Join Dr Stefan Mueller on 20 Aug to see how FCCS on the EI-FLEX Pro HT directly quantifies ternary complex formation, cooperativity and stability for next-generation therapeutics. Register: us06web.zoom.us/meeting/regi... #ternarycomplex #fccs #proteininteractions #drugdiscovery

Surface immobilisation can limit analysis of transient and multi-component interactions. FCCS measures ternary complex formation, affinity and kinetics directly in solution - no immobilisation or binary kinetics required. See our new application note > buff.ly/jvbB3MY #spr #fccs #ternarycomplex

Quantify ternary complex kinetics with FCCS | Exciting Instruments

Discover how FCCS quantifies affinity, cooperativity, and kinetic stability in ternary complexes using dual HER2-binding antibodies.

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Curious about #smFRET data on #ssDNA? Our latest application note from the Antony Lab (Saint Louis University) shows how EI-FLEX data reveals that RPA wraps ssDNA rather than stretching it, resolving a long-standing question. Read more: buff.ly/N0hSA1h #DNA #AppNote

Protein-driven ssDNA conformational control | Exciting Instruments

Discover the linear relationship between FRET efficiency and ssDNA length, and how this can capture protein-mediated conformational control.

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🙌 New preprint from Exciting Instruments! We show how FCCS directly characterises HER2–trastuzumab–pertuzumab ternary complexes in solution, quantifying affinity, cooperativity & dissociation rates without first establishing binary interactions. Read more: excitinginstruments.com/resource-lib...

Protein Ternary Complex Quantification | Exciting Instruments

See how FCCS on the EI-FLEX Pro HT quantifies affinity, cooperativity and kinetic stability in HER2–antibody ternary complexes, supporting multispecific drug discovery.

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If you work in molecular biology, genomics, biophysics, or biotech, this seminar is worth your time. Prof. Taekjip Ha explores how single-molecule FRET is advancing our understanding of molecular machines, CRISPR, and Prime Editing > 📺 youtu.be/w0Idz5AoW4E?... #singlemolecule #biotechnology

Headed to the #BBS & #DGfB conference in Glasgow? Meet @romanrenger.bsky.social to discuss single-molecule FRET, FCS, biomolecular dynamics, and complex formation. Catch his talk Wed 3:30pm, visit our booth or poster (Session 2B), or email roman.renger@excitinginstruments.com

📢 Today's the day! Join Tim Craggs and Prof. Taekjip Ha for this afternoon's Exciting Seminar celebrating 30 years of smFRET. Hear from the pioneer behind the first smFRET measurement and discover what's next. Register: us06web.zoom.us/meeting/regi... #biophysics #singlemoleculeFRET #virtualevent

🔬 Quantify cell-free protein production in real time with FCS - no purification required. Our new application note shows how the EI-FLEX tracked YFP-CopB synthesis over 10 hours and distinguished protein signal from lysate autofluorescence > buff.ly/zp4FsQI

FCS analysis of cell-free protein production | Exciting Instruments

Discover a purification-free method using FCS to characterise the real-time cell-free production of chlamydia outer protein B (CopB).

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📢 Join us on 13 July for a virtual seminar on Conformational control: From single-molecule FRET to novel biotechnologies with our CEO Tim and Prof. Taekjip Ha. 11:00am EDT | 8:00am PDT | 4:00pm BST | 5:00pm CEST Register: us06web.zoom.us/meeting/regi... #smFRET #biophysics #singlemolecule

Struggling to quantify interactions of proteins with binding partners, even in complex environments like the cellular cytoplasm or the cell membrane? Fluorescence correlation spectroscopy (#FCS) could be the answer. Explore practical workflows, applications and FCS/FCCS comparisons: buff.ly/AJl60jr

🎉 30 years of single-molecule FRET! Since Taekjip Ha's landmark paper, smFRET has transformed molecular biology. Celebrate with us and share how smFRET has advanced your research: www.linkedin.com/pulse/30-yea... #smFRET

30 years of single-molecule FRET - a celebration 🎉

Hi there, Single-molecule FRET has been transforming how researchers investigate conformational changes, molecular interactions, and complex biological processes for the past 30 years. Today marks 30 ...

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🙌 Congrats to the Bonilla lab on their new preprint! Streit et al. combined cryo-EM, biochemistry, and smFRET on the EI-FLEX to uncover how flaviviral RNA dynamics guide assembly of the replication-initiation complex. A great example of why dynamics matter 💪 buff.ly/r3OHZrY

smFRET and cryo-EM reveal RNA dynamics | Exciting Instruments

See how smFRET on the EI-FLEX, combined with cryo-EM, revealed how dynamic RNA structures regulate RNA-protein interactions and drive flavivirus replication.

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🎂 As our celebrations of smFRET's 30th birthday continue, here are some key papers from the past three decades. From the first single-molecule study to capturing RNA dynamics, see how smFRET expanded to use alternating laser excitation, 3 colours, and even work in live cells. Papers in the comments.

