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Developmental Biology

@devbiol.bsky.social

Official Journal of the Society for Developmental Biology www.sciencedirect.com/journal/developmental-biology

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Developmental Biology @devbiol.bsky.social · yesterday

#DBfeature #ICYMI Genetic knockout suggests Isl1 enhancer redundancy in mouse hindlimb development By Seth Olson, Yasuhiko Kawakami, et al. tinyurl.com/2jjje2sn

Isl1 HLPE contributes to ISL1 expression in the branchial arch ectoderm
(A) Representative images of the wild-type (A-A‴) and Isl1 HLPE−/− (B-B‴) E9.5 branchial arch stained with DAPI (A, B), anti-TRP63 antibody (A′, B′), and anti-ISL1 antibody (A″, B″). Panels A‴ and B‴ show merged images. The white dotted line in A‴ and B‴ represents the region of interest where fluorescent intensity was analyzed. Scale bar: 100 μm. Single-color images are shown in grayscale.

Developmental Biology @devbiol.bsky.social · 2d ago

#DBfeature #LatestResearch 🪰 The Drosophila ovarian terminal filament imports lipophilic molecules that support cyst and follicle development within the ovariole By Bhawana Maurya, Allan Spradling doi.org/10.1016/j.ydbio.2026.06.013

Kinetics of Sec6 and LpR2 dependent lipid transfer from terminal filament to the germarium. LpR2_KD knockdown (bab1-GAL4,tub-GAL80ts > LpR2-RNAi) in terminal filament and cap cells on adult day1 and analyzed on day3, leads to a reduction in lipid droplet number within the germarium, a smaller region 1 and 2a with less-branched fusomes (dashed outline), and defects in cyst development.

Developmental Biology @devbiol.bsky.social · 5d ago

#DBfeature #ICYMI The epigenetic factor CFP1 regulates BMP signaling during limb development, expanding and maintaining mesenchymal progenitors and initiating chondrogenesis. By Emanuele Pignatti, Lijie Jiang, Diana Carlone, et al. tinyurl.com/y3xevhew

Whole mount in situ hybridization analysis of Sox9 gene expression in control (Ctrl) and cKOPrx1 forelimb buds. White dashed line outlines the forelimb bud.

Developmental Biology @devbiol.bsky.social · 6d ago

#DBfeature #LatestResearch The vascular–osteogenic interface in craniofacial development: a structured review of emerging associations in congenital malformations By Karl Jacobs et al. doi.org/10.1016/j.ydbio.2026.06.012

Formation of the craniofacial skeletal structures in the developing head. Regions are distinguished based on neural crest or mesodermal origin, as well as the embryological mechanism of bone formation (Reprinted from Journal of Cranio-Maxillofacial Surgery, Vol 45, Kruijt Spanjer et al., ©2017, with permission from Elsevier Kruijt Spanjer et al., 2017).

Developmental Biology @devbiol.bsky.social · last wk.

#DBfeature #ICYMI H3K9me2-modified chromatin regions rearrange during mouse embryonic development to adopt cell type-specific configurations By Diana Fulmer, Emily Shields, Andrey Poleshko, Jonathan Epstein, Cheryl Smith tinyurl.com/3e4xutw8

A model of lineage-specific chromatin reorganization concordant with lineage-specific gene expression during cell fate adoption. (A) The model illustrates the correlation between chromatin reorganization and activation of lineage-specific genes (in LADs) and lineage-specific enhancers (in KODs) that occurs during cell differentiation. (B) A schematic illustration of lineage-specific transcriptional enhancer organization in a KOD. Enhancers linked to a given promoter are indicated. In precursor cells (top), enhancers in a KOD are in an inactive, H3K9me2-modified state. During cell differentiation, enhancers undergo local loss of H3K9me2 and acetylation of H3K27 to allow for gene expression.

Developmental Biology @devbiol.bsky.social · last wk.

