thrilled to share that my main phd project is finally out in @natcomms.nature.com we present high-resolution structures of RyR1 in native membranes across multiple functional states by both cryo-ET/StA #TeamTomo and SPA! paper: www.nature.com/articles/s41... and a thread below⬇️⬇️⬇️

Caveolae help cells withstand mechanical stress, take up nutrients, and regulate signaling. How are these tiny membrane structures stabilized? #mdcBerlin researchers have now resolved the structure of the EHD2 protein chain that does the job. Learn how it works: 👉 www.mdc-berlin.de/news/press/h...

Big thanks to @arnemoeller.bsky.social , @djanuliene.bsky.social, all speakers, and everyone who made the #WEHeraeusSeminar - Cryo-EM on the Move such an amazing event! It was a blast! #cryoEM #StructuralBiology #Microscopy

We finally wrote up the paper for LocScale-2.0. edu.nl/pvhxv Please try it out. LocScale is evolving & we are actively building in new features and improvements. Your feedback will help us shape the next steps. Code: cryotud.github.io/locscale/ Work by @alok-bharadwaj.bsky.social and Reinier.

Arjen Jakobi@cryotud.bsky.social · last yr.

We are happy to release LocScale2.0: a tool for context-aware, confidence-weighted cryoEM map optimisation. Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation. cryotud.github.io/locscale/

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

bioRxiv Biophysics@biorxiv-biophys.bsky.social · last yr.

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles https://www.biorxiv.org/content/10.1101/2025.09.08.674935v1

i've just submitted 60 maps to the EMDB 😅 wonder how many validation reports should now be sent in with the MS, askin for a friend...

Building atomic models to cryo-EM maps of huge molecules is intimidating: crowded, uncertain copy numbers, variable resolution ... Fan Liu and team incl us, show how cross-linking MS can be used to build novel models of unknown complexes, like a giant Melbourne virus biorxiv.org/content/10.1...

Happy to have been able to contribute to Lars‘ work!

bioRxiv Biochemistry@biorxiv-biochem.bsky.social · last yr.

Integrative structural interactomics reveals protein organization and structure in a giant virus https://www.biorxiv.org/content/10.1101/2025.06.16.659922v1

We have a preprint for you EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...