Mapping transcriptional responses to cellular perturbation dictionaries with RNA fingerprinting

Single-cell perturbation dictionaries provide systematic measurements of how cells respond to genetic and chemical perturbations, and create the opportunity to assign causal interpretations to observa...

biorxiv.org

Iterative, multimodal, and scalable single-cell profiling for discovery and characterization of signaling regulators

Cell signaling plays a critical role in regulating cellular state, yet uncovering regulators of signaling pathways and understanding their molecular consequences remains challenging. Here, we present ...

biorxiv.org

mTORC1 activation drives astrocyte reactivity in cortical tubers and brain organoid models of TSC

Tuberous Sclerosis Complex (TSC) is a genetic neurodevelopmental disorder associated with early onset epilepsy, intellectual disability and neuropsychiatric disorders. A hallmark of the disorder is co...

biorxiv.org

Phospho-seq: integrated, multi-modal profiling of intracellular protein dynamics in single cells - Nature Communications

Here, the authors demonstrate Phospho-seq, a single-cell multiomics method capable of quantifying chromatin accessibility alongside intracellular proteins, including post-translationally modified prot...

nature.com