Brightness demixing: a method for simultaneously detecting different fluorophores in single-molecule localization microscopy based on brightness. @slfort.bsky.social @laurent-le.bsky.social @ismolab.bsky.social @instlangevin.bsky.social www.nature.com/articles/s41...

Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy - Nature Methods

Brightness demixing is a method for simultaneously detecting different fluorophores in single-molecule localization microscopy based on their brightness rather than their spectral properties. As this ...

nature.com

New paper alert! Le et al. introduce brightness demixing, a method that distinguishes fluorophores by their photon flux rather than spectral properties. Congrats! Authors used our #DNAPAINT reagents to image clathrin, tubulin, vimentin, & nuclear pores. 🔗 www.nature.com/articles/s41...

Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy - Nature Methods

Brightness demixing is a method for simultaneously detecting different fluorophores in single-molecule localization microscopy based on their brightness rather than their spectral properties. As this ...

nature.com

Big thanks to Andrea Bucci and @laurent-le.bsky.social for the 13h drive with a microscope in the trunk...and setting it up! First workshop #MifoBio2025 done, challenge won. A #SPADarray in any laser-scanning microscope. @genoainstruments.bsky.social @iitalk.bsky.social @gdrimabio.bsky.social

Time for the second #MifoBio2025 workshop with @laurent-le.bsky.social and Andrea Bucci on building a #fluorescencelifetime #imagescanningmicroscopy system. Thanks @erc.europa.eu, @iitalk.bsky.social and @genoainstruments.bsky.social #SPADarray #BrightEyes_ERC #photonresolvedmicroscopy

🔬Happy to share our new preprint, where brightness is used to identify single molecule emission in 2D/3D, with a single camera ! Part of the PhD work of @laurent-le.bsky.social and Surabhi, great collaboration with @emmanuelfort.bsky.social from @instlangevin.bsky.social @ismolab.bsky.social #SMLM

bioRxivpreprint@biorxivpreprint.bsky.social · last yr.

Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy https://www.biorxiv.org/content/10.1101/2025.03.02.639924v1

And last but not least Laurent Le from the team on part of his PhD work @ismolab.bsky.social