Segata Lab

@cibiocm.bsky.social

The Laboratory of Computational Metagenomics led by Nicola Segata **We are hiring, get in touch if interested!** http://segatalab.cibio.unitn.it/ Moderator @svetlanaup.bsky.social

🎉 Exciting news from our lab! cFMDv1.3.2 is now live. This release marks a significant expansion of cFMD: 📊 681 newly integrated food metagenomes from 22 public datasets 18,180 total MAGs across 4,125 metagenomes 54 countries (now including 🇲🇦) 100+ food-related studies 1/3

🎉 Exciting news from our lab! cFMDv1.3.2 is now live. This release marks a significant expansion of cFMD: 📊 681 newly integrated food metagenomes from 22 public datasets 18,180 total MAGs across 4,125 metagenomes 54 countries (now including 🇲🇦) 100+ food-related studies 1/3

We have an unusual situation this year: we have more mentors than usual for our current number of Singletons! Please share the link with your network! We will match up as many as we can! #ASMicrobe #MicroSky 🦠🧪🔬

Binning Singletons@binningsingletons.com · 2mo ago

Microbe @asm.org attendees! The matching registration form is locked but you can still join the community! forms.gle/5roddkPnjuBt... Join us on Slack and IRL. We often gain up to an additional 50% more people after we've made matches! #ASMicrobe #MicroSky 🔬🧪🦠🏳️‍🌈

How well do ARG detection pipelines agree when applied to the same data? Spoiler: not very well. In our new preprint, we ran 10 pipelines on 270M microbial unigenes from GMGCv1. The same data can support conflicting biological conclusions! 🧵 www.biorxiv.org/content/10.6...

The elusive resistome: a global comparison reveals large discrepancies among detection pipelines

Identifying antibiotic resistance genes (ARGs) from metagenomic data is critical for studying antimicrobial resistance across microbial communities and pathogens. However, there is no standardized methodology for ARG annotation. Here, we compare ten commonly used ARG detection pipelines by analysing over 270 million prokaryotic genes from the Global Microbial Gene Catalogue across 13 distinct habitats. We observed up to a 45-fold difference in the number of reported ARGs, with a mean Jaccard index of only 16% between pipelines. Pipeline selection profoundly impacted downstream biological interpretations, with drastic changes to estimates of ARG relative abundance and richness, to the characterization of pan- and core-resistomes, and to the class-level composition of the inferred resistome. ARG detection pipelines make different, defensible trade-offs, and no single approach should be treated as authoritative. Therefore, users should justify and communicate choices carefully, as our analyses show that, taken uncritically, the same data can support conflicting biological and ecological interpretations. ### Competing Interest Statement The authors have declared no competing interest. National Health and Medical Research Council of Australia (NHMRC), 2031902 Australian Research Council (ARC), FT230100724 International Development Research Centre (IDRC), 109304-001 Deutsche Forschungsgemeinschaft (DFG), FO1279/6-1 Bundesministerium für Bildung und Forschung (BMBF), F01KI1909A, 01KI2404B Swedish Research Council (VR), 2024-06123, 2019-00299, 2023-01721 Knut and Alice Wallenberg Foundation, KAW 2020.0239 Swedish Foundation for Strategic Research, FFL21-0174

biorxiv.org

NEXT WEEK Chioma Onyido and I are happy to be part of #IBC2026 where we will share our #BugSigDB @bioconductor.bsky.social experience!

International Biocuration Conference 2026@intbioconf2026.bsky.social · 7mo ago

🧬 Workshop @ #IBC2026 | Cape Town Community-Driven Biocuration for Microbiome Research 📅 Apr 20, 2026 | 👩‍🔬 Dr S. Ugarcina Perovic & Ms Chioma Oselu ⚠️ Conference registration required 📝 Register: www.bioinformaticsinstitute.africa/form/worksho...

Have you listened to the #MVIFconversations podcast? There is a new episode where @cpavloud.bsky.social and I talked to @merenbey.bsky.social about open source, mentoring, and believe me it's a GREAAAT one!!

Microbiome Virtual International Forum@microbiomevif.bsky.social · 4mo ago

#MVIFconversations podcast episode 3 with @merenbey.bsky.social is OUT! ▶️ Spotify: open.spotify.com/episode/1Cke... ▶️ Amazon Music: music.amazon.com/podcasts/81c... ▶️ Apple Music: podcasts.apple.com/us/podcast/m... ▶️ YouTube podcast: youtu.be/-qC3qSU884U?... 1/2