One of the great highlights of the #VoM2026 was a lifetime achievement award to Dr. Ian Molineux at UT Austin, a long-time friend and collaborator of the CPT. Congrats Ian!
Center for Phage Technology
@phagetechnology.bsky.social
Located at Texas A&M University. Devoted to the study of bacteriophages for human health and agriculture. Home of the CPT Galaxy/Apollo phage annotation system. Cpt.tamu.edu
We have two more posters at #VOM2026 today and tomorrow: Poster #119 (July 8) on a versitile and novel phage technology: "Phage based programmable protein overproduction and delivery", and Poster #48 (July 9): "Bacterial anti-phage defense systems in the Burkholderia cepacia complex"
Phages are cubist (a tea room in Prague)
We are presenting two posters today at #VOM2026 if you are around and interested: Poster #198: Understanding infection initiation in Burkholderia cenocepacia phage BcepIL02, a Lessievirus encoding multiple tail fibers, and Poster 209: The role of the sim locus in phage P1 superinfection exclusion
Check out our most recent publication offering new perspectives on the synergy between phage and antibiotcs. We revealed phage-induced cell permeabilization during DNA entry which leads to increased cell vulnerability to antibiotic molecules.
Zihao Yu@yu-zihao.bsky.social · 2mo agoThrilled to share our latest paper on phage-induced permeabilization, now published in Nature Communications! It’s been a long journey, and we’re excited to finally share it. Grateful to all co-authors and everyone who contributed! www.nature.com/articles/s41...
Our newest publication in collaboration with @tubsmedbiotech.bsky.social @tu-braunschweig.de on S. equi immunogenic surface proteins identified by ORFeome phage display journals.asm.org/doi/10.1128/...
Immunogenic Streptococcus equi cell surface proteins identified by ORFeome phage display | mSphere
This work utilized an ORFeome phage display platform to systematically identify antigenic epitopes produced by Streptococcus equi subspecies equi (S. equi), an important equine pathogen and the causative agent of horses strangles. Three major S. equi surface proteins were identified: a novel proline-rich repeat domain protein, a serine peptidase, and the M-like protein SeM. The proline-rich repeat protein and serine peptidase were confirmed to be immunogenic in horses with strangles, and their sequences were shown to be conserved in global S. equi genomes, in contrast to their diversity in S. equi subsp. zooepidemicus. With the well-characterized S. equi immunogenic protein SeM, this paper identified an immunogenic region outside of the reported critical IgG-binding region. This work provides novel insights to the understanding of the S. equi immunogenic proteins and provides peptide regions that could serve as vaccine candidates against S. equi or as diagnostic markers to specifically identify S. equi infections.
journals.asm.org
And we also characterized the lysis cassette of the Jumboohage phiKZ www.biorxiv.org/content/10.1...
The Lysis Cassette of Jumbophage phiKZ
Pseudomonas jumbophage phiKZ is prominent because of the proteinaceous nuclear structure that is formed during the infection cycle, conferring resistance of the replicating phage DNA to anti-phage res...
biorxiv.org
We recently revised the genome of the paradigm phage SP6 and characterize its lysis genes: www.liebertpub.com/doi/10.1177/...
The Lysis Genes of the Paradigm Phage SP6 | PHAGE
Background: The Salmonella podophage SP6 has been extensively studied as a divergent relative of T7 and as a prototype for therapeutic applications. Two decades ago, it underwent two comprehensive gen...
liebertpub.com
The recipient of the 2025 Thomas Patterson Graduate Student Fellowship is Michael Awuah from @jrrmicro.bsky.social Lab for his work decoding the molecular basis of phage lysis inhibition. We are grateful for the generous support of this award by Drs. Tom Patterson and @superbug-slayer.bsky.social!
We welcome the visit of Dr. Michael Hust and Philip Heine @tu-braunschweig.de @tubsmedbiotech.bsky.social and appreciate their collaboration on ORFeome phage display projects!
@jasonjgill.bsky.social is talking at N=1 Collaborative Seminar TOMORROW 12:30pm EST on "Phage Therapy for Multidrug-Resistant Bacterial Infections: Past and Future" , featuring some of the CPT work. www.n1collaborative.org/post/phage-t...
Phage Therapy for Multidrug-Resistant Bacterial Infections: Past and Future
February 10, 2025 at 12:30 pm US ESTThe session will begin with a presentation from our guest speaker, Dr. Jason Gill, providing an overview of the science and key lessons from bacteriophage developme...
n1collaborative.org
Read our recent paper by @yu-zihao.bsky.social using a fluorescence reporter system that facilitates the visualization of individual copies of phage DNA throughout the phage life cycle, along with the lysis–lysogeny reporters. journals.asm.org/doi/10.1128/...
Fine-tuned spatiotemporal dynamics of DNA replication during phage lambda infection | Journal of Virology
Temperate bacteriophages, such as lambda, incorporate environmental cues including host abundance and nutrient conditions to make optimal decisions between propagation and dormancy. A higher phage-to-...
journals.asm.org
Bonus to our new followers: Our phage database prototype is now online: dbop.phagetech.org. Last updated by @jrrmicro.bsky.social, currently only holds spanin proteins.
dbop
The Center for Phage Technology's Database of Phage
dbop.phagetech.org
Some of our phage protocols including phage DNA extraction, genome sequencing and assembly, and very importantly, genome closure from the assembled contig, are now available online! link.springer.com/protocol/10....
Phage DNA Extraction, Genome Assembly, and Genome Closure
Bacteriophages, or more simply phages, are currently experiencing a renaissance in life science research for their roles in natural microbial communities, their potential use as antimicrobials, and bi...
link.springer.com
Our newest paper on engineering Burkholderia phage is out! pubmed.ncbi.nlm.nih.gov/37943040/
Phage Milagro: a platform for engineering a broad host range virulent phage for Burkholderia - PubMe...
The <i>Burkholderia cepacia</i> complex (Bcc) causes life-threatening respiratory tract infections in persons with cystic fibrosis (CF). In CF patients, end-stage pulmonary disease often requires lung...
pubmed.ncbi.nlm.nih.gov