#DBfeature #LatestResearch 🪰 Germline cysts require Ecdysone receptor for proper timing of encapsulation in the Drosophila ovary By Lindsay Swain, Daniel Phipps, Susannah Catherine Webster, Lauren Jung, Elizabeth Ables doi.org/10.1016/j.ydbio.2026.06.008

Knockdown of EcR in the germline delays egg chamber formation. (A-C) osk-Gal4>UASp-lacZ control (A), EcRRNAi (B), labeled with Vasa (green) and anti-Hts and anti-LaminC (magenta). Cysts are outlined in white.

Developmental Biology @devbiol.bsky.social · last wk.

#DBfeature #ICYMI Intraocular vasculature formation precedes extraocular vasculature penetration of mouse eyes By Satoshi Imanishi, Yohei Tomita, Kazuno Negishi, Kazuo Tsubota, Toshihide Kurihara tinyurl.com/y38awzd3

Formation of hyaloid capillaries and artery. Whole mount staining in mouse eyes at different times. (A) A section of whole mount staining for αSMA, EMCN and TUBB3 in E 11.5 eye. (B) The separated EMCN signal from (A). Cells in hyaloid cavity are EMCN+ (arrow). However, no EMCN + structure has penetrated the retina. (C) Whole mount staining for αSMA, EMCN and TUBB3 in E 12.5 eye. (D) The separated EMCN signal from (C). One EMCN + vessel has penetrated the retina (arrow), but vasculature along the optic nerve has not penetrated the eye. (E) Whole mount staining for EMCN (green) and MAP2 (magenta) in E 13.5 eye. An EMCN + vessel runs along optic nerve. (F) The vessel become αSMA + hyaloid artery at E 15.5. 12 embryos from 3 different mothers were used for E 11.5, 12.5 and 13.5 experiments. 11 embryos from 3 different mothers were used for E15.5 experiments. Scale bar: 200 μm.

Developmental Biology @devbiol.bsky.social · 2w ago

#DBfeature The duplication-degeneration-complementation model: A seminal framework for evolutionary retention of duplicated genes By Chris Amemiya doi.org/10.1016/j.ydbio.2026.06.006

Concept diagram of the DDC model (adopted and modified from Prince and Pickett, 2002). In this simple schematic, a single gene (grey box) is shown with three cis-elements (pink, green and blue boxes). After the gene undergoes "Duplication" respective elements that are redundant are able to undergo "Degeneration," wherein mutations (red X's) can render the elements nonfunctional. Since the original gene regulatory functions are maintained in this "Complementation" phase, the gene duplicates are both kept due to this subfunctionalization.

Developmental Biology @devbiol.bsky.social · 2w ago

#DBfeature #ICYMI 🐸 Identification, expression and function of 28 new candidate regulators of placodal neurogenesis in Xenopus laevis By Bertrand Hutlet, Gerhard Schlosser tinyurl.com/34szsky6

Expression patterns of eya1 and its candidate interaction partners at mid-tailbud stage. Xenopus laevis embryos after whole-mount in situ hybridization at stage 27, in lateral views (anterior is to the left). The schematic drawing (A) shows the distribution of placodes in a mid-tailbud stage embryo. Arrows indicate expression in placodes and display the colour code used in the drawing (yellow: profundal placode; dark green: trigeminal placode; light green: olfactory placode, pink: otic placode; brown: lateral line placode; orange: epibranchial placodes). For placodes derived from the posterior placodal area, only the otic placode and one lateral line placode are indicated in panels C–L. Arrowheads and asterisks indicate other areas of expression. Pink arrowhead: somites; white asterisks: pharyngeal pouches. Blue asterisks (neural crest streams) and arrowheads indicate expression in areas where eya1 is not expressed. Panels D, F, H, J, L are magnifications of panels C, E, G, I, K, respectively. Abbreviations: B, blood islands; E, prospective eye, including lens (L) placode; EB, epibranchial placodes; F: forebrain; H: hindbrain; LL, lateral line placodes; LPM, lateral plate mesoderm; M: midbrain; Ol, olfactory placode; Ot, otic placode; P, pronephros; Pr, profundal placode; V, trigeminal placode.

