Excited to share our work with @julietrolle.bsky.social & Jef Boeke. We developed a 'shotgun' method to screen millions of synthetic metabolic pathways to enable mammalian cells to grow without two essential nutrients for the first time in >500 million years! doi.org/10.1101/2025... Thread...
A shotgun approach for highly multiplexed mammalian metabolic engineering
Mammalian metabolic engineering is critical to advancing basic biology, bioproduction, and cell therapy. However, as pathway complexity increases, so does the size of both the combinatorial design spa...
doi.org