Interesting method...

Stirling Churchman@stirlingchurchman.bsky.social · last wk.

RNA-seq tells us how much RNA is present in the cell. But to understand gene regulation, we need to easily measure the synthesis and decay rates driving this abundance. We introduce AIR-seq: analog intrinsic recoding sequencing. (1/6) www.biorxiv.org/content/10.6...

True that.

Meike Ramon@meikeramon.bsky.social · 3mo ago

Science Needs 3rd Spaces We envision scientific third spaces as “collaborative hubs where researchers, artists, engineers, and practitioners co-develop methods, share resources, and pursue projects driven by curiosity". authors.elsevier.com/sd/article/S...

Interesting, dig in

Mukund Thattai@thattai.bsky.social · 3mo ago

The origin story of eukaryotes has more plot twists than a Hollywood blockbuster! 🧵 Read more in my new review: doi.org/10.1146/annu.... Only in the 1960s were the terms "prokaryote" and "eukaryote" formalized! As originally defined, eukaryotes had complex architectures, prokaryotes did not.

Pathogens & membrane trafficking never cease to amaze. Pathogen-induced formation of a nascent organelle derived from mitochondria Lena Pernas, Xianhe Li, Yubai Sun, Jose M Delgado www.biorxiv.org/content/10.6...

Interesting.

Katharina Geißler@kgeissler.bsky.social · 4mo ago

Out now in Nature Communications! We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky www.nature.com/articles/s41...

I think of a talk like an hourglass. Start broad, get narrow, end broad. So I usually try to end with a brief visual summary (I am not a fan of bullet pointed summaries) and then try to place the work back into the big picture if I can. Good luck!

So cool.... A landmark finding Protein-templated synthesis of dinucleotide repeat DNA by an antiphage reverse transcriptase | Science www.science.org/doi/10.1126/...

Protein-templated synthesis of dinucleotide repeat DNA by an antiphage reverse transcriptase

Defense-associated reverse transcriptases (DRTs) are widespread bacterial anti-phage systems that use unconventional mechanisms of polynucleotide synthesis. We show that DRT3, which comprises two dist...

science.org

Oh wow!!

Harmit Singh Malik@harmitmalik.bsky.social · 4mo ago

Female mice grow testes after this single DNA tweak www.nature.com/articles/d41...

Really cool paper. It has been a real struggle to grow archea. Should they now be cultured with other bacteria? Also interesting to see this symbiosis. www.cell.com/current-biol...

Nice 🧵, lovely movies. Now published advanced.onlinelibrary.wiley.com/doi/10.1002/... from the group of @lauraschaedel.bsky.social

Kinesin‐Induced Buckling Reveals the Limits of Microtubule Self‐Repair

This study shows that kinesin-driven buckling induces extensive microtubule lattice damage that often exceeds intrinsic self-repair and leads to filament failure. While curvature, motor motility, and...

advanced.onlinelibrary.wiley.com

Shweta Nandakumar@bucklingmt.bsky.social · 11mo ago

Despite their high rigidity, microtubules (MTs) dynamically bend and buckle in cells. MT-self repair is inherent to their metastable nature, but it remains unclear whether just inherent repair mechanisms are sufficient to maintain MT integrity🧵 (1/9) www.biorxiv.org/content/10.1...

Trainee Uma: ATG18 catalyzes a budding process that could be directly involved in regulating morphology. Thomas Pucadyil, IISER-Pune: EHD1 is an ATP dependent cutter. Dynamin is involved in retromer trafficking & differentially regulated by EHD family members. #OBMT26

Sunando Dutta, IISER-Bhopal introduces 2 roads to metastasis-single cell or collective invasion. Trainee Amrita: HGF/MET facilitates the invadopodia led charge of cell migration thru the ECM. KIF16B drives MET to invadopodia surface delivery #OBMT26

Probes to quantitatively measure membrane fluidity of intracellular organeller membranes. More fluid mitochondrial inner-membranes are more respiratory. Gaurav Singh, Sunil Laxman lab, inStem #OBMT26

Suchismita: -vely charged nanoparticles are intracellularly retained more than +vely charged NP. The intracellular compartment of +ve & -ve NPs varies as do their mobility & directionality. Content/type of NP inside vesicle may organize membrane surface compsition. Nitin Mohan lab,IIT-Kanpur #OBMT26

