Hamed made a very nice summary of my new preprint about our new method and study for finding target sites of TFs in in vitro context, thus reflecting target sites without contribution of chromatin.

Hamed Najafabadi@hsnajafabadi.bsky.social · 2y ago

(14/n) Make sure to check out the manuscript to see how these findings advance our understanding of TF sequence specificity and evolution. This massive effort was led by @arttujolma.bsky.social, Aldo Hernandez-Corchado, Ally W.H. Yang, Ali Fathi, Kaitlin U. Laverty and others not yet on Bluesky.

(1/8) 🚀 Excited to share our findings on a large-scale ChIP-seq assay for 166 previously uncharacterized human transcription factors (TFs) and their roles in both regulatory regions, and more strikingly, the “dark matter” genome. 🌌 doi.org/10.1101/2024....

Extensive binding of uncharacterized human transcription factors to genomic dark matter

Most of the human genome is thought to be non-functional, and includes large segments often referred to as “dark matter” DNA. The genome also encodes hundreds of putative and poorly characterized tran...

doi.org

Here is a Blueskytorial describing our new work.

@timhughesto.bsky.social · 2y ago

(1/7) Thrilled to reveal the results of the Codebook Project, an international effort to identify accurate DNA-binding motifs and genomic binding loci for the >300 uncharacterized human transcription factors (TFs) doi.org/10.1101/2024...