdcu.be

I've been lucky to be able to work with Natalie on producing several new monoclonal antibodies using these methods, with great success! Check out our group's new protocols paper on how to generate mAbs for various membrane proteins - currentprotocols.onlinelibrary.wiley.com/doi/full/10....

Generation of Conformation‐Specific Monoclonal Antibodies for Integral Membrane Proteins

Antibodies and their antigen-binding fragments, including fragment antigen-binding domains (Fabs) and single-chain variable fragments (scFvs), are extraordinary tools in all fields of biology, partic....

currentprotocols.onlinelibrary.wiley.com

So glad to see this out! Congrats!

Aleksandra Skrajna@askrajna.bsky.social · 2y ago

My most challenging postdoctoral project, where we show that histones but not nucleosomes are polyubiqutinated by the APC/C for a potential proteasomal degradation was just published in @naturecomms.bsky.social 🎉 www.nature.com/articles/s41... Big shout-out to all co-authors!!!

Having a great week so far at #BPS2025!

Congrats Makayla!

Makayla Freitas@makayla-freitas.bsky.social · 2y ago

Thrilled to share the pre-print of my dissertation research on the bile acid-sensitive ion channel (BASIC)! 🥳

Gotta love finding soccer balls in tomograms ⚽ 😂

Loving the holiday spirit at PNCC to start off December 🎄 + early Xmas gift getting to test out Tomo5!

First Bluesky post! Our recent eLife paper is now an official version of record. This was my first project using #Cryo-ET and I'm definitely hooked - it's so fun to look at snapshots of synapses within brain tissue and see individual proteins. Excited to share more soon! doi.org/10.7554/eLif...

Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses

By harnessing antibody fragments conjugated to small gold nanoparticles together with high pressure freezing, FIB/SEM milling and cryo-electron tomography, AMPA receptors were identified from mouse br...

doi.org