A bit dense, but the important message is dead simple: We got to cite the E = mc² paper! Kidding :) The important part is that combinations of isotopes unlock 100-1000x gains in protein measurement speed. Here's how: www.biorxiv.org/content/10.1... #TeamMassSpec #Proteomics 🧪

How to design 1000-plex mass tags using the differential mass defect

Multiplexing samples in mass spectrometry-based proteomics has long been accomplished by isotopologues of small molecules. These chemically-identical "mass tags" conjugate to peptides to encode sample...

biorxiv.org

The hits keep on coming from @parallelsq.bsky.social! Today we released an exciting use-case for plexDIA in single cell analysis of #alzheimer's disease, characterizing dysregulation of the 20S proteasome. #TeamMassSpec #Proteomics www.biorxiv.org/content/10.1...

Ubiquitin-Proteasome System Dysregulation in Alzheimer's Disease Impacts Protein Abundance

Alzheimer's disease (AD) is a relentlessly progressive, fatal neurodegenerative disorder associated with widespread aberrant proteomic changes. The full extent of protein dysfunctions in AD and their ...

biorxiv.org

Members of PTI have developed mass tags which improve spectral quality and enable 9-plexDIA in a project lead by @harrisons.bsky.social

Harrison Specht@harrisons.bsky.social · last yr.

🧪 Transforming biomedical discovery needs proteomics that is specific and accurate, but also faster and cheaper @parallelsq.bsky.social is proud to introduce 9-plex PSMtags: a new mass tag that improves sequencing and increases throughput in sensitive proteomics 👇 www.biorxiv.org/content/10.1...

We are excited to introduce ‘time’ as a new domain for proteomics multiplexing! It enables: -Label-free multiplexing -Combinatorial multiplexing with plexDIA Using combined 9-plexDIA and 3-timePlex we demonstrate 27-plex DIA 🚀