Our work expanding the Large Serine Integrase toolkit is finally out! We used alphafold-multimer to identify likely recombination directionality factors and tested them experimentally. Much credit to fantastic postdoc Heewhan Shin. @heewhanshin.bsky.social‬ #Alphafold #MobileGeneticElements

Nucleic Acids Research@narjournal.bsky.social · last yr.

⭐NAR Breakthrough!⭐ AlphaFold2 predicted LSI–RDF interactions and experimentally validated several new functional pairs. @phoeberice.bsky.social @femijohn.bsky.social doi.org/10.1093/nar/... #NARBreakthrough #SyntheticBiology #AlphaFold2 #GenomeEditing #ComputationalBiology

Finally posted - our structures that explain directionality control in large serine integrases! Read all about how these amazingly clever molecular swivels are controlled: www.biorxiv.org/content/10.1... Much hard work by postdoc Heewhan Shin and many thanks to collaborator Femi Olorunniji.

Structural basis of directionality control in large serine integrases

Large serine integrases (LSIs) catalyze unidirectional site-specific DNA recombination reactions, yet those reactions are reversed by the presence of a cognate recombination directionality factor (RDF...

biorxiv.org