Coarse grain Martini molecular model of Caveolin-binding shows that Caveolin both induces and senses membrane curvature. Caveolin binding induced curvature shows that cholesterol & PS are found in the neck. Durba Sengupta, NCL #OBMT26

Clathrin heavy chains mutants associated with intellectual disability. Disease CHC mutants over-expressed in Dm ⬇️ ligand uptake on heat stress, no structural or functional defects at the pre-synapse NMJ but alters post-synaptic architecture and impaired learning. Deepa Subramanyam, NCCS #OBMT26

Allosteric activation switches of AP2. Akshay: Levels of Muniscin family members influence clathrin mediated Transferrin endocytosis. Sarvesh: Phosphorylation of AP2 via BMPK2 & AAK1 regulates clathrin-mediated endocytosis Umasankar Perunthottam lab, NCCS-DBT #OBMT26

Deep dive into how fusion is mediated by viral fusogen proteins w/ pre-fusion, extension & post-fusion confirmations. A fusion loop tryptophan that inserts in the membrane & is enough to cause membrane structure disruption. Tirumala Kumar Chowdary, NISER #OBMT26

EVs & lipid droplets both facilitate virus transmission. Immature JEV egress non-lytically via EVs from neuronal cells. Lipid droplets may be used to form multi vesicular bodies and EVs. Sourish Ghosh, IICB-CSIR #OBMT26

Intro to mechanisms by which Mycobacteria subvert phagosome maturation to evade immune response. Mohd Saleem, NISER. Student Debraj: Mycobacteria release EVs spontaneously fuse w/ host plasma membrane & changes membrane tension. #OBMT26

M. smegmatis shows hypoxia induced differential fluidity in the inner (becomes more rigid) and outer membranes. Hypoxia affects the antibiotic induced membrane permeability. Lydia Mathew, Shobhna Kapoor lab, IIT-Bombay. #OBMT26

Success of the pathogen is the reprogramming of the host cell function. Mtb induces lysosome biogenesis & redistributes them in infected cells via an Mtb lipid. Anterograde lysosome motility is enhanced by Mtb lipids. Varadha Sundaramurthy, NCBS-TIFR #OBMT26

Wingless trafficking occurs with its R where dissociation does not occur during the anterograde route. Wg is internalized together with its cargo R & dissociates in acidic endosomes. Varun Choudhury, IISER-Bhopal. Student Aendrila shows role of TRAPP complex in Wg trafficking. #OBMT26

K-leak channels are non-gated, are always open, imp resting membrane potential & the E/I ratio. Help in cognitive resilience in neurodegenerative disease. 2Pore K channels are on mitochondria & modulate mitochondrial membrane potential. Swagata Ghatak, NISER #OBMT26

Synaptic release is customized for speed and the amount of secretion & regulation may matter more for insulin/immune cell cargo. Synaptobrevin makes dimers and only a few molecules are needed for fusion, although 70 molecules are present/vesicle. Debasis Das @tifr.res.in #OBMT26

Mon1 regulates Drosophila growth through insulin signalling and in a RAB7 independent manner on neuronal morphogenesis. Mon1 acts cell non-autonomously to control post-synaptic glutamate receptors at the NMJ. Anuradha Ratnaparkhi, Agharkar Research Institute #OBMT26

Multistep entry of Influenza virus introduced by Indranil Banerjee, IISER-Mohali. Student Kajal Gupta shows pro-viral roles for TRIM proteins in viral entry via endocytosis. WASH & retromer have opposing roles in IAV endocytosis that is modulated by TRIM. #OBMT26

Syntaxin 11 deficiency impaires cell-intrinsic host defence by enhancing Coxiella & Salmonella replication in monocytes & leads to immune disorders. Nisha Singh, Sandhya Ganesan lab, IISER-Trivandrum #OBMT26

Insep/IER3IP1 loss leads to cell division defects & Rab11 vesicle accumulation in Drosophila neuroblasts/human cells. Insep interacts w/ Yip1d1, whose loss leads to cytokinesis failure & Rab11 vesicle accumulation. Sonal Jaiswal, CCMB-CSIR #OBMT26

Vimlesh Kumar IISER-Bhopal introduces the lab. Student Priyabrata: Prefoldin is imp in tracheal luminal physiology & plasticity thru the cytoskeleton. Student Srikanth: Highwire undergoes LLPS & forms condensates in endocytic mutants. #OBMT26