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Chromatin labeling and imaging group

@andimaging.bsky.social

MPI for Multidisciplinary Sciences Fluorescent #probes for live-cell #nanoscopy: #STED, #MINFLUX and #confocal. We do the job, all of you judge if it is useful!

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Chromatin labeling and imaging group@andimaging.bsky.social · 2y ago

We have introduced a new biocompatible sulfonium-based building block for generation of non-cytotoxic covalent fluorescent probes. The proof-of-concept paper is on BioRxiv: https://doi.org/10.1101/2025.01.27.635008 . Great job done by @MarieAuvray4 at @mpi_nat ! Enjoy reading!

Biocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cells

Fluorescent probes enable the visualization of dynamic cellular processes with high precision, particularly when coupled with super-resolution imaging techniques that surpass the diffraction limit. Traditional methods include fluorescent protein fusion (e.g., GFP) or organic fluorophores linked to ligands targeting the protein of interest. However, these approaches often introduce functional disruptions or ligand-associated biological effects. Herein, we address these challenges by developing covalent fluorescent probes for endogenous tubulin, a critical cytoskeletal protein involved in processes such as cell movement, division, and biomolecule trafficking. Using well-known tubulin binder cabazitaxel and cell permeable fluorophore silicon-rhodamine—as a basis, we introduce a novel biocompatible cleavable linker containing a sulfonium center. This allowed the construction of the optimized probe , 6-SiR- o -C9-CTX , demonstrating excellent cell permeability, fluorogenic properties, and the ability to covalently label tubulin across various human cell lines. Importantly, the targeting moiety could be washed out while preserving tubulin staining, ensuring minimal disruption of tubulin function. This labeling technique is compatible with STED nanoscopy in both live and fixed cells, offering a powerful high-resolution imaging tool. ### Competing Interest Statement G.L. is a co-inventor on the patent (EP2748173B1 and US9346957B2, applicant EPFL) describing SiR and its derivatives.

doi.org

Chromatin labeling and imaging group@andimaging.bsky.social · 3y ago

KTU alumnės chemikės kelias: nuo Marijampolės iki laboratorijos Vokietijoje, kurios departamento vadovas – Nobelio premijos laureatas https://ctf.ktu.edu/news/ktu-alumnes-chemikes-kelias-nuo-marijampoles-iki-laboratorijos-vokietijoje-kuriai-vadovauja-nobelio-premijos-laureatas #ktu

KTU alumnės chemikės kelias: nuo Marijampolės iki laboratorijos Vokietijoje, kurios departamento vadovas – Nobelio premijos laureatas

KTU alumnė dabar studijuoja Makso Planko institute Vokietijoje, o jos departamento vadovas – Nobelio premijos laureatas.

ctf.ktu.edu

Chromatin labeling and imaging group@andimaging.bsky.social · 3y ago

All probes have to be used with caution. The improved design stains DNA brighter and is less toxic: https://doi.org/10.1039/C8SC05082A https://x.com/LSAjournal/status/1704457687100981675

Rhodamine–Hoechst positional isomers for highly efficient staining of heterochromatin

Hoechst conjugates to fluorescent dyes are popular DNA stains for live-cell imaging, but the relationship between their structure and performance remains elusive. This study of carboxyrhodamine–Hoechst 33258 conjugates reveals that a minimal change in the attachment point of the dye has dramatic effects on t

doi.org