Detect HiBiT-tagged proteins by flow cytometry. Anti-HiBiT monoclonal antibody fluorophore conjugates offer sensitive signal from XFD dyes (no secondary needed), compatible with fixed & live-cell analysis. Green 488 & Far Red 647. More at: https://ow.ly/kPnN50ZuWMG #FlowCytometry
Promega UK
@promegauk.bsky.social
We're the UK Promega life sciences team, sharing technical tips, our event info, science news, articles and blog posts.
Reminder: free webinar tomorrow, Weds, 2 Sept 2026 at 3.00 p.m. (BST): Hold and Kill – RIPTAC Assay Strategy. Learn how NanoBRET® measures ternary complex formation for this breakthrough "hold-and-kill" approach in clinical trials. https://ow.ly/FCAG50ZwAel #TargetedProteinDegradation #RIPTAC
Lumie's always ready when our LinkedIn Newsletter drops 👀 Fresh insights and industry updates delivered monthly. Subscribe on LinkedIn to join the community at: https://ow.ly/83aC50ZG5Nc #NewsletterCommunity #LifeSciences
Fc Effector Insight: Integrated workflow from lead generation to lot release. Download the Fc Receptors guide covering Lumit® binding assays, ADCC/ADCP Reporter Assays & HiBiT® Target Cell Killing Bioassays at: https://ow.ly/boyr50Zgb4A #AntibodyTherapeutics #FcReceptors
Free webinar Weds, 2 Sept, 3pm BST: Hold and Kill: An Integrated Assay Strategy for RIPTAC, an Emerging Induced-Proximity Modality Learn how NanoBRET® measures ternary complex formation and #assay strategies for #RIPTAC developability. Co-hosted with ICE Bioscience - https://ow.ly/6Wlj50ZwzYj
Most protein detection kills cells first. HiBiT bioluminescence works differently – 11 amino acid tag gives direct, quantitative readout in live cells. Perfect for protein secretion, degrader-induced loss and receptor trafficking. More at: https://ow.ly/4izp50ZuKXs #CellBiology #DrugDiscovery
Consistent results matter for QC and lot release Promega's Thaw-and-Use cells are validated for potency testing across therapeutic targets: FcγR-targeting antibodies, Checkpoint Inhibitors, and Cytokine-GF Receptors. Batch sizes to 1,000 vials, stringent QC https://ow.ly/J1Ap50ZgaJF #Bioassays
Earlier this year at AACR 2026, Promega presented Lumit® hKi-67 Immunoassay for cell proliferation (Poster #477) Missed it? It's worth catching up with over the summer! The bioluminescent method optimises screening across suspension, adherent and 3D cancer models https://ow.ly/GRPf50ZgjMV #AACR
cAMP is a primary second messenger from GPCR activation. Lumit® cAMP Immunoassay: quantitative, no-wash bioluminescent readout directly from cell lysates. No washing, no transfer steps. Learn more at: https://ow.ly/kmE650ZuKzp #GPCR #CellSignalling
Join us tomorrow at 3.00 p.m. BST: for our free webinar on scaling ocean biodiversity monitoring with environmental DNA, with speakers from the Minderoo OceanOmics Centre at the University of Western Australia and Promega. Register at: https://ow.ly/S7Ok50ZhsnI #eDNA #MarineConservation
In manufacturing, reproducibility is everything. Yet cell thawing and expansion introduce major variability. Promega's assay-ready Thaw-and-Use cells arrive ready for immediate use, supporting faster, more consistent workflows from day one. https://ow.ly/FhMz50Zgapp #Bioassays #Manufacturing
GPCR internalisation controls receptor availability. Nano-Glo® HiBiT Extracellular Detection System quantifies cell-surface proteins in real time using antibody-free HiBiT tagging to measure internalisation and recycling. More at: https://ow.ly/vUuQ50ZuI0O #HiBiT #ReceptorInternalisation
What can a single drop of seawater reveal about our oceans? Join us on 19 August 2026 at 3.00 pm BST for a free webinar with the University of Western Australia, exploring how #eDNA workflows are scaling marine biodiversity monitoring - https://ow.ly/cb7550ZhsKI #MarineConservation
Operator-dependent cell handling causes inconsistent batch performance and complicates tech transfer. Promega's assay-ready Thaw-and-Use cells support process consistency by design, reducing variability across teams and sites. More at: https://ow.ly/KO8450Z4O7Q #Bioassays #ThawAndUse
Advanced in vitro #neurotoxicity testing is critical for drug safety Our new Application Note with NETRI shows LDH-Glo™ Cytotoxicity Assay enables sensitive measurements in 2D & 3D microfluidic systems for non-destructive organoid monitoring Download at: https://ow.ly/VJIO50ZgeQ2 #OrganOnChip
