Poly-ubiquitylated proteins proved trickier to purify for LC-MS than expected. Non-specific background swamped isolations: with histones and uncertainty! This led us to develop Ubi-SCAPE—for characterising the poly-ubiquitylome to unprecedented purity and depth. www.biorxiv.org/content/10.6...
Harvey Johnston
@harveyejohnston.bsky.social
Proteomics Facility Co-Manager at The Cambridge Institute for Medical Research. Collector of obscure proteomics acronyms... Founder of LPDG and CamsPaMS. Developer of SP4 and Ubi-SCAPE.
The 2nd Cambridge Proteomics and Mass Spectrometry meeting is <2 weeks away: 12-5pm Thurs 3rd Apr @ Babraham (networking 11am-pub) 5 talks, 5 networking sessions, a ‘methods challenge’, up to 24 posters, free lunch+dinner, + up to 200 scientists! To register please visit: www.CamsPaMS.org
Registration for the 2nd Cambridge (UK) Proteomics and Mass Spec meeting is now open! Join us on Thurs 3rd April at the Babraham Research Campus for more talks, networking, posters, and discussions For more details, to register, or to suggest a talk/sponsor see our new website: CamsPaMS.org
Pleased to use my first 🦋 post to thank @mtrost.bsky.social and @drandyfrey.bsky.social for hosting a BBSRC CONNECT FTMA placement sharing proteomics & MS knowledge between @babrahaminst.bsky.social & @newcastleuni.bsky.social Been here <20h and already got some exciting ubiquitin proteomics data!