Stomatal movement in Arabidopsis is driven by guard cell-localized and copper-insensitive CSD1 splice variant

Copper (Cu) is an essential micronutrient whose bioavailability is strongly affected by soil physicochemical properties. During evolution, plants have developed mechanisms to flexibly adjust their met...

biorxiv.org

Stress-induced MAPK (SIMK)-dependent organization of microtubules in alfalfa - Molecular Horticulture

Mitogen-activated protein kinases (MAPKs) modulate the organization of the plant cytoskeleton. The spatial organization of microtubules is critical for cell division, polarity, shape control, and elongation during plant growth and morphogenesis. Here, we analyzed the impact of the stress-induced MAPK (SIMK) abundance on the tubulin cytoskeleton in different plant organs and tissues of an important legume crop species, alfalfa (Medicago sativa L.). For this purpose, we have established unique transgenic double lines with genetically manipulated SIMK, possessing microtubular molecular fluorescent marker, the tag RED FLUORESCENT PROTEIN-TUBULIN ALPHA 6 (tagRFP-TUA6). We show that lower or higher SIMK abundance can change cell division planes (CDPs) and phragmoplast orientations in roots. In addition, the transgenic line with downregulated SIMK shows disordered and disorganized microtubules, and a reduced degree of microtubule bundling mainly in leaves and stems. This may be linked to smaller habitus, shorter stems, and smaller leaves. Obtained results indicate that the genetic manipulation of SIMK abundance has effect on microtubule organization and plant development in alfalfa. This study also paves the way for testing anti-microtubular drugs on alfalfa and for biotechnological use of the newly-developed lines.

doi.org

Killers or Protectors: Contrasting Roles of Alfalfa SIMKK and PRKK in Nicotiana benthamiana Cell Death

Mitogen-activated protein kinases (MAPKs) play important roles in immune signalling pathways following pathogen attack or elicitor treatment. A key pl…

sciencedirect.com

2026 Salt and Water Stress in Plants Conference GRC

The 2026 Gordon Research Conference on Salt and Water Stress in Plants will be held in Les Diablerets, Vaud (fr) Switzerland. Apply today to reserve your spot.

grc.org

Linking Superoxide Production and Scavenging in Plant Development

This review examines phytohormone-dependent regulation of NADPH oxidases and superoxide dismutases, controlling superoxide signaling in plant development. Superoxide production and detoxification are...

onlinelibrary.wiley.com

When stress drives you to kidnap: RACK1A sequesters FSD1 into stress granules during salt stress

Describing plants as sessile organisms may sound like a scientific revelation, but it simply means they have nowhere to go. However, it serves as a fitting

academic.oup.com

RACK1A interacts and colocalizes with FSD1 in stress granules to regulate salt stress response in Arabidopsis

A scaffolding protein accumulates in stress granules during salt stress to recruit an antioxidant enzyme, thereby modulating its activity and improving see

doi.org

Linking Superoxide Production and Scavenging in Plant Development

This review examines phytohormone-dependent regulation of NADPH oxidases and superoxide dismutases, controlling superoxide signaling in plant development. Superoxide production and detoxification are...

onlinelibrary.wiley.com

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academic.oup.com

Protein and genetic interactions between RACK1A and FSD1 modulate plant development and stress granule-dependent response to salt in Arabidopsis

The generation of reactive oxygen species (ROS) and their regulation by antioxidant enzymes such as IRON SUPEROXIDE DISMUTASE 1 (FSD1) are critical for managing plant responses to salt stress. However...

biorxiv.org