Sharper footprints, cleaner insights? Yuxuan Lin at the University of Pennsylvania explores a new approach that corrects Tn5 bias for bulk & single-cell ATAC-seq, boosting the accuracy of transcription-factor binding 🔬 👉 www.biorxiv.org/content/10.1... #SingleCell #Epigenomics #ATACseq

Robust footprinting with sample-specific Tn5 bias correction for bulk and single cell ATAC-seq

Footprint analysis of assay for transposase-accessible chromatin via sequencing (ATAC-seq) enables base resolution mapping of regulatory elements but is often underpowered and prone to false positives...

biorxiv.org

🚀 Excited to share SMART-PTA (miniaturized Primary Template Amplification) a high-throughput single-cell whole-genome amplification method that preserves clonal lineage information while enabling deep genomic and transcriptomic profiling.

Dan Landau@landau.bsky.social · 10mo ago

Shout out to Tamara Prieto, Dennis Yuan, John Zinno and @ivanraimondi.bsky.social . Thank you to our funders at @themarkfdn.bsky.social

🧬 We’re excited to introduce D&D-seq, a single-cell technology that maps DNA:Protein interactions through molecular footprinting. Check it out here: biorxiv.org/content/10.1... #Genomics #Epigenetics

Single-cell mapping of regulatory DNA:Protein interactions

Gene expression is coordinated by a multitude of transcription factors (TFs), whose binding to the genome is directed through multiple interconnected epigenetic signals, including chromatin accessibil...

biorxiv.org