Putri Prasetyaningrum

@putriningrum.bsky.social

🇮🇩(Indonesian) in 🇩🇪 Interested in protein🤝protein, Light and Gibberellin signalling, plant environmental adaptation, cat🐈‍⬛, music🎸 , history🏛️, anthropology🙇

There is still time to apply for the EMBL International PhD Programme! 📢 Don’t miss this opportunity to receive dedicated mentoring while doing interdisciplinary research. Research groups across EMBL are recruiting now! bit.ly/4x9Edss

Bild

Freddy Igiebor, a master student in the lab, used the tools developed in our lab to perform live and fixed imaging of phyB photobodies. Together with the help of Isabella Graf and Laeschkir Würthner, from the EMBL, he began to investigate the biophysical properties of phyB photobodies.

phyB photobodies can have different properties between light conditions and cell types, and so can form molecular memory by retaining signaling biomolecules. phyB photobodies delay and modify light signalling events, so that the plant can react to light when it needs to, and not when it is forced to

This paper was presented at the ISPP in Switzerland last week, just after the presentation of Meng. Our paper deals on how plant phytochrome B (phyB) photoreceptors, which are crucial environmental sensors of light quality and quantity, can form molecular condensates, called phyB photobodies.

Bild

and has touched the work and lives of many of my colleagues. It’s a surreal experience that he is no longer there as a colleague, and he will be greatly missed. I want to dedicate this paper to his memory, and can only wish his close friends and family all the strength to deal with this sudden loss.

Mark your calendars‼️ The SNP2PROT team hosts the "Plant Genetic Variation meets Protein Architecture" international conference, 21-23 June 2027, at the Leopoldina, Halle (Saale). Join us for interdisciplinary discussions, coffee and networking! Registration opens: 17th October, 2026 ⏰ #SNP2Prot

Bild

It worked (after testing an ungodly number of combinations, see supp.)! Three human-derived modules, PHD3, SLY-VHL, HIFODD, form a fully functional O2-dependent proteolysis system in Arabidopsis. We could degrade reporters, TFs, even fluorescent proteins, all reversibly controlled by oxygen levels.

Bild

New Publication: A secreted citrus protease cleaves an outer membrane protein of the Huanglongbing pathogen (2026) https://www.tsl.ac.uk/publications/166327

PNAS: A secreted citrus protease cleaves an outer membrane protein of the Huanglongbing pathogen (2026)

Plants secrete a variety of proteases as a defense response during infection by microbial pathogens. However, the relationship between their catalytic activities and antimicrobial functions remains largely unknown. Particularly, few biologically relevant substrates of these proteases have been identified. Huanglongbing (HLB) has been a major threat to the citrus industry worldwide. The HLB-associated bacterium, "Candidatus Liberibacter asiaticus" (Las), was previously shown to deploy an inhibitor of papain-like cysteine proteases (PLCPs) to promote disease in citrus. In this study, we identified an outer membrane protein (OMP) of Las, LasOMP1, as a substrate of the citrus PLCP CsRD21a. LasOMP1 is one of the most highly expressed genes in Las. CsRD21a cleaves LasOMP1 and produces cleaved peptide products, which could be detected in vitro and in HLB-diseased citrus plants. We found that CsRD21a targets the N-terminal portion of LasOMP1, potentially at an extracellular loop region. Importantly, transgenic sweet orange overexpressing CsRD21a showed reduced Las populations and improved plant growth, highlighting that engineering this protease is a promising strategy to enhance HLB resistance in citrus. Together, our work reveals a pathogen-derived substrate of plant PLCPs and suggests bacterial OMPs may be direct targets of plant defense.

doi.org

And here are this year’s winners of the Reinhold-von-Sengbusch prize for best Poster are: - Gali Araguirang - University Hamburg - Sophie Farkas - University Freiburg - Nazanin Mahouram Mashhadi - University Berlin #MBP2026

Bild