Out now in Nature Communications! We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky www.nature.com/articles/s41...

It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...

bioRxivpreprint@biorxivpreprint.bsky.social · 8mo ago

The vault associates with membranes in situ https://www.biorxiv.org/content/10.64898/2025.12.12.693869v1

Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...

Fantastic new work from our researchers! Check out their work on 🐯 cryoTIGER for cryoET 🐯

Jan Philipp Kreysing@jpkreysing.bsky.social · 10mo ago

❓What if you could peek between your tilt series ❓ We are proud to introduce cryoTIGER -- deep-learning based tilt interpolation for enhanced reconstruction in cryo-ET. A great collaboration between Turoňová lab, @becklab.bsky.social, @hummerlab.bsky.social #teamtomo www.nature.com/articles/s42...

New from my colleagues Markus Schreiber & Beata Turoňová: TANGO, an #opensource tool for more holistic spatial analysis of cryo-ET particles. It can clean or cluster particles, analyze lattices, and identify patterns. #TeamTomo www.biorxiv.org/content/10.1...

Twist and Scout: Analysis and Curation of Particles in Cryo-Electron Tomography Using TANGO

Cryo-electron tomography (cryo-ET) enables the visualization of cellular structures in near-native environments, but its potential for spatial analysis has been underutilized due to a lack of versatil...

biorxiv.org

Excited and honored to speak at the @royalmicrosoc.bsky.social meeting tomorrow where I'll talk about our latest AI tools. Meanwhile, check out Vijay&Arlo's Napari "bootstrapper" plugin for massively accelerated segmentation of multidimensional imaging datasets Github: github.com/ucsdmanorlab...

In situ evidence for systematic membrane thickness variation across cellular organelles https://www.biorxiv.org/content/10.1101/2025.03.13.642912v1

Automated Removal of Corrupted Tilts in Cryo-Electron Tomography https://www.biorxiv.org/content/10.1101/2025.03.13.642992v1