I wonder if @nanoporetech.com will ever release the much faster native barcoding kit where the sequencing adapters simply have the barcodes included. Surely those 75 minutes extra could save a life one day (+ minimize DNA loss and lab frustrations)
Rasmus Kirkegaard
@kirk3gaard.bsky.social
Staff scientist having fun with DNA seq and bioinformatics at #AlbertsenLAB
New blog post! I analyse the new hac@v6.0.0 basecalling model from @nanoporetech.com and discuss the conspicuous lack of a new sup model: rrwick.github.io/2026/06/11/d...
Dorado v2.0.0: no more sup?
a blog for miscellaneous bioinformatics stuff
rrwick.github.io
Is @nanoporetech.com hac v6 better than 5.2.0 sup? The short answer is no. But are a few errors in a genome worth 5 times more basecalling compute? github.com/Kirk3gaard/M...
Without trusted references, methylation data is hard to interpret. Using ATCC Genome Portal resources and nanopore sequencing, this webinar shows how to turn raw microbial modification signals into biological insight. https://bit.ly/4o369uU
It's also a boon for service providers with proms and high throughput. Many users can't/don't rebasecall and running sup on-machine limited the # of flow cells that could be run. v6 hac model means that more flow cells can be run concurrently to provide Q23 data directly for clients to run with.
Hac 6.0.0 seems like a mixed bag to me compared to sup 5.2.0 based on github.com/Kirk3gaard/M... . Thanks for your hard work @kirk3gaard.bsky.social
github.com
Thought i'd highlight that the ONT London Calling tech talk is now up. Points of interest for the microbiome community: 1) Direct RNA multiplexing now available. Can now run 24 samples per flow cell, recover full-length transcripts with 8 base pair modifications... www.youtube.com/watch?v=CE69...
London Calling 2026 Technology update
YouTube video by Oxford Nanopore Technologies
youtube.com
Jeremy Wang developed rammap, a minimap2 rewrite in Rust. It achieves comparable or better performance than minimap2 and produces identical output to minimap2. During rewrite, Jeremy found two long-existing bugs in minimap2 which are fixed in v2.31. www.biorxiv.org/content/10.6...
biorxiv.org
For many years you needed UMIs and similar dark molecular biology magic to get perfect amplicon sequences from @nanoporetech.com data. With recent accuracy improvements+really clever algorithms that is now changed. Savont unlocks ASVs from low coverage nanopore amplicon data 🤯🤯🤯
Jim Shaw@jimshaw.bsky.social · 2mo agoOur method, savont, for generating amplicon sequence variants (ASVs) for long-read amplicons is now on bioRxiv. Work with @lh3lh3.bsky.social and help from @mkddueholm.bsky.social and team (Marie Riisgaard-Jensen, @kirk3gaard.bsky.social, Kasper Skytte Andersen) github.com/bluenote-157... 1/6
Abstract submissions for the T2T Face-to-Face conference are open through June 15th! Join the community Sept. 3–4 in Santa Cruz, CA, for two days of discussion and collaboration around telomere-to-telomere genomics and pangenomics. Learn more: sites.google.com/ucsc.edu/t2t...
New preprint from us! 🦠 Clinical metagenomics is great for sequencing pathogens directly from patient samples but for some protocols sensitivity for RNA viruses lags behind bacteria & fungi. We have shown why, and how to fix it. www.medrxiv.org/content/10.6...
Library preparation strategy critically impacts RNA virus sensitivity in clinical metagenomics
Clinical metagenomics uses sequencing for culture-independent identification of pathogens directly from clinical specimens. While a number of protocols claim to be pathogen agnostic, sensitivity for R...
medrxiv.org
On this day in 2015 a disruptive company handed out DNA sequencers in goodie bags 🤯🤯🤯. Next week @nanoporetech.com is hosting their yearly London Calling event. Bring back disruption 🧬🚀
The nvidia dgx spark is a cool box but you get more @nanoporetech.com basecalling for your money with one of the other options (github.com/Kirk3gaard/2...)
Ever wondered what microbes you could find in Denmark? Pupils from Danish schools collected soil samples across the country so you can now browse the microbes (cmc-aau.github.io/masseeksperi...).
Performance Test of the QNome Nanopore Sequencer https://www.biorxiv.org/content/10.64898/2026.04.29.721586v1
Similar situation in academia when the professors "win money" to the university 🤣
The Onion@theonion.com · 3mo agoRacehorse Receives Carrot Every Time He Wins $2 Million For Owner
Anyone happen to have a dgx spark doing nothing? Benchmark it for @nanoporetech.com basecalling (github.com/Kirk3gaard/2...)
More do it at home @nanoporetech.com sequencing is being announced on X (x.com/SethSHowes/s...) A blog post by Seth Howes (iwantosequencemygenomeathome.com). Hope London Calling will feature a tinkerer session this year.
x.com
There's a reason why most undiscovered taxa come from soils, sediments, wetlands, etc: www.nature.com/articles/s41... The way I prefer to think about it is to put that money into long reads and get usable data.
Unbinned contigs expand known diversity in the global microbiome - Nature Microbiology
Re-analysis of over 92,000 metagenomes reveals hundreds of thousands of previously undescribed Bacterial and Archaeal clades hidden in plain sight.
nature.com
A major milestone for biodiversity genomics: EBP-affiliated projects have now contributed over 6,000 genome assemblies toward the goal of sequencing all known eukaryotic life. 🧬 Thank you to all EBP-affiliated projects behind this work, from sampling and DNA extraction to sequencing and assembly.
Our ECE-host framework doesn't care which sequencing platform you use—it’s ready to go once you have mod calling results. The kicker is that we need scalable mod calling from metagenomics. If it doesn't scale, the ECE-host analysis can't happen.
Congratulations @wshuai.bsky.social and co-authors! We’re excited to introduce MODIFI, our new scalable method for detecting DNA modifications in PacBio metagenomic data and estimating ECE-host linkage. Check out the preprint: www.biorxiv.org/content/10.6...
biorxiv.org
videocardz.com/newz/hp-unve... wow, 4 Nvidia RTX Pro 6000 Blackwell GPU support. Future Oxford Nanopore attachment for the P48 sequence? @kirk3gaard.bsky.social
HP unveils Z8 Fury G6i workstation with quad RTX PRO 6000 Blackwell GPUs offering 384GB VRAM - VideoCardz.com
HP Z8 Fury G6i supports up to four RTX PRO 6000 Blackwell GPUs HP has introduced the Z8 Fury G6i, a new high-end workstation built around Intel’s Xeon 600
videocardz.com
_720 Gbp_ marine nanopore metagenome -> 328 circular prokaryotic contigs: using myloasm! Insane work by Lui and Nielsen. Also shows how modern long read assemblies can disentangle coexisting strains and reveal ecological insights.
bioRxiv Microbiology@biorxiv-microbiol.bsky.social · 4mo agoContrasting population structures coexist in a strain-resolved estuarine microbiome https://www.biorxiv.org/content/10.64898/2026.03.20.713316v1
Will 2026 be the year we finally see something new from @nanoporetech.com or are we stuck due to the clinical focus? Next generation ASIC chips would be the opportunity to align product lines. One flowcell to rule them all?
A new era for #ONT amplicon sequencing? We show that #ONT #amplicon sequencing now achieves accuracy sufficient for #ASV resolution using standard Illumina-based pipelines. We validated this by sequencing identical amplicons on #ONT and #PacBio. @nanoporetech.com www.biorxiv.org/content/10.6...
