Viroscope
@viroscope.bsky.social
Scientific Visualization (PhD) | Viroscope | Revealing the cinematic universe inside your cells | High-End Microscopy & Storytelling
How do you design a multiplex IF experiment with zero bleed-through? Joe McKellar PhD (@viroscope.bsky.social) breaks it down in Part 6 of Ask the Expert. 🟡 KIF5B (21632-1-AP) 🔵 IAV nucleoprotein 🟣 DNA Imaged on @zeiss-microscopy.bsky.social LSM880 Airyscan. #AskTheExpert #Immunofluorescence
Cosmic horror at the sub-cellular scale 🌠🐙 Mutant mouse Mx1 (green) forming tentacle-like structures crawling through a genomic portal (pink; DNA). Like Cthulhu breaching the barriers between realms... Years later, these geometries remain unexplained. 🔬: Joe McKellar
Good antibody or great antibody: what's the difference, and can you see it in an image? Joe McKellar, PhD, @viroscope.bsky.social breaks it down in Part 5 of Ask the Expert. 🟡 CYFIP1/2 (16011-1-AP) 🔵 DNA Imaged on @zeiss-microscopy.bsky.social LSM880 Airyscan #AskTheExpert
The hidden geometry of the cytoskeleton 📐✨ Swipe to see the weird and wonderful world of Septin geometries in this giant human cell: 🟠Septin 7 🔵Actin 🟣DNA 🔬Joe McKellar
Check out the latest part of our Ask the Expert video series with @proteintech.bsky.social, where we talk about some common imaging questions ✨🔬
Proteintech@proteintech.bsky.social · 3w agoSuper-resolution or confocal? How do you choose? 🔬 Joe McKellar PhD (@viroscope.bsky.social) answers in Part 4 of Ask the Expert x Proteintech. 🟡 CYFIP1/2 (16011-1-AP): https://bit.ly/4yd24cf 🔵 DNA Imaged on @zeiss-microscopy.bsky.social LSM880 Airyscan #AskTheExpert #Immunofluorescence
🔵 ⚪ 🔴 A fitting cellular tricolour: blue microtubule highways, white DNA cores, and red membranes. Endlessly grateful to France for the possibilities it has opened up for @viroscope.bsky.social. Bonne fête du 14 Juillet à tous ! #14Juillet #BastilleDay #FranceScience #Microscopy #SciArt
Your experiment "didn't work." But did it? Part 3 of Ask the Expert with @viroscope.bsky.social x Proteintech: negative result vs protocol failure, and how to tell them apart. 🟡 CYFIP1/2 (16011-1-AP) 🔵 DNA Imaged on @zeiss-microscopy.bsky.social LSM880 Airyscan. #AskTheExpert #Immunofluorescence
Painting the cellular cosmos🎨🔬 In science, the boundary between experiment and art can dissolve. Cells become a canvas in total darkness where fluorophores are paint and lasers are our brush. 🟩 DNA 🟧 Actin 🟦 Microtubules 🔬: Joe McKellar
Why does IF signal vary between cells in the same image? Spoiler: it's often not your protocol. Ask the Expert with @_Viroscope, answering the questions every IF researcher has. 🟢 MYC-tag (16286-1-AP): https://bit.ly/4gHF0vT 🟣 IAV nucleoprotein 🟡 DNA #Immunofluorescence
Transport through the microcosmos ✨ In a living cell, a standstill is an alien concept. Molecular machines travel tirelessly through the internal geography. 🟧 Microtubules: Structural hyperloops. 🟪 Vesicles: Cargo pods racing around the grid with incredible precision. 🔬: Joe McKellar
The inner cellular cosmos ✨ Mapping cells to bring an infrastructure that operates in the dark to the light. ◻️ Nucleus: Quiet gravitational center 🟦 Actin: Radiant structural cables 🟧 Septin: Reinforcing scaffolding 🟪 Membranes: Glowing cytoplasmic network Follow for more SciArt! 🔬: Joe McKellar
10h into active viral pathogenesis. Lung cells infected with VSV, showing vesicle trafficking under siege. 🩷 GFP: Replicating virus 💙 Rab11: Endosome network Clear cytopathic distress: extensive membrane blebbing & abnormal nuclear shapes. 🔬 @viroscope.bsky.social. 1 slice, 1 img/sec.
SARS-CoV-2 replicating inside primary Human Airway Epithelia 🟩 Actin: Highlight brush-like cilia and cell-cell junctions 🟪 dsRNA: Replicating virus This image made the cover of Journal of Virology for April 2021.
Check out the final part in the series of #InTheLabWithProteintech !
Proteintech@proteintech.bsky.social · 2mo agoThe final image in our #InTheLabWithProteintech series with Dr Joe McKellar, a 4-colour map of the neuronal cytoskeleton, before the viruses were ever introduced. 🟡 Actin 🔵 Vimentin 🟢 Tubulin 🩷 DNA Read series: https://bit.ly/4e3ehqR Imaged with ZEISS LSM980 + Airyscan 2
#FluorescenceFriday A Maximum Projection of three U2OS cells capturing two different steps of cell division. Sister cells are split but tethered by a midbody remnant. 💛 Actin: Spiky/explosive 💙 DNA: Varing conformations 🖤 Microtubules: Dark depth Separation takes time.
