Structural Biology
@actacrystd.iucr.org
Acta Crystallographica Section D: Structural Biology is a leading structural biology journal published by the IUCr. journals.iucr.org/d
A new type of article for Acta F: Protocols The purpose of this new category is to provide to the reader procedures that are sufficiently detailed so other researchers can reproduce the described workflow. Find out more in 'Show me how it's done: introducing Protocols': doi.org/10.1107/S205...
A quantitative framework for assessing the cost and utilization of high-end scientific equipment, with a particular application to cryo-electron microscopes in structural biology #EquipmentCostModelling #QueueingTheory #CryoElectronMicroscopy doi.org/10.1107/S205...
We're celebrating 20 years of Acta Crystallographica Section F!🎉🥳 Join us by contributing to our celebratory collection of articles, which is now open for submissions! Find out more at journals.iucr.org/special_issu...
Structural studies provide a better understanding of the residues involved in substrate binding and catalysis in a GH5_5 cellulase from Thermoascus aurantiacus #Transglycosylation #Cellulase #GH55Family doi.org/10.1107/S205...
The first crystal structure of gasdermin D bound to a rationally designed peptide ligand reveals peptide engagement at a previously underexplored exosite #GasderminD #Exosite #CyclicPeptides doi.org/10.1107/S205...
To freeze or not to freeze: insights into enzyme crystal structures at room temperature #UrocanateReductase #RoomTemperatureCrystallography #ActiveSiteAnionInteractions doi.org/10.1107/S205...
A comprehensive overview of how X-ray crystallography, cryo-electron microscopy, micro-electron diffraction and cryo-electron tomography have advanced drug discovery across diverse therapeutic areas is provided #XRayCrystallography #CryoEM #CryoET doi.org/10.1107/S205...
Reanalysis of the XFEL data provide structural evidence that supports the assignment of O6 as a state-specific ligand in the S3 state of photosystem II #KokCycle #PhotosystemII #OxygenEvolution doi.org/10.1107/S205...
Big PDB Changes Are One Year Away! On July 21, 2027, wwPDB will fully transition to extended PDB IDs for new depositions and the PDB archive will use a new directory structure based on 12-character extended PDB IDs. Visit wwpdb.org for details.
The crystal structure of Mycobacterium smegmatis MmpL3 in complex with the inhibitor UPAR-1109 at 2.15 Å resolution provides unprecedented insights into the binding mode of the inhibitor #MmpL3 #Indolecarboxamides #CrystalStructure doi.org/10.1107/S205...
Two methods are described for visualizing the H atoms in cryoEM maps of macromolecules #ShoulderPeakDecomposition #RightLeftDifferenceProfilePlot #ModelIndependentMapDecomposition doi.org/10.1107/S205...
The effects of different linkers in fusions of the protein crystallization chaperone 1TEL with target proteins on crystallization were investigated #TELSAM #DARPin #ETV6 doi.org/10.1107/S205...
X-ray-induced spectral changes in common crystallization chemicals and mixtures at cryogenic temperature are mapped: spectroscopic changes can be detected at doses as low as 1 kGy #UVVisAbsorptionSpectroscopy #SpecificRadiationDamage #Radiolysis doi.org/10.1107/S205...
The radiolysis of 5-PMUA exhibits a kinetic transition from flux-limited zero-order decay at RT to retarded target-limited first-order decay at 100 K #RadiationDamage #Enzymology #ReactionKinetics doi.org/10.1107/S205...
A radiation-damage study of the protein rubredoxin from Pyrococcus abyssi at resolutions ranging from 0.54 to 0.57 Å is reported @ActaCrystD @IUCr #RadiationDamage #SubAngstromResolution #FeS4ClusterBondLengths doi.org/10.1107/S205...
The connection between beam damage and image resolution in X-ray and electron microscopy is investigated and the characteristics of electron and X-ray radiolysis are compared to obtain insight into the damage process #RadiationDamage #DoseLimitedResolution #Radiolysis doi.org/10.1107/S205...
D52K, S262Y and D52K/S262Y variants of E. coli flavodiiron-type nitric oxide reductase were introduced in a construct lacking the C-terminal rubredoxin domain #FDP #DiIron #Crystallography doi.org/10.1107/S205...
July issue now available: journals.iucr.org/d/issues/202... The cover highlights Scotty, a tool within the Phasertng codebase that leverages Niggli-cell matches to identify similar crystallographic lattices and perform phasing with difference Fourier methods. Read more at doi.org/10.1107/S205...
Latest from our lab in special issue "CryoEM in the Fast Lane of Structural Biology". Review on CISs in #C.difficile & new results on phage contraction & receptor binding @jasonwils.bsky.social @robfagan.bsky.social @molmicrosheffield.bsky.social #SLayers #CryoEM #Phages doi.org/10.1107/S205...
How cryoEM has advanced our understanding of bacteriophages and bacteriocins targeting Clostridioides difficile
Two recent cryogenic electron microscopy (cryoEM) structures provide a unique opportunity to compare a bacteriophage and a bacteriocin targeted at the same host. CryoEM reveals the most detailed view ...
doi.org
A comparison between the use of electrons and X-rays for collecting diffraction data from small protein crystals and imaging data from cells and tissues #XRayCrystallography #ElectronCrystallography #ElectronImaging doi.org/10.1107/S205...
Modulating redox states in heme peroxidases in synchrotron and XFEL experiments #HemeEnzymes #TimeResolvedCrystallography #Peroxidases doi.org/10.1107/S205...
The Alphafuser protein complex prediction pipeline combines experimental interaction data with systematic querying of possible combinations of protein partners in a computationally parsimonious manner @unistra #AlphaFold #Complexes #Multimers doi.org/10.1107/S205...
MiCSPARC leverages automated particle picking and fast 3D refinement times in CryoSPARC to determine the structures of both decorated and undecorated microtubules#CryoEM #Microtubules #HelicalReconstructions doi.org/10.1107/S205...
When the wrong protein wins: hidden contaminants in cryo-EM #CryoElectronMicroscopy #ProteinPurification #EColiContaminants doi.org/10.1107/S205...
Building national capabilities in cryo-EM #Proteins #StructuralBiology #CryoEM doi.org/10.1107/S205...
The three-dimensional structure of TANGO2 provides new insights into its molecular function and helps to resolve the ongoing debate regarding whether it functions as a heme-trafficking protein #TANGO2 #TDD #NtnHydrolase doi.org/10.1107/S205...
Atomic resolution cryo-EM at 200 keV pubmed.ncbi.nlm.nih.gov/42268577/ #cryoEM
The structure of endo-1,4-β-xylanase A from Anaerobacterium chartisolvens is the first from the newly defined glycoside hydrolase family 30 subfamily 12 #GlycosideHydrolaseFamily30 #GH30 #GH3012 doi.org/10.1107/S205...
The first crystal structures of the luminal domain of Fsc1 reveal an elongated, modular architecture composed of five tandem fasciclin domains #Fsc1 #FasciclinDomain #AutophagosomeVacuoleFusion doi.org/10.1107/S205...
