Laina Hall
@lainahall.bsky.social
Graduate Student in the Lucas lab at UC Berkeley
New preprint from the lab! Led by graduate student @lainahall.bsky.social we show a new method to thin cells for cryo-EM imaging with minimal damage. www.biorxiv.org/content/10.6...
Optimized cryo-FIB milling strategy to generate thin, minimally damaged biological lamellae
Focused ion beam (FIB)-milling has been adapted to thin frozen cells for visualization of macromolecular structures in situ with cryogenic electron microscopy. However, only a few large and abundant c...
biorxiv.org
With Cryo-ET/EM, the macromolecules we study (and biological questions we ask) are SNR-limited. Here, we share Nilas, a low-voltage milling method named after thin sea ice. Nilas produces thin (sub 50 nm in places!), minimally damaged lamellae. biorxiv.org/content/10.64898/2026.07.21.739890v1
Optimized cryo-FIB milling strategy to generate thin, minimally damaged biological lamellae
Focused ion beam (FIB)-milling has been adapted to thin frozen cells for visualization of macromolecular structures in situ with cryogenic electron microscopy. However, only a few large and abundant c...
biorxiv.org
Now out in full form. Again, a big thanks to #teamtomo and @alisterburt.bsky.social for developing the infrastructure to build this on. journals.iucr.org/d/issues/202...
Leopard-EM: an extensible 2D template-matching package to accelerate in situ structural biology
In situ cryo-EM coupled with 2D template matching (2DTM) holds the potential to visualize the cellular structureome in context, but further developments are required to make this a reality. We describ...
journals.iucr.org
First pre-print from the Lucas Lab led by Matthew Giammar and @joshdcryoem.bsky.social online today! We develop a new, extensible Python implementation of 2DTM and apply it to build pixel size optimization, constrained search and characterize molecular motions in situ www.biorxiv.org/content/10.1...
Leopard-EM: An extensible 2DTM package to accelerate in situ structural biology
The ability to generate high-resolution views of cells with cryogenic electron microscopy (cryo-EM) can reveal the molecular mechanisms of biological processes in their native cellular context. The re...
biorxiv.org
PhD student @lainahall.bsky.social will be giving a talk about our measurements of FIB-milling damage in cryo-lamellae (with new data!) at M&M on Tuesday next week.
Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...
To tilt or not to tilt? Strategies for in situ cryo-EM data collection
Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…
sciencedirect.com
New research from IGI Investigator Dave Savage and first author Noam Prywes details the landscape of possible Rubisco variants, suggesting ways to make the world's most abundant enzyme better at its job. 🌱 Read more: ow.ly/t2JO50ULh10
Re-upping this opportunity while the application window is still open!
Jim Hurley@jimhurley.bsky.social · 2y agoScientific Director, Cal-Cryo. We are looking for an outstanding microscopist to run our cryo-EM facility (two Krioses and an Arctica), increase training, engagement, and collaboration on campus, expand our user base, and join our community of cutting edge cryo-EM researchers. tinyurl.com/ykasna3p