Developmental Biology @devbiol.bsky.social · 2w ago

#DBfeature A tribute to Gail R Martin, mouse geneticist and mentor extraordinaire (April 12, 1944 - Feb 9, 2026) By Alexandra Joyner doi.org/10.1016/j.ydbio.2026.05.007

Gail Martin looking at the camera, sitting on a bench in front of a lily pad pond in her garden in Berkeley. Photo from Brigid Hogan.

Developmental Biology @devbiol.bsky.social · 2w ago

#DBfeature #ICYMI 🐸 MicroRNA miR-196a controls neural crest patterning by repressing immature neural ectoderm programs in Xenopus embryos By Alice Godden, Grant Wheeler, et al. tinyurl.com/4mne33bu

Depletion of miR-196a expands neural plate progenitors at the expense of neural crest specification and neuronal differentiation. Embryos were injected into one dorsal blastomere at the 4-cell stage of development with 300 pg of GFP mRNA or of lacZ developed with Red-gal. In all panels the injected side is to the right. Whole mount in situ hybridisation of zic1, (stage 14), zic3, (stage 14), msx2, (stage 14), sox2, (stage 14), pax6, (stage 15) hairy1, (stage 18), eng2, (stage 15) n-tub (stage 18) and elrc1, (stage 18), following MO mediated miRNA KD. Embryos are positioned in dorsal view with anterior at the top. Whole mounts are shown as well as transverse sections (i). Injection of the MO caused an expansion in the NPB markers Zic1 (n = 28/30) and Zic3 (n = 34/37) but a loss in the anterior region of the NPB marker Msx2 (n = 18/19) (A–C). The neural marker Sox2 was expanded in a lateral direction and appeared to lose the intense dark region of expression seen in the control side (see black arrow) (n = 37/37) (D). Expression of the neural marker Pax6 was slightly enlarged across the anterior neural fold (lens primordia) (see white arrow) but reduced in the position of the forebrain stripe (see black arrow) (n = 18/24) (E). The downstream target of Notch – Hairy1 had a reduced but broadened expression (n = 22/25) (F) Eng2 showed a reduced and broadened expression as well as a shift more posterior (n = 19/25) (G). Both neuronal differentiation markers N-tub (n = 24–30) and ELRC (n = 23/24) were reduced following miR-196aMO injection although this was restricted to the anterior most part of the NP (see black arrows). The expression of these two markers in the trigeminal placodes had extended in both an anterior and posterior direction (see white arrows) (H,I).

Developmental Biology @devbiol.bsky.social · 2w ago

#DBfeature #LatestResearch 🧠 Microtubule binding protein Togaram1 is required for proper development of mammalian forebrain and neural primary cilia, with its knockout leading to a thin and irregular neuron layer By Clarissa Nassar, Savera Shetty, Noelle Dwyer doi.org/10.1016/j.ydbio.2026.05.011

Neuron layer of E12.5 Togaram1 KO cortex is thin and irregular.
(A) Control E12.5 cortical section shows a thick layer of Pax6+ NSCs in the ventricular zone topped by daughter cells forming layers of basal progenitors (Tbr2+) and neurons (Tubb3+).
(B-D) Examples of different neuron layer abnormalities found in Togaram1 KOs.
(B) KO #1 displays a deep ventricular indent and a small neuron cluster to the left of the indent. Neuron layer is thinner above the indent, but is continuous.
(C) KO #2 has a very thin patchy neuron layer with a large neuron cluster.
(D) KO #3 has a ventricular indent with a thin neuron layer with a gap above the indent. White dashed line marks ventricle, yellow dashed lines outline ectopic neuron clusters, yellow arrowheads indicate breaks in the neuronal layer. Scale bars: 100um.

