🎉 Minli Ruan’s new paper shows how PUS7 localization rewires mRNA pseudouridylation, reshaping the proteome and influencing cellular fitness under ROS in yeast and human cells. Huge thanks to our collaborators in the Contreras, Jain, and Rouhanifard labs!

Cytoplasmic localization of pseudouridine synthase 7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance - Nature Communications

Pseudouridine is a widespread RNA modification implicated in the regulation of gene expression. Here, authors show that PUS7 relocalization changes mRNA pseudouridylation, reshapes the proteome, and e...

doi.org

It’s preprint week in the Koutmou lab! With Lyle Simmons’ lab, we identify rRNA methyltransferases in a Gram-positive bacterium, assign functions to 11 uncharacterized B. subtilis genes, and show how chemical modifications shape ribosome assembly and antibiotic sensitivity. doi.org/10.64898/202...

New preprint from Kaley Simcox in the Koutmou lab! She developed a HILIC-MS/MS approach for total tRNA sequencing, achieving 75–100% coverage for most isoacceptors - without ion-pairing reagents. This enabled the discovery of new modified positions on E. coli tRNAs. doi.org/10.64898/202...

New preprint from the Koutmou, Jain & Wiedenheft labs! We identify sub-stoichiometric pseudoU sites in MS2 RNA genome and benchmark Nanopore DRS with LC-MS/MS + CLAP. Takeaway: broad mod trends can inform biology, but individual calls/occupancy need orthogonal validation. doi.org/10.64898/202...

Excited to announce a pre-print about how endogenous mRNAs pseudouridylation can impact translation and cellular fitness: doi.org/10.1101/2025.09.22.675887 Congrats to PhD student Minli Ruan, who spearheaded this *highly collaborative* project with the Contreras, Jain and Rouhanifard labs.

A Puerto Rican Ph.D. student. A first-gen scientist. Both lost NIH grants under vague “new priorities.” Nearly 2,500 projects and $1.6B in cut or stalled grants. No vote. No debate. Just science erased. www.nytimes.com/interactive/...

Here Are the Nearly 2,500 Medical Research Grants Canceled or Delayed by Trump

Some cuts have been starkly visible, but the country’s medical grant-making machinery has also radically transformed outside the public eye.

nytimes.com