Some molecules don’t like to sit still. That’s why Karim & Roman are heading to #ESAB2026 in #Montpellier! Visit us at booth 5 to explore #smFRET, #FCS & #FCCS, uncover molecular dynamics and interactions in solution, and see how EI-FLEX brings #single-molecule fluorescence into everyday workflows.

🎉 #smFRET has been transforming how we study molecular interactions and dynamics for 30 years! Join us on 13th July as we celebrate past discoveries, current capabilities, and future frontiers. Featuring Prof. Taekjip Ha, who made the first-ever smFRET measurement. Register: buff.ly/riRgb6b

👋 Hands up who's at #MachinesonGenes this week! Find Roman to learn how our easy-to-use tools allow you to assess the conformational dynamics and interactions of individual molecular machines. In solution, on the benchtop. Don't miss his talk on Tuesday morning (10:20 am). #smFRET #FCS

🐮 What lies between the “spherical cow” of purified proteins and live-cell imaging? The messy middle. Explore single-molecule biophysics in crude lysates and serum, with real-world case studies and practical workflows. excitinginstruments.com/resource-lib... #biophysics #singlemolecule

Embracing biophysics in cell lysates or serum | Exciting Instruments

Explore how the 'messy middle' of biophysics, namely cell lysates and serum, combines single-molecule sensitivity with biological context.

excitinginstruments.com

Antibody screening in hours, not days. LLNL researchers used Fluorescence Correlation Spectroscopy (#FCS) to quantify antibody-antigen binding directly in cell-free lysates with minimal sample consumption - buff.ly/kLhspZX Download our practical FCS handbook here - buff.ly/Dkar7zR #antibodies

Antibody Fragments | Exciting Instruments

Discover EI-FLEX data showcasing how CFPS combined with FCS accelerates the production and characterisation of antibody fragments.

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Running smFRET experiments, but unsure how to label your samples or choose the right dyes? Our technical note covers dye selection, site-specific labelling, and key design considerations to help you get reliable data. 📖 excitinginstruments.com/resource-lib... #smFRET #biophysics #biochemistry #howto

👀 Does the answer to your scientific question depend on understanding how molecules truly interact in solution? 🙌 FCS and FCCS make these measurements possible, at single-molecule sensitivity and without surface immobilisation. 📘 Learn more in our new handbook > buff.ly/CH1uUcm

🙌 Yesterday at #smbio26 was a shining example of cutting-edge single-molecule science in the UK. The team is on hand again today to discuss how our technology can help you see all the Exciting things your molecules are doing. Want a demo of our system at our nearby HQ? Just let the team know.

👀 Crystal structures don’t always reveal the full conformational landscape of proteins. Using smFRET + FCS on the EI-FLEX, Howard et al. uncovered two previously unknown salt-dependent conformational states in the bacterial helicase Rep. Read more: buff.ly/smLjyaG #smFRET #FCS #biochemistry

Who's heading to #smbio26 this week? Catch up with @timcraggs.bsky.social and @romanrenger.bsky.social to discover next-generation single-molecule fluorescence technologies that are helping researchers reveal biomolecular interactions and dynamics with exceptional sensitivity and throughput.

🤔 Is the ‘average’ molecule a liability in modern drug discovery? Bulk assays hide the transient states that decide whether biologics & TPDs succeed. Discover how smFRET, FCS & FCCS can accelerate drug discovery > buff.ly/fEfiuJM

🔎 How do antiviral therapeutics work at the molecular level Using smFRET + FCS, Groves et al. investigated how remdesivir and suramin inhibit SARS-CoV-2 RdRp, revealing distinct mechanisms of action beyond gel-based assays. 👀 excitinginstruments.com/resource-lib...

🇺🇸 Heading to the Industrial Biostructures America #ProterosIBAConference next week? Visit Jake at our booth for an intro to the EI-FLEX Pro, enabling biopharma labs to distinguish individual conformers in solution and quantify biomolecular heterogeneity with ease. #drugdiscovery #biopharma

🤔 Still wondering how smFRET works in practice - and how it complements your current techniques? Our handbook shows when and why to use it alongside existing methods to add dynamic, single-molecule insight. Download: buff.ly/CxuqL1A #smFRET #biophysics #biochemistry

👋 Run a core facility with cryo-EM or X-ray crystallography? Add smFRET/FCS to reveal dynamics, heterogeneity & interactions in solution - helping your users link structure to function with the EI-FLEX system. excitinginstruments.com/ei-flex/?utm... #biophysics #biochemistry #corefacility

Structures show what #proteins look like - but not how they move. Learn how #smFRET adds dynamics to X-ray, Cryo-EM & AlphaFold to reveal allostery, flexibility & cryptic sites. 👀 buff.ly/c9Bijmx

🔬 Studying #protein-DNAinteractions and wondering how freely-diffusing #single-molecule techniques can support your research? Here are a couple of the most commonly asked questions we hear from scientists in this space. Find more on our protein-DNA application page > buff.ly/uOHzEy5