Developmental Biology @devbiol.bsky.social · 3w ago

#DBfeature #ICYMI Injury-induced Neuregulin-EGFR signaling from muscle mobilizes stem cells for whole-body regeneration in acoels By Brian Stevens, Riley Popp, Heather Valera, Kyle Krueger, Christian Petersen tinyurl.com/4h52at7f

Model. (A) Early wound signaling activates expression of nrg-1 in muscle near the wound site by 6 hours and subsequently in the distal blastema region from 1 to 5 days. As regeneration progresses, nrg-1 expressed from muscle signals through egfr-1, and receiving cells of this signaling could either be neoblasts (gray arrows), muscle, or other cell types. A downstream consequence of the signaling results in localization of neoblast proliferation near the blastema region, which correlates in space and time approximately with the locations of nrg-1's late expression phase. The activity of nrg-1 and egfr-1 promotes blastema outgrowth. (B) Regulatory model identifying a critical role for nrg-1 and egfr-1 functionally downstream of egr. Wounding activates immediate early expression of egr, which is responsible for transcriptional activation of nrg-1 and runt-1. nrg-1 is functionally required for regeneration and acts through egfr-1, and these factors exert critical responses downstream of egr in order to localize proliferation to wound sites and also cause blastema outgrowth. Gray lines: contributions from nrg-1 signal feedback and/or roles for pre-existing NRG-1 protein in the wound activation process (see text). (C) Phylogeny showing the conserved use of Neuregulin and/or EGFR signaling to functionally promote regeneration. Acoels are believed to be either early-branching Deuterostomes within Xenacoelomorpha or form a sister group to all Bilaterian animals (dotted lines).

Developmental Biology @devbiol.bsky.social · 3w ago

#DBfeature #LatestResearch 🐣 Gene knockout in chick reveals a new in vivo role for Cxcl14 during trigeminal ganglion development By Hugo Urrutia, Junpeng Gao, Marianne Bronner doi.org/10.1016/j.ydbio.2026.06.001

Perturbation of Cxcl14 specifically in placode cells disrupts trigeminal ganglion development. A. Schematic illustrating the in ovo experimental design to perturb Cxcl14, specifically targeting placode cells. Experimental (red) reagents were delivered unilaterally into the ectoderm of HH11 (13 somites) chick embryos. B-E″. Either a Cxcl14 specific gRNA-CRISPR/Cas9 construct (B-C′) or Cxcl14 MO (D-E′) were introduced onto the right side of the embryo. Immunolabeling of SOX10 (magenta) and TUJ1 (green) reveals the effects of loss of Cxcl14 within the trigeminal ganglion, specifically in placode cells at HH16. Arrows indicate position of neural crest-derived cells. Arrowheads indicate position of placode-derived cells.

Developmental Biology @devbiol.bsky.social · 3w ago

#DBfeature #ICYMI 👁️ Unraveling the developmental heterogeneity of human retinal ganglion cells within the developing retina By Emil Kriukov, Jonathan Soucy, Everett Labrecque, Petr Baranov tinyurl.com/mtvnssdy

Schematic of workflow. Public datasets re-analysis; processing (UMAPs shown); integration and downstream analysis (cartoon of cell fate trajectories and cell-cell interactions); generation of a reference atlas (annotated UMAP); application by sequencing new data and automatically embedding in the atlas and annotating cell types.

Developmental Biology @devbiol.bsky.social · 3w ago

#DBfeature #LatestResearch 🧠🐁 Neurogenesis and gliogenesis in the developing neocortex are imbalanced in mice lacking the proteoglycan Tsukushi By Sabrina Quaresima, Valerio Licursi, Tenta Ohkubo, Giuseppe Lupo, et al. doi.org/10.1016/j.ydbio.2026.05.009

