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Reconstitution of lamin assembly on nuclear pore complex-containing membranes

Intermediate filaments called lamins line the metazoan nuclear envelope and organize the nucleus and genome. Unlike actin and microtubules, purified intermediate filament proteins assemble into non-ph...

journals.biologists.com

nature.com

Characterization of Vlf1 as a regulator of lipophagy

Lipid droplets (LDs) are unique organelles, surrounded by a phospholipid monolayer. They are present in most eukaryotic cells including the unicellular model organism S. cerevisiae . LDs store neutral...

biorxiv.org

CPAP/CENPJ is essential for the stability and function of ESCRT-pathway associated AAA+ ATPase VPS4B

Function of CENPJ/CPAP is essential for centriole duplication and cilia biogenesis, disruption of which can lead to microcephaly and Seckel syndrome. Recently, we showed that CPAP is an integral Endos...

biorxiv.org

Reconstitution of multistep recruitment of ULK1 to membranes in autophagy

The Human ULK1 autophagy-initiating complex consists of ULK1, FIP200, and the HORMA domain heterodimer ATG13:ATG101. PI3P is essential to recruit ULK1C to membranes for ULK1, but ULK1C subunits do not...

biorxiv.org

Structural basis of fatty acid activation and transport by human FATP2 - Nature Communications

Here, the high resolution cryo-EM structures of human FATP2 in both the pre-catalytic and intermediate states, combined with biochemical validations, reveal a catalysis-dependent mechanism for FATP2-m...

nature.com

Principles of ribosome-associated protein quality control during the synthesis of CFTR - The EMBO Journal

Prolonged translational arrests caused by defective mRNAs activate the ribosome-associated protein quality control (RQC) pathway, which marks harmful incomplete proteins for degradation. Multipass tra...

link.springer.com

A phosphorylation switch governs KIF11’s mechanical output during mitosis | PNAS

The kinesin-5 motor protein KIF11 is crucial for mitotic spindle assembly, driving the separation of spindle poles through microtubule sliding. Src...

pnas.org

Cornichon receptors couple membrane adaptation to cargo selection during ER export

Selective export of membrane proteins from the endoplasmic reticulum (ER) is fundamental for eukaryotic cell biology, yet how trafficking receptors coordinate cargo recognition with membrane adaptatio...

biorxiv.org

Sorcin couples Annexin A11 recruitment to ESCRT-III assembly for plasma membrane repair | PNAS

The absence of a cell wall affords animal cells diverse functionality at the cost of acute sensitization to plasma membrane (PM) damage. Thus, anim...

pnas.org

In-situ cryo-ET of mouse embryos reveals cytoplasmic lattices contain ubiquitin-charged E2-E3 ligase assemblies - The EMBO Journal

Cytoplasmic lattices (CPLs) are filamentous assemblies essential for mammalian embryonic development. They are known to regulate organelle organization, spindle assembly, and protein homeostasis, but ...

link.springer.com

Structure of NHE6 and its lipid-mediated interactions regulating endosomal pH - Nature Communications

The sodium-proton exchanger NHE6 localises to endosomal membranes and mutations in the protein are known to cause the X-linked neurological disorder Christianson syndrome. Here the authors report four...

nature.com

The structural basis for LRRK2’s activation and autoinhibition

Elevated kinase activity in leucine-rich repeat kinase 2 (LRRK2) is linked to Parkinson’s disease; this study shows that its ROC GTPase acts as a nucleotide-dependent molecular switch that regulates a...

cell.com

A tool to pulse-label yeast nuclear pore complexes in imaging and biochemical experiments

A non-perturbative nanobody-based strategy was developed for pulse-labeling yeast nuclear pore complexes to selectively capture Nup84-containing complexes for imaging and biochemical analysis.

elifesciences.org

nature.com

STAT5A–α-actinin axis links actin integrity to mitochondrial homeostasis and innate immune activation

Here, Sun et al. demonstrate that loss of the transcription factor STAT5A reduces α-actinin-1 expression, leading to collapse of the actin cytoskeleton. Th

rupress.org

Allosteric switching of Ca2+ permeability in the lysosomal ion channel TPC2 underpins biased agonist activation - The EMBO Journal

Ion channels possess selectivity filters that are hardwired to ensure the selective passage of ions. Lysosomal two-pore channels are unusual as they are able to switch their cation selectivity in an a...

link.springer.com

A molecular switch for coordinating kinesin and dynein transport of mitochondrial cargo

The cellular distribution of mitochondria in response to stress and local energy needs is governed by the relative activities of the microtubule-based molecular motors kinesin and dynein. The mechanis...

science.org

Volume EM reveals desmosome three-dimensional architecture in epithelial cells and tissue models

Bharathan et al. provide the first comprehensive description of the 3D desmosome in A431 epidermoid carcinoma, human mammary epithelial cells, and airway e

rupress.org

Bilayer thickness and transmembrane domain dynamics drive directional ER-Golgi traffic via Erv14/cornichon

Directionality of protein traffic during secretion is driven by sorting receptors that bind cargo in one compartment and release them in another. Erv14/cornichon family receptors mediate delivery from...

biorxiv.org

Vimentin remodeling in response to oxidants and electrophiles is modulated by pH

Vimentin filament remodeling by cysteine reactive agents is tuned by pH changes, illustrating pH dependence of redox signaling.

science.org

Cell size reduction distinctly scales spindle elongation and chromosome segregation in C. elegans - Nature Communications

Embryos must adapt to decreasing cell size. Although chromosome segregation. dynamics in C. elegans remain largely invariant, spindle length scales with cell. size and can be recapitulated by only var...

nature.com

α-Synuclein blocks endoplasmic reticulum co-translational protein translocation early in Parkinson’s disease - Nature Communications

Proteomic and transcriptomic profiling on hiPSC-derived midbrain dopaminergic neurons with pathological α-synuclein burden reveals defective Sec61A interactions and co-translational translocation of E...

nature.com

DCAF11-dependent molecular glue degrader activated by glutathionylation - Nature

M12 is a metabolically activated molecular glue that recruits diverse proteins to DCAF11 for ubiquitin-mediated degradation, revealing a glutathione-dependent mechanism that broadens targeted protein ...

nature.com

An in vivo examination of Dynein-Cargo complex formation.

Long-range intracellular transport relies on microtubule motors. This process is particularly important in large cells such as neurons and oocytes. While transport towards the plus-end of microtubules...

biorxiv.org

ER cholesteryl ester phase separation underlies switch-like cholesterol sensing

Cellular lipid homeostasis requires mechanisms that detect subtle changes in lipid abundance and trigger rapid, coordinated responses. The INSIG/SCAP/SREBP2 pathway provides a central feedback system ...

biorxiv.org

Activity-dependent astrocytic cholesterol release fine-tunes modes of synaptic exo- and endocytosis

The role of astrocytic cholesterol signaling in modulating presynaptic function remains poorly understood. Using dynamic nanoscale-precision imaging in hip

rupress.org

A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation

Autophagy degrades cellular material by sequestering it within autophagosomes, which form de novo from precursors called phagophores. Phagophore assembly and expansion require ATG9A-positive seed comp...

biorxiv.org

The ARLA1A-NAC089 module coordinates ER phagy with the unfolded protein response to maintain cellular homeostasis in plants

Yang et al. reveal that the ARLA1A-NAC089 module couples ER phagy with the UPR in plants. This dual regulatory axis fine-tunes cell survival versus death under stress, providing a mechanistic framewor...

cell.com