Yao Chen

@yaochen20.bsky.social

Neuroscientist at Washington University in Saint Louis. Lab web site: https://sites.wustl.edu/yaochenlab/

Glad to be part of this collaboration. These chemigenetic FRET-based sensors have impressive dynamic range in both ratiometric intensity and fluorescence lifetime measurements. The red/far-red sensors enable multiplexed imaging with green sensors, and are functional in brain tissue.

Jin Zhang Lab @jinzhanglab.bsky.social · 6mo ago

Led by grad student Xinchang He and in collaboration with @michellefrei17.bsky.social and @yaochen20.bsky.social we developed a new series of chemigenetic FRET-based biosensors by pairing fluorescent protein donors with HaloTag–far-red dyes, providing enhanced dynamic range over FP-based designs.

Cartoon schematic of YHt-AKAR and RHt-AKAR

Our paper simulating fluorescence lifetime in real-life experiments is now a Version of Record @elife.bsky.social! We hope it helps FLIM users with more rigor and innovation. If you are interested in turning our innovation proposals into reality, we welcome motivated people to join our lab. #FLIM

Yao Chen@yaochen20.bsky.social · last yr.

Our revised manuscript in @elife.bsky.social: The promise and peril of comparing fluorescence lifetime in biology revealed by simulations doi.org/10.7554/eLif...

We have an updated version of our manuscript "The promise and peril of comparing fluorescence lifetime in biology revealed by simulations": www.biorxiv.org/content/10.1.... We have included a Python version in addition to the original Matlab package. Hope this is helpful to the community.

The promise and peril of comparing fluorescence lifetime in biology revealed by simulations

Signaling dynamics are crucial in biological systems, and biosensor-based real-time imaging has revolutionized their analysis. Fluorescence lifetime imaging microscopy (FLIM) excels over the widely us...

biorxiv.org

🧠Excited about neuromodulator interactions or the mystery of sleep? Join our lab at Wash U! We're seeking passionate individuals to bridge cellular and systems neuroscience by decoding biochemical signaling dynamics in the brain. tinyurl.com/2bmf2py9 Postdoc and RA positions available. Pls repost.

Opportunities

We welcome you to join our scientific adventure to understand the function of neuromodulators and the function of sleep, and become future bridge builders between cellular and systems neuroscience.…

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