Sulfadiazine selection enables high‐efficiency transformation in <fi>Nannochloropsis</fi> and <fi>Microchloropsis</fi>

Genetic engineering in the marine eustigmatophytes Nannochloropsis and Microchloropsis is constrained by intrinsic antibiotic resistance and reduced marker efficacy at high salinity. We show that mit....

onlinelibrary.wiley.com

volkerlipka.bsky.xn--socials-d36c

Horizontal acquisition of nicotine catabolism gene cluster enhances Arthrobacter fitness within tobacco root microbiota - Microbiome

Background Plant roots are hotspots for interactions with soil microbes, where a characteristic bacterial community structure is formed. Plant specialized metabolites often play pivotal roles in this assembly process. However, the molecular basis underlying root microbiota responses to these bioactive compounds, and how such metabolic interactions shape the assembly of host-specific root microbiota, remain largely unknown. Nicotine is a toxic alkaloid predominantly produced by the genus Nicotiana, and the genus Arthrobacter is known as one of the nicotine-degrading bacteria in the tobacco root microbiota. In this study, we used the tobacco–Arthrobacter interaction system as a model and integrated comparative genomics and experimental genetic manipulation assays to uncover the role of bacterial catabolism capacity for host specialized metabolites in shaping host-specific root microbiota. Results Nicotine catabolism genes are uniquely found in the Arthrobacter strains derived from nicotine-containing environments, and this restricted gene distribution is driven by a plasmid-mediated horizontal gene transfer. To assess the ecological consequences of this genomic adaptation in Arthrobacter fitness in tobacco roots, we characterized the nicotine utilization ability of Arthrobacter and conducted adaptation assays under in planta conditions using genetically manipulated Arthrobacter strains and tobacco mutants impaired in nicotine catabolism and biosynthesis, respectively. Nicotine improves Arthrobacter colonization of tobacco roots through a catabolism-dependent mechanism. Bacterial community analysis using a synthetic community approach further demonstrated that this metabolic adaptation enhances Arthrobacter fitness within tobacco root microbiota. Conclusions Our findings illustrated that bacterial catabolic capacity toward host-derived plant specialized metabolites is key for successful root colonization. This metabolic adaptation is driven by plasmid-mediated horizontal gene transfer and ultimately shapes the structure of the root microbiota community. Video Abstract

doi.org

Generation of the Camptothecin Scaffold by a Flavin‐Catalyzed Photooxidative Skeletal Reorganization

The biosynthetic pathway of the anticancer drug precursor camptothecin involves an enigmatic skeletal reorganization from a 6/5/6 tetrahydro-β-carboline to a 6/6/5 pyrroloquinoline ring system. Here,...

onlinelibrary.wiley.com

Professur (W3) für Pflanze-Boden-Interaktionen - Universität Hohenheim

Universität Hohenheim bietet Stelle als Professur (W3) für Pflanze-Boden-Interaktionen in Stuttgart - jetzt bewerben!

academics.de

Untangling Inositol (Pyro)Phosphate Biology Through Emerging Technologies

Inositol (pyro)phosphates represent a vital class of intracellular messengers that govern multiple biological processes. The last decade has experienced a surge of interest in the functions of this c...

advanced.onlinelibrary.wiley.com

Bifurcation of StCRY1-StHY5 axis orchestrates blue light-enhanced glycoalkaloid and chlorophyll accumulation in potato tubers

Abstract. Potato, the fourth largest food crop in the world, stores nutrients in underground tubers. However, light exposure induces tuber accumulation of

academic.oup.com

Igor Levit und Danger Dan: ZDF-Geschäftsleitung untersagt Auftritt der Musiker

Eigentlich sollten der Pianist Igor Levit und der Rapper Danger Dan in der Satiresendung »Die Anstalt« ihren neuen Song spielen. Nun schreitet die ZDF-Intendanz ein. Die Musiker sprechen von einem »au...

spiegel.de

Stellenanzeigen - Georg-August-Universität Göttingen

Webseiten der Georg-August-Universität Göttingen

uni-goettingen.de

Rapid DNA extraction from faba bean (Vicia faba) tissues

Rapid DNA extraction from faba bean (Vicia faba) tissues. Efficient DNA isolation is essential for hig. Read full protocol, steps, and materials on protocols.io

protocols.io

Think preprints are unreliable? Analysis of 70,000 studies might change your mind

Preprints don’t change much after peer review — and are rarely retracted.

go.nature.com

Hannover: Polizei ermittelt nach »Fridays for Future«-Demo wegen Volksverhetzung

Bei einer Demonstration von Klimaschützern in Hannover sollen Teilnehmende antisemitische Parolen skandiert und Beamte angegriffen haben. Die Polizei ermittelt.

spiegel.de

KI-generiertes Plakat: Wenn eine Schule plötzlich judenfeindliche Klischees verbreitet - WELT

Antisemitische Bildsprache auf einer Einladung zum Schultheater: Eine Werbung für „Der Geizhals“ in Freudenstadt zeigt eine geldgierige Figur mit riesiger Hakennase. Die Schule löscht das Bild – und v...

welt.de

A catastrophic marine mortality event caused by a complex algal bloom including the brevetoxin producer Karenia cristata

Nature Ecology & Evolution - Genomic characterization and toxicity assays identify Karenia cristata as a brevetoxin-producing species that is the dominant component of the prolonged microalgal...

rdcu.be

Bacteria sense virus-induced genome degradation via methylated mononucleotides

Phages often degrade the genome of their bacterial host to individual nucleotides. Here we describe Metis, a bacterial defense system that directly senses phage-mediated host genome degradation. Metis...

science.org

Protein Turnover in Plants – Cost of Synthesis, Repair and Crop Engineering Opportunities

Abstract. Protein lifespan is shaped by plant developmental stage and cellular requirements; both are fairly well understood processes. Beyond regulated de

academic.oup.com

sciencedirect.com

The Novo Nordisk Foundation Prize

Our prizes are awarded to researchers who have contributed to scientific breakthroughs or in other ways distinguished themselves within natural or medical science.

novonordiskfoundationprize.com

Cephalotaxinone enzymes reveal a whole plant model for homoharringtonine biosynthesis - Nature Chemical Biology

Homoharringtonine (HHT) is a plant-derived anticancer drug approved by the US Food and Drug Administration. Here, the authors elucidate a root-tip-specific biosynthetic pathway to its alkaloid core, i...

nature.com