What is everyone using for only albumin depletion with #proteomics nowadays? We had been using an Agilent spin column but it has been discontinued.

Did you write your comment yet on the OMB rules on federally funded science yet? No? Don’t worry, you can do it this weekend. Here are some resources that can help you get started.

www.biorxiv.org/content/10.6... 1/ Proteomics method development often optimizes for IDs. But for cohort biology, IDs only matter if they remain quantitative, precise, and powered. We ask how chromatographic sampling density propagates from peaks → LOQ → discovery. 🧵

If you are interested in how modern proteomics can shed light on rare childhood diseases, check out this new paper: www.frontiersin.org/journals/mol... TMTpro quantitation works really well. Heads up: it is not a short story. There are decades of knowledge behind the interpretation of the results.

Frontiers | The Nodding syndrome cerebrospinal fluid proteome: a lens into neurodevelopmental failure consistent with environmentally triggered MECP2 dysregulation?

IntroductionNodding Syndrome (NS) is a childhood-onset epileptic encephalopathy of unknown etiology, occurring in clustered outbreaks across East Africa. Des...

frontiersin.org

The Python script reported in Mackie et al. 2018 (Angew Chem Int 57:736–7374) for calculating N and Q deamidation rates from proteomic results has now been ported to the R environment. GitHub github.com/tsutatsuta/r... Zenodo doi.org/10.5281/zeno...

When I was a first year grad student I had the opportunity to meet him. He was incredibly kind to me and an influential advocate for the power of high resolution mass spectrometry. He will be missed.

Joshua Sharp@joshuasharp.bsky.social · last yr.

When I was a fresh young graduate student attending my first ever ASMS, my mentor brought Alan Marshall to my poster. He was curious, clever and kind. And he seemed genuinely delighted that I was showing him something new, even if at the time it was a bit half-baked. He will be missed.

Mass Spec xkcd.com/3094/

USHUPO tips. Flying into Portland and staying at the conference hotel? The light rail goes from the airport to a stop right by the hotel. The line is closed for construction currently but is scheduled to be up and running by March 3rd. trimet.org/traintoplane/

As inspiration strikes I will be posting tips for attendees of USHUPO in Portland. #proteomics

USHUPO tips. Vegan attendees will find lots of options. Portland is very vegan friendly. www.pdxmonthly.com/eat-and-drin...

USHUPO tips. Oregon has no sales tax.

USHUPO tips. The fentanyl crisis is hitting Portland hard. Property crime is high in certain areas. Don’t leave items unattended in rental cars. Old Town is particularly rough right now.

USHUPO tips. There are many amazing neighborhoods and areas aside from downtown. I would suggest the following: NE Alberta Arts District SE Hawthorne SE Division Goose Hollow NE Mississippi Ave travelportland.com has good descriptions of these areas.

USHUPO tips. Portland is well known for beer. There are many small breweries that don’t distribute out of the region preferring to focus on local customers. This blogger is the most influential Portland beer writer IMO. www.beervanablog.com/beervana/202...

Portland's Best Breweries — Beervana

Portland is inarguably one of the country’s great beer cities, but which breweries are the jewels in Beervana’s crown? In my annual feature, I offer the ten breweries doing the best work right now, ...

beervanablog.com

USHUPO tips. Eater PDX has decent neighborhood guides with maps. This one is for the area around the hotel. pdx.eater.com/maps/restaur...

USHUPO tips. Voodoo donuts is mostly visited by tourists. Locals in that area tend to go to Delicious Donuts, Mikikio Mochi Donuts or Doe Donuts.

Would there be interest in a local written guide to the area for HUPO in Portland? #proteomics

Good blog post about how to approach the analysis of a two species proteomics experiment. github.com/pwilmart/Hum...