Kai Johnsson
@kjohnsson.bsky.social
Chemical biologist @ Max Planck Institut for Medical Research in Heidelberg
Localizable fluorescent metal ion indicators galore: advanced.onlinelibrary.wiley.com/doi/10.1002/...
Localizable Fluorescent Metal Ion Indicators With Tunable Colors
A modular platform enables the generation of fluorescent metal ion indicators with tunable emission colors and precise subcellular localization. Conjugation to self-labeling proteins yields bright, c...
advanced.onlinelibrary.wiley.com
A high-affinity split-HaloTag for live-cell protein labeling. And much more. www.nature.com/articles/s41...
A high-affinity split-HaloTag for live-cell protein labeling
Nature Communications - Lin and colleagues present high-affinity split-HaloTag pairs for protein tagging and multiplexed labelling. This versatile system allows protein visualisation with diverse...
nature.com
A Cinderella story: raising CLIP-tag from the ashes to shine in fluorescence labeling. www.biorxiv.org/content/10.6...
biorxiv.org
A good end to 2025: rhodamine-binders for bioimaging in JACS: pubs.acs.org/doi/10.1021/...
Fast, Bright, and Reversible Fluorescent Labeling of Rhodamine-Binding Proteins
Rhodamine dyes conjugated to targeting ligands can yield exceptionally bright fluorescent probes for live-cell imaging. However, the limited permeability of such rhodamine derivatives restricts their ...
pubs.acs.org
Molecular recording of cellular protein kinase activity with chemical labeling: www.nature.com/articles/s41... Congratulations to De-en Sun and the entire team.
Molecular recording of cellular protein kinase activity with chemical labeling - Nature Chemical Biology
Molecular recorders based on kinase activity-dependent protein labeling track specific kinase activities to understand their link to cellular phenotypes in heterogeneous cell populations and in vivo.
nature.com
Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging: www.biorxiv.org/content/10.1... Congratulations to Julian Kompa and the entire team.
Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging
We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...
biorxiv.org
Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
nature.com
Please check out our new high-affinity split-HaloTag for live-cell protein labeling www.biorxiv.org/content/10.1... Congratulations to Yin-Hsi Lin!
biorxiv.org
Improving split-HaloTag through computational protein engineering: onlinelibrary.wiley.com/doi/10.1002/...
Improving split‐HaloTag through computational protein engineering
Split-HaloTag can be used to transform transient molecular interactions into permanent marks through chemical labeling, thereby enabling the recording of transient physiological events in individual ....
onlinelibrary.wiley.com
Check out our new fluorescent probe for imaging f-actin dynamics: 𝗦𝗶𝗥-𝗫𝗔𝗰𝘁𝗶𝗻: 𝗔 𝗳𝗹𝘂𝗼𝗿𝗲𝘀𝗰𝗲𝗻𝘁 𝗽𝗿𝗼𝗯𝗲 𝗳𝗼𝗿 𝗶𝗺𝗮𝗴𝗶𝗻𝗴 𝗮𝗰𝘁𝗶𝗻 𝗱𝘆𝗻𝗮𝗺𝗶𝗰𝘀 𝗶𝗻 𝗹𝗶𝘃𝗲 𝗰𝗲𝗹𝗹𝘀 www.biorxiv.org/content/10.1... Thank you @veselin-nasufovic.bsky.social
SiR-XActin: A fluorescent probe for imaging actin dynamics in live cells
Imaging actin-dependent processes in live cells is important for understanding numerous biological processes. However, currently used natural-product based fluorescent probes for actin filaments affec...
biorxiv.org