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Alt text
TSK is expressed in the cortical plate of the developing mouse neocortex.
(A to E) Immunofluorescence with an anti-β-gal antibody (A, B), or X-gal staining (C to E), in coronal brain sections of E14.5 (A), P0 (B, C), P14 (D), or adult (E) TSK+/- mice, showing β-gal expression (green in (A, B); light blue in (C to E)) in the cortical plate between E.14.5 and P14. Hoechst nuclear staining is shown in blue (A, B). Scale bars, 500 μm (A, B) or 1000 μm (C to E).
(F to O) Double immunofluorescence in coronal brain sections of E14.5 TSK+/- mice with an anti-β-gal antibody (green staining) and anti-PAX6 (F, K), anti-Nestin (G, L), anti-TBR2 (H, M), anti-βIII-Tubulin (I, N) or anti-TBR1 (J, O) antibodies (red staining), showing β-gal expression in the cortical plate, partially overlapping with the TBR1+ domain. Images in (K to O) show magnifications of the boxed areas in (F to J). Hoechst nuclear staining is shown in blue. Scale bars, 500 μm.

Developmental Biology @devbiol.bsky.social · 3w ago

#DBfeature #ICYMI 🐠 Single, double, and triple mutant combinations reveal redundant and novel functions of scube genes during zebrafish development By Quoc Duy Tran, Peter D. Currie et al. tinyurl.com/3d8tj7sf

scube mutants display Hh loss-of-function phenotypes. Loss of slow muscle fibres in scube mutant families at 30 hpf. In wild-type zebrafish, slow muscle fibres form a regular array with clearly defined V-shaped somites. In scube1−/−, scube3−/−, and scube1−/−scube3−/−, morphology of slow muscle fibres are comparable to wild-type siblings. In contrast, scube2−/−, scube1−/−scube2−/−, scube2−/−scube3−/−, and the triple scube mutant show severe disruption of slow muscle fibres along the body, including fibre loss (white arrows), abnormal fibre shapes (yellow arrows), and the presence of U-shaped somites (indicated by white dashed lines). The triple mutant shows the most severe phenotype, characterised by the near-complete loss of slow muscle fibres and pronounced abnormalities in the remaining fibres.

Developmental Biology @devbiol.bsky.social · 4w ago

#DBfeature #LatestResearch 🐠 Skeletal remodeling is responsible for the foramen in the skull for the hyoid artery in zebrafish By Miki Iwasaki, Junpei Kuroda, Masanori Nakae, Hironori Wada doi.org/10.1016/j.yd...

Formation of the Ch foramen by bone deposition. Bone, cartilage and endothelial cells are labeled in cyan, green and magenta, respectively. (E′) A single optical section of the boxed region in E. Channel for green and magenta fluorescence is shown in the right panel. n = 3. Anterior is to the left.

Developmental Biology @devbiol.bsky.social · 4w ago

#DBfeature #ICYMI 🪱 C. elegans sperm activation for fertilization requires robust activity of the TAT-5 lipid flippase By Katherine Maniates, Ann Wehman et al. tinyurl.com/5fu7c49e

Cartoon of wild type and tat-5 mutant mature sperm and cell membrane. Wild type sperm is motile and functional thanks to internalised PtdEth in the membrane. Mutant spermatocytes lose membrane asymmetry without tat-5 activity, losing their activation an motility.

Developmental Biology @devbiol.bsky.social · 4w ago

#DBfeature #LatestResearch 🐠 Regulation of the yolk microtubule and actin cytoskeleton by Dachsous cadherins during zebrafish epiboly By Gina Castelvecchi, Lila Solnica-Krezel, et al. tinyurl.com/58dmfkc7

Bundled yolk microtubule array structures in dchs triple mutants A. Confocal images at 50% epiboly of WT and dchs triple mutants stained with DAPI (blue) and for DM1α (microtubules, green). Arrowhead indicates microtubule bundling. B. Quantification of yolk microtubule bundling through measurement of bare yolk areas lacking microtubules at 50% epiboly. ∗∗p < 0.01. WT N = 25 embryos, MZdchs1a−/−; Zdchs1b−/−; MZdchs2−/− N = 32 embryos. C. Confocal images of yolk microtubule meshwork marked with DM1α, just vegetal of YSL of WT and dchs triple mutants at 50% epiboly. D. Quantification of yolk microtubule alignment coherency. ∗∗∗∗p < 0.0001. WT N = 25 embryos, MZdchs1a−/−; Zdchs1b−/−; MZdchs2−/− N = 32 embryos.

