Want better DIA #proteomics coverage at the same scan rate with almost zero effort? Get the variable and/or RT-segmented window mz's by uploading your precursor rt-mz table to this free & easy webapp! unitsaq.shinyapps.io/diavariablew... Idea to webapp in 2 days with #ClaudeCode!

Has anyone tried 10 mM AmAc:AmFo (~7:3 molar) as the additive for #LC-MS #lipidomics? Based on Cajka et al, seems like it would be a good compromise for fatty acids vs hydroxysterols to enable consistent RTs between polarities and fast polarity switching doi.org/10.3390/ijms...

Awesome #massspec teaching video for ion trapping--tangible/visible trapping in action: youtu.be link

Electrodynamic Ion Traps - The LinearTrap

YouTube video by Kenneth Libbrecht

youtu.be

I sometimes use Waters PrepMass column calculator for backpressures, volumes, flows, loads, and translating between column dimensions but it doesn't scale down to nanoflow. So I cloned and modified with LLM in a few shots: unitsaq.shinyapps.io/columncalcul... Original: www.waters.com/webassets/ot...

Nice work, demonstrating determination of omega positions in lipid acyls using only RT prediction! www.nature.com/articles/s41...

Computationally unmasking each fatty acyl C=C position in complex lipids by routine LC-MS/MS lipidomics - Nature Communications

Physiologically relevant omega-positions of double bonds in fatty acyls in complex lipids can now only be identified with specialized instrumentation. Here, the authors present a computational approac...

nature.com

#TeamMassSpec Bench Tip: get a 5-kg scale with subgram accuracy for making >200 mL solutions. Faster and more accurate than a graduated cylinder. 1) Measure out solute mass 2) Calculate mass of solvent wrt solution and solvent density (in a reusable spreadsheet) 3) Add solvent mass, done!

Distilled rule of thumb for optimal Protein:Trypsin ratio vs Total Protein concentration in bottom-up #proteomics: Optimal protein:trypsin ≈ 0.1 * protein concentration (ng/uL) Aligns well with standard guidelines e.g. 50:1 at ~500 ng/uL Data from Table 5 in www.sciencedirect.com/science/arti...

Distilled rule of thumb for optimal Cosine cutoffs at various n matching MS2 fragments in #metabolomics ID: Cosine > 0.90 - 0.05*MMP Aligns well with ~80% at 3 matched fragments I learned before. From Fig4a here www.nature.com/articles/s41...

HILIC retention time stability solved?! www.sciencedirect.com/science/arti...

Solving the retention time repeatability problem of hydrophilic interaction liquid chromatography

Hydrophilic interaction (liquid) chromatography (HILIC) has become the first choice LC mode for the separation of hydrophilic analytes. Numerous studi…

sciencedirect.com