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Florian Jug@florianjug.bsky.social · 4mo ago

It is finally out there in published form. 🎉 #MicroSplit (with an 𝕊) #Microscopy 🔬 #AI 🤖 www.nature.com/articles/s41... And no paywall... so... enjoy the read! 💸 👋 This is where the post could end... but I will have to go on for a bit below... 😉 🧵

Never noticed before how nice the Harvard chemistry building looks.

First demonstration of event cameras for neuronal voltage imaging. Very exciting to read. www.biorxiv.org/content/10.6...

Absolutely thrilled to be part of this amazing project. Let's keep breaking the frustration pyramid!🥳

Florian Jug@florianjug.bsky.social · 2y ago

Immagine you could image two cellular structures in the same fluorescent channel and still reliably get them separated afterwards… What would you do with this? Now… what would you do if that also worked with 4 structures at once? 👇 #MicroSplit #preview🧵

The first successful use of 2P voltage imaging in vivo with rhodopsin-based GEVI indicators🥳 www.science.org/doi/full/10....

Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators

Voltage sensitivity in FRET-opsin indicators comes from a photocycle intermediate, reachable via optimized 2P excitation.

science.org

Acquiring data :)