Developmental Biology @devbiol.bsky.social · 4w ago

#DBfeature #ICYMI 🪱 Expression of pal-1, the C. elegans Caudal homologue, is required for correct positioning of motor neuron cell bodies in the larval ventral nerve cord and is regulated by the nuclear hormone receptor SEX-1 By N Noblett, T Roenspies, S Flibotte, A Colavita tinyurl.com/2u963pvx

(E) Schematic showing DD and DA neuron organization along the VNC. (F) Representative images of anterior DA and DD neurons in pal-1(zy117), sex-1(zy130) and double mutants. Asterisks mark dorsally mispositioned neurons. Scale bar = 20 μm. (G) Quantification of worms with dorsally mispositioned neurons at L1. Statistics: (C) one-way ANOVA with Tukey's Post-hoc Comparisons, and (G) Chi-squared test with Monte Carlo simulation for 10,000 replicates, followed by a pairwise analysis using Fisher's exact test with Monte Carlo simulation for 10,000 replicates and adjusted with Holm corrections.

Developmental Biology @devbiol.bsky.social · last mo.

#DBfeature #LatestResearch 🐠 A parallel reporter assay for zebrafish enables simultaneous, quantitative analysis of multiple transcriptional reporters, and cell type-specific comparisons of cis-regulatory module activity By Gloria Ligunas, Stefan Materna tinyurl.com/357w6usv

Parallel reporter analysis reveals cell-type–specific CRM activity in sorted vascular endothelial cells. Activity of barcoded reporters was quantified in sorted vascular endothelial cells relative to the inactive control (C120). Reporters containing the fli CRM showed the strongest activation in this cell type. ubb-containing reporters were significantly activated over controls, but to a lesser extent than in whole embryos. dll4in3 reporter activity was elevated relative to baseline, although the difference from C120 was not statistically significant. Each CRM was tested using two independent barcoded reporters across four biological replicate experiments. Individual points represent barcode-specific measurements from independent biological replicates.

Developmental Biology @devbiol.bsky.social · last mo.

#DBfeature #ICYMI Loss of CMTR1 leads to gastrulation failure and disrupted anterior-posterior patterning, with unique gene expression profiles between XX and XY embryos By Janelle Welton, Kimberly Tremblay, Jesse Mager tinyurl.com/4ruh8b5k

Cmtr1 KOs exhibit sex specific differences (a) Whole mount E7.5 male and female control and KO embryos. (B) Quantification of embryo size from E7.5 litters using ImageJ. (C) Principal component analysis (PCA) indicating male and female KOs cluster separately. (D) Normalized counts for select non-X or –Y linked differentially expressed genes which are higher in male KOs than female KOs. (E) Normalized counts (DESeq2) for select differentially expressed non-X or –Y linked genes which are higher in female KOs than male KOs. (F) RT-PCR validation of male/female differential expression in mutant embryos

Developmental Biology @devbiol.bsky.social · last mo.

#DBfeature #LatestResearch Single-cell RNA-seq uncovers cellular diversity and dynamic gene regulatory networks along the progenitor-to-chondrocyte trajectory of late synovial joint development By Ji-Hye Yea, Qin Bian, Patrick Cahan, et al. tinyurl.com/3cwut5ft

Dynamic GRNs associated with inferred transcriptional progression between progenitor-like and chondrogenic states. (A) Heatmap of genes dynamically expressed along the chondrocyte trajectory. Epoch divides cells (columns) and genes (rows) into stages or epochs. Results of enrichment analysis of genes up-regulated in each epoch are shown to the right of the heatmap. The Epoch algorithm also reconstructs dynamic gene regulatory networks (GRNs). The top regulators of each epoch, determined by the network importance metrics of centrality and betweenness, are listed to the right of the enrichment results. (B) Sub-networks of that exemplify genes specific to the early (Clec3b) and late (Col9a3 and Prg4), and their regulators.