Inner Space: A Cellular Cosmos Two human fibroblasts mimicking cosmic solar bodies. 💛 Actin: Radiating star filaments 🩵 Tubulin: Plasma highways ❤️ DNA: Crimson core We are a universe ourselves. At Viroscope Imaging, we make data breathtaking. Let's elevate your R&D! 🔬 #FluorescenceFriday #SciArt
Septins: the oft forgotten 4th component of the cytoskeleton. This shot of the basal layer reveals SEPT7 (Gold) and SEPT8 (Cyan) in all their structural glory. Check out the blog post and the @proteintech.bsky.social antibody below! ⏬⏬
Proteintech@proteintech.bsky.social · 3mo agoPart two of #InTheLabWithProteintech with Dr Joe McKellar, with septins this time, the “fourth component” of the cytoskeleton, imaged at the basal layer of A549 cells. 🟡 Gold: SEPT7 🔵 Cyan: SEPT8 Read more: https://bit.ly/4d5OE9y @viroscope.bsky.social #InTheLabWithProteintech
The art of the waiting game. Rodent deltavirus (RDeV) is a satellite virus. It doesn't kill, but moves in like a quiet tenant, replicating while waiting for a partner in crime, a helper virus, to provide its transport. 🟨 Actin 🟦 Delta Antigen ⬜ DNA 🔬: Imaged by me.
Building a cellular village 🔬🏘️ You've seen a Tasmanian Devil, but not like this! This super-res cluster of fibroblasts shows one cell building a new unit for the community. 💛 Actin 🩷 DNA 🩵 Tubulin The cyan mitotic spindle is ready for the heavy lifting! Fav mitosis stage? ⬇️
#InTheLabWithProteintech! Go check out the blog post behind the image, and how @proteintech.bsky.social antibodies helped me in my work on viral restriction : bit.ly/4242DX8
Scientist Stories: How Dr Joe McKellar uses Proteintech antibodies in Confocal Microscopy to Study Viral Infections
Discover how Dr Joe McKellar uses Proteintech antibodies in confocal microscopy to study viral infections.
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Proteintech@proteintech.bsky.social · 3mo agoWe're back with our #InTheLabWithProteintech series, with Dr Joe McKellar, a CiteAb Image of the Year winner. A549 cells, 24h into an influenza A infection. 🟡 Gold: YBX1 (hijacked by the virus to replicate) 🔵 Blue: viral nucleoprotein ⚫ Grey: DNA https://bit.ly/4242DX8
My image, "Cellular Cosmic Body," has received an Honorable Mention in the Evident Image of the Year Award. Highlighting radiating Actin and delicate Septin filaments Want your research to have a similar treatment? DM me to collaborate! #SciArt #Microscopy #EvidentIOTY
The high-speed logistics of a lung cancer cell. Watch GFP-Rab25 (Gold) move cargo through the internal recycling pathway of A549 cells. Static maps are great, but time-lapse shows the real traffic. Want your science/tech to be highlighted? DM me at Viroscope!
Sometimes, the data can wait✨ A COS-7 cell reminding us that biology is art. 🩵 Tubulin: Cyan sunburst 💛 Actin: Yellow lace 🩷 DNA Happy #FluorescenceFriday! I turn raw data into cinematic visuals at Viroscope. If you want your science/tech to stand out, reach out! #SciArt
The anatomical landscape of a zebrafish tail 🔬🐟 🔵 Nuclei: Hundreds of individuals in a dense tissular landscape. 🟠 Microtubules: Notice the axonal structural repeats! Want your science/tech to be highlighted? Reach out to me ! #SciArt #Zebrafish
If a cyberpunk metropolis was engineered by biology, it’d look like this. 🔵 Actin: neon structural cables 🟣 MX1: localized defense matrix 🟠 DNA: reactor cores Want your science/tech to look like a movie poster? Reach out to me! 🔬 #SciArt #FluorescenceFriday
Tracking the rapid recycling pathways of a living cell. This time-lapse captures an A549 lung cell expressing GFP-Rab11a. These fast-moving puncta are recycling endosomes, tirelessly shuttling sorted cargo back out to the plasma membrane. Check out the tubulations also! #SciArt #LiveCellImaging
Cellular Liquid Gold 🔬 This time-lapse shows an A549 cell over-expressing Sec61B, outlining the Endoplasmic Reticulum. Imaged at 1 frame per 500ms, it reveals just how violently active our cellular infrastructure really is! What is your favorite organelle to image? 👇 #SciArt #FluorescenceFriday
Kudos to @nadinelaguette.bsky.social team and all co-authors on this nice paper where our lab had a modest contribution! @viroscope.bsky.social 🔥
Journal of Experimental Medicine@jem.org · 4mo agoVila, Messaoud-Nacer, Taffoni, @nadinelaguette.bsky.social et al show that DNA-PKcs, a major DNA damage response actor, interacts w/ cyclic dinucleotides through its kinase domain, ensuring CDN-associated signal termination, while CDNs inhibit DNA-PKcs catalytic activity rupress.org/jem/article/...
