International Conference on Hydrogenase and Other Redox Metalloenzymes 2026 | School of Chemistry | University of Leicester

Web page for the International Conference on Hydrogenase and Other Redox Metalloenzymes. The conference will be held in Leicester in Summer 2026.

le.ac.uk

Engineering the Electron Relay in [FeFe]-Hydrogenase Enhances Electrocatalytic H2 Evolution

H2 is an ideal energy vector, but catalysts for its clean production from water are inefficient or expensive. [FeFe]-hydrogenases are the most active H2-converting catalysts in nature, using a unique ...

pubs.acs.org

The Allosteric Regulator Inositol Phosphate Dramatically Affects the Efficacy and Selectivity of Inhibitors for Different HDAC Complexes

Class I histone deacetylases regulate gene transcription and are established therapeutic targets. HDAC1–3 form the catalytic subunit in several distinct multiprotein complexes; however, HDAC inhibitor...

pubs.acs.org

Solvent Channels and Electric Fields Guide Proton Delivery to the Active Site of Heme Peroxidases

Electrostatic potential surfaces and electric field lines reveal that local fields guide proton delivery through multiple conserved solvent channels in heme peroxidases, enabling efficient access to ....

onlinelibrary.wiley.com

Engineering the TCA cycle regulator GarA to increase erythromycin production in Saccharopolyspora erythraea

Actinobacteria are important for industrial production of antibiotics, fine chemicals and food and a source of new compounds for drug discovery. Their central metabolism is regulated by a conserved pr...

microbiologyresearch.org

mdpi.com

Two-dimensional infrared spectroscopy as a tool to reveal the vibrational and molecular structure of [FeFe] hydrogenases

[FeFe] hydrogenases are Nature's most efficient catalysts for the cleavage and evolution of molecular hydrogen. Despite decades of research, key aspects of the catalytic cycle and the underlying geome...

pubs.rsc.org

Leicester Institute for Structural and Chemical Biology | LinkedIn

Leicester Institute for Structural and Chemical Biology | 51 followers on LinkedIn. Our researchers in chemistry and biology are collaborating to improve our understanding of the workings of life. | T...

linkedin.com

Engineered Receptor Capture Combined with Mass Spectrometry Enables High-Throughput Detection and Quantitation of SARS-CoV-2 Spike Protein

Mass spectrometry (MS) is a potentially powerful approach for the diagnostic detection of SARS-CoV-2 and other viruses. However, MS detection is compromised when viral antigens are present at low conc...

pubs.acs.org

Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ - Nature Communications

The molecular basis for the enrollment of X family DNA polymerases in non-homologous end joining (NHEJ) is unclear. Here the authors elucidate the structure of Pol λ within the DNA-PK long-range compl...

nature.com

University of Leicester academic among recipients of prestigious Academy of Medical Sciences Springboard Award | News | University of Leicester

le.ac.uk

Terminal hydride formation in [FeFe] hydrogenase: understanding the role of the dithiolate bridgehead

[FeFe]-hydrogenases are highly-active hydrogen-conversion biocatalysts using Earth-abundant metals in their active-site. Understanding their mechanism may enable design of catalysts for renewable ener...

pubs.rsc.org

Breakthrough molecular movie reveals DNA’s unzipping mechanism with implications for viral and cancer treatments | News | University of Leicester

le.ac.uk

Identification and inhibition of PIN1-NRF2 protein–protein interactions through computational and biophysical approaches - Scientific Reports

Scientific Reports - Identification and inhibition of PIN1-NRF2 protein–protein interactions through computational and biophysical approaches

nature.com

Wellcome awards Leicester scientist £2m to study gene regulation in early development | News | University of Leicester

le.ac.uk

Circular Engineered Sortase for Interrogating Histone H3 in Chromatin

Reversible modification of the histone H3 N-terminal tail is critical in regulating the chromatin structure, gene expression, and cell states, while its dysregulation contributes to disease pathogenesis. Understanding the crosstalk between H3 tail modifications in nucleosomes constitutes a central challenge in epigenetics. Here, we describe an engineered sortase transpeptidase, cW11, that displays highly favorable properties for introducing scarless H3 tails onto nucleosomes. This approach significantly accelerates the production of both symmetrically and asymmetrically modified nucleosomes. We demonstrate the utility of asymmetrically modified nucleosomes produced in this way in dissecting the impact of multiple modifications on eraser enzyme processing and molecular recognition by a reader protein. Moreover, we show that cW11 sortase is very effective at cutting and tagging histone H3 tails from endogenous histones, facilitating multiplex “cut-and-paste” middle-down proteomics with tandem mass tags. This cut-and-paste proteomics approach permits the quantitative analysis of histone H3 modification crosstalk after treatment with different histone deacetylase inhibitors. We propose that these chemoenzymatic tail isolation and modification strategies made possible with cW11 sortase will broadly power epigenetic discovery and therapeutic development.

pubs.acs.org

Research opportunities | Institute for Structural and Chemical Biology | University of Leicester

le.ac.uk