Developmental Biology @devbiol.bsky.social · last mo.

#DBfeature #ICYMI Review: Critical roles of the primitive endoderm in in fetal cell fate specification and patterning By Marcelio Shammami, Alyssa Virola-Iarussi, Ian McCrary, Amy Ralston tinyurl.com/dy5js2f7

PrE signaling before and after implantation. A) During preimplantation development, spontaneous differences in FGF4/FGFR1,2/MEK signaling sort PrE from EPI. B) After implantation and prior to gastrulation, the anterior visceral endoderm (AVE) secretes antagonists of BMP/Nodal/WNT signaling to direct anterior/posterior patterning.

Developmental Biology @devbiol.bsky.social · last mo.

#DBfeature #LatestResearch Camk2g1 regulates Notch signaling and histone methylation to specify hematopoietic stem cells By Camden Kurtz, Sanyam Patel, Haerin Kim, Sarah Rothschild tinyurl.com/bdp9u37v

Elevated H3K27me3 levels in camk2g1 crispants. Increased levels of H3K27me3 are observed in the dorsal aorta (DA, white arrows) but not the posterior cardinal vein (PCV, green arrows) of camk2g1 fli1:GFP positive morphants (n = 24-30) compared to control embryos (n = 29-30)

Developmental Biology @devbiol.bsky.social · last mo.

#DBfeature #ICYMI Review: Controllable targeted protein degradation as a promising tool for discovery of novel cellular and developmental mechanisms By De-Li Shi, Xuan Zhao, Chengtian Zhao, Ming Shao tinyurl.com/yc26fzy6

Schematic of the function of common protein degradation systems DeGradFP, AID, dTAG, and PROTAC.

Developmental Biology @devbiol.bsky.social · 3mo ago

#DBfeature Loss of potassium channel Kcnj2 alters electrical activity in palate mesenchyme cells, which affects a network of calcium-induced transcription factors and causes cleft palate in mice. By Yunus Ozekin, Rebecca O'Rourke, Emily Anne Bates tinyurl.com/4c9w8saz

Loss of Kcnj2 affects transcription in Osr1 positive mesenchyme 4 cells. Immunohistochemistry shows that Asporin expression is expanded into more cells in Kcnj2KO/KO palatal shelves. A white line shows where fluorescence was quantified and the arrow shows the region of highest asporin expression.

Developmental Biology @devbiol.bsky.social · 3mo ago

#DBfeature 🐸 TMBIM4 affects left-right patterning via pluripotency exit during gastrulation in X. tropicalis, and its loss replicates the phenotypes of human congenital heart disease patients by Nicholas Diab, Mustafa Khokha et al www.sciencedirect.com/science/arti...

GFP-Tmbim4 localizes to the membrane in Xenopus

Developmental Biology @devbiol.bsky.social · 3mo ago

#DBfeature 🧑‍🎓 The Neural Tube CURE trains students in developmental biology methodology while offering a research course designed to increase opportunities for undergraduate research experience By Anneke Dixie Kakebeen, Joshua Fandel, Lee Niswander tinyurl.com/y2va2xpy

The neural tube course based undergraduate research experience
Scientific goal. Spina bifida: congenital defect, worldwide impact. Candidate human bifida genes --> validate candidate genes in chick embryos.
Course gaols. Developmental biology, research & society, techniques, science communication.
Student outcomes. Self-evaluations show significant gains in concepts, techniques, literature, writing, presenting, self-efficacy.
Experimental workflow. sgRNA/Cas9 in HH4 and HH10 chick embryo, look for neural tube defects.