Lumie's practising for a Netflix documentary: "The Emotional Weight of Running Promega UK's Social Channels" 😊 Want more of Lumie's adventures? Follow us on Instagram @promega_uk for behind-the-scenes fun and lab life insights! #BehindTheScenes #TeamLife
Drug development slows when early workflows don't support commercial realities. Promega's assay-ready Thaw-and-Use cells support commercial thinking early, moving faster from development to manufacturing without re-engineering. More at: https://ow.ly/8RN950Z4NBA #Bioassays #ThawAndUse
NanoBiT® PPI measures #protein-protein interactions in live cells in real time. GloMax® Galaxy imaging visualises where & how interactions unfold within cells. Example: CX3CR1 recruitment shifts from membrane to intracellular puncta during internalisation - https://ow.ly/8ks850ZuEWQ #NanoBiT
Does your compound engage its GPCR target in living cells? NanoBRET® Target Engagement measures this directly Fluorescent tracer competes with your compound for NanoLuc-tagged GPCR, giving true cellular IC₅₀. No radioactivity, no membrane prep More at: https://ow.ly/zOXF50Zl3hS #NanoBRET #GPCR
With rising biologic drug development, robust analytical methods are essential #PeptideMapping ensures therapeutic antibody quality Proteases with orthogonal cleavage improve sequence coverage, enhance characterisation, and reduce instrument time What challenges do you face? Let’s discuss!
Reminder: Free webinar tomorrow 4pm BST on Live Bioluminescence Imaging for real-time gene expression in human development. Learn how endogenously tagged bioluminescent reporters work in 2D and 3D stem cell models. Register at: https://ow.ly/zrH450ZjYrx #DevelopmentalBiology
New application note: CellTiter-Glo® 3D integrated with MO:BOT automation transforms 3D culture. Less variability, higher signals, better data from seeding to endpoint assay. Facing reproducibility challenges? This is worth reading. https://ow.ly/vHZj50Zl2z3 #3DCellCulture #LabAutomation
Exploring TDCC as a mechanism of action? Promega's complete workflow: Lumit® assays for cytokines, TDCC #Bioassays for T-cell-driven killing, HiBiT assays for target-cell death Clear data from screening to lot release for immune-oncology therapeutics More at: https://ow.ly/xyAC50Zga3m #TDCC
Exploring ADCP as a mechanism of action? Promega's complete workflow: Lumit® assays for FcγR engagement, ADCP Bioassays for phagocytosis readouts, HiBiT assays for target-cell loss. Clear data from screening to lot release. See our website for more information. #Biologics #ADCP
Free webinar Wed 29 July, 4pm BST: Live Bioluminescence Imaging for real-time gene expression in human development. Learn how endogenously tagged bioluminescent reporters work in 2D & 3D stem cell models. Register: https://ow.ly/Az2j50ZjY5k #DevelopmentalBiology
Daraxonrasib nearly doubles survival in metastatic pancreatic cancer versus chemo, targeting "undruggable" RAS for 40+ years. The mechanism was uncovered using NanoBRET® and NanoBiT® assays showing real-time complex formation in living cells. https://ow.ly/79cI50Zl2T5 #DrugDiscovery #NanoBRET
CellTiter-Glo® 3D Cell Viability Assay product page updated with comparison data, FAQs & protocol guidance. Delivers accurate viability in 30 minutes for spheroids, organoids & matrix-embedded cells across hanging-drop, ULA & Matrigel formats https://ow.ly/JA1F50Zk2Pv #3DCellCulture #CellViability
Promega has tools for Fc-mediated activity: Lumit® immunoassays for Fcγ engagement, ADCC Reporter Bioassays for functional responses, HiBiT assays for target cytolysis. Visit our website for more information. #Biologics #ADCC
Do you know how selective your kinase inhibitor is? New customers: 50% off your first compound with Promega's Kinase Selectivity Profiling Services (code #PROFILE26). Quantitative live-cell data across 192 or 300 full-length kinases. https://ow.ly/h4s350Z4F5P #KinaseProfiling #NanoBRET
Not all proliferation assays measure the same thing. Our guide compares five established approaches plus the new Lumit® Cell Proliferation Assay with a side-by-side comparison to help choose the right method. Read the guide at: https://ow.ly/xebg50Zk2rK #CellProliferation #Bioassays