Friends in the de novo binder protein design space -- if you've designed binders against cell surface proteins and then used them as flow cytometry reagents, I'd love to chat and compare notes!

Two pieces of fun news: 1. We've launched a new website for our lab: www.brysonlab.org 2. With some new funding, we are recruiting for new postdocs and graduate students. The projects build upon our previous studies of antigen presentation and phagosome biology and go into new exciting dimensions!

The Bryson Lab | Vaccine Development & Research at MIT

Developing effective accessible vaccines to end tuberculosis worldwide. Combining immunoengineering, molecular microbiology & systems biology at MIT.

brysonlab.org

The @ragoninstitute.bsky.social is launching a new faculty search for a computational immunologist! Applications are open now and reviewed on a rolling basis. Please share broadly with your networks! #immunosky www.nature.com/naturecareer...

Computational Immunologist - Boston, Massachusetts (US) job with Ragon Institute of MGH, MIT, & Harvard | 12849577

The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard in Cambridge MA, together with the Departmen

nature.com

Happy to share our newest preprint on antigen presentation of Mtb-derived peptides on MHC-I: www.biorxiv.org/cgi/content/... This is a follow up to our previous paper where we applied immunopeptidomics to understand MHC-I presentation by Mtb-infected cells: elifesciences.org/articles/84070

Excited to share our lab's newest preprint (strap in if you're excited about metabolism, species differences, or Mtb gene expression): www.biorxiv.org/content/10.1...

Systems analysis reveals alternate metabolic states adopted by Mycobacterium tuberculosis across species

Mycobacterium tuberculosis (Mtb) persists within macrophages, yet how different host species shape bacterial state remains poorly understood. Here, we directly compared the intracellular transcriptome...

biorxiv.org

Excited to share our newest manuscript on antigen discovery and vaccine design for tuberculosis lead by Owen Leddy (everyone hiring new faculty in a few years, remember this name!) www.science.org/doi/10.1126/... #TBsky

Immunopeptidomics can inform the design of mRNA vaccines for the delivery of Mycobacterium tuberculosis MHC class II antigens

MHC class II immunopeptidomics in human phagocytes infected with Mycobacterium tuberculosis enables vaccine immunogen design and optimization.

science.org

Congrats to the Carpenter Lab on this exciting study! I might now sound like a broken record, but check the abstract!!! Let’s go type 7 secretion system substrates! #tuberculosis #tbsky

Journal of Experimental Medicine@jem.org · 11mo ago

Stetsenko et al. show that TCR clonotypes from individuals latently infected w/ M. #tuberculosis demonstrate dominant but not exclusive recognition of infected #macrophages, specificity for type VII secretion system substrates, & expression of canonical effector programs rupress.org/jem/article/...

Happy to see more data pointing to an important role for type 7 secretion substrates in TB protective immunity

Cell - a Cell Press journal@cp-cell.bsky.social · 11mo ago

Now online! Mining the CD4 antigen repertoire for next-generation tuberculosis vaccines

New video summary 🎥 from Leddy et al. @thebrysonlab.bsky.social (rupress.org/jem/article/... ) on a new workflow called PathMHC that helps find pathogen-derived antigens presented on MHCs by infected cells that could be targets for T cell priming vaccines:

Targeting infection-specific peptides in immunopeptidomics studies for vaccine target discovery

Original paper: https://rupress.org/jem/article/222/10/e20250444/278135/Targeting-infection-specific-peptides-in Video summary by Owen Leddy (Massachusetts Institute of Technology) Description: One…

youtu.be

Newest paper from the lab out today (doi.org/10.1084/jem....)! Here, we describe PathMHC, a novel MS-based approach to identify rare pathogen-derived peptides on MHC-I and MHC-II. We apply this to a range of pathogens Mtb, SARS-CoV-2, and Listeria.

Targeting infection-specific peptides in immunopeptidomics studies for vaccine target discovery

MS-based immunopeptidomics can facilitate vaccine antigen discovery, but self-MHC peptides greatly outnumber pathogen-derived MHC peptides, presenting a ch

doi.org

Anyone have experience with IV mRNA-LNP vaccination in animals? I am specifically interested in LNP formulations and what the preferred formulation for IV is.

Pictured: glass of champagne I treated myself to at DCA upon learning the lab has been tenured! Grateful to my lab family, my extended science family, and my family for the amazing support and love offered on the journey so far. Much more work left to do but taking this moment to say thank you!

Does anyone know of a CRO that produces MVA-based vaccines?

Do any of my #tbsky friends have interests in T cell responses in people with evidence of prior Mtb infection? If so, would you have any interest in looking at T cell responses to Mtb-derived peptides that we have detected by mass spectrometry? LMK 🙏🏽

First post here! To inaugurate this account, I'd like to share my recent work, now published in JACS. Intercepting a Mycobacterial Biosynthetic Pathway with Covalent Labeling | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/...

Intercepting a Mycobacterial Biosynthetic Pathway with Covalent Labeling

The mycobacterial cell envelope plays both infectious and protective roles. Understanding its structure is crucial for unlocking the molecular basis underlying these functions. Studying glycans, the p...

pubs.acs.org

Does anyone have a favorite protocol for in vitro priming of human T cells from PBMCs? #immunosky

So glad to see this out! I learned a lot in this project about how to think creatively about customizing our approach to using language models for immune receptors

Rohit Singh@rohitsingh8080.bsky.social · 2y ago

A bit over 3 years in the making, with first preprint out ~2 years ago, AbMAP has been a labor of love. Joint work with Chiho Im, an amazing MIT undergrad and now a fantastic Stanford grad student, and with Bonnie Berger and @thebrysonlab.bsky.social and @samsl.io and Lena Erlach and ...

Immunopeptidomics (impep) friends, have you ever done impep to ID peptides after soluble protein delivery to phagocytes to look at peptides generated by XP?

Before I write my own analysis script, does anyone in #tbsky have a tool for pathway analysis for Mtb genes?

A lovely day to celebrate the amazing people I work with every day

What is your favorite cytosolic pathogen that (a) infects macrophages and (b) is genetically tractable? Looking for someone who’d like to team up on a small but impactful project (we would do the heavy lifting!)

My whole scientific identity over the last 96 hours has been species differences in immunity. Would love to connect with others on this topic!

Anyone on here love cathepsins or other lysosomal proteases? Would love to pick your brain for 15 minutes!

Anyone want to discuss T cell vaccines? I’ve been in a rabbit hole lately and would love to discuss with others!

I made a Starter Pack for the Myco & TB community. Hope to find more good friends here on Bluesky to keep in touch about mycobacterial infections. Please join and repost! go.bsky.app/3nwpcVV

Looking forward to spending more time on here! Tuberculosis, macrophages, antigen presentation, and systems biology folks — say hey!

#Immunology friends, what are your favorite fix/perm conditions for imaging to access antigens in the phagosomal lumen?

Looking forward to building community on here! Just saying hello for now! Bioengineer at MIT and the Ragon Institute with an interest in tuberculosis, innate immunity, and computational biology! #HiSciSky #HiImmunoSky