Ultrafast 3D imaging just got faster! Introducing: "Instant dual-view Snouty (iDV-Snouty) light-sheet microscopy" -> Double the data rate of a Snouty microscope by adding a 2nd view! Use the extra view for a variety of imaging modes! Article here: amsikking.github.io/idv-snouty_l...

I’m hiring for a microscopy image analysis role at Calico! www.calicolabs.com/careers/?gh_... We’re looking for someone at ~recent PhD lvl who has previous experience with biological samples and analyzing microscopy datasets. Feel free to reach out with any questions!

Careers - Calico

calicolabs.com

Analyzing organelles is HARD. They're complex, but critical to understanding biology. Current tools are manual, organelle-specific, or can't handle large 3D datasets. With an easy install and a click of a button, Nellie solves ALL these problems! nature.com/articles/s41... 🧵2/N

Nellie: automated organelle segmentation, tracking and hierarchical feature extraction in 2D/3D live-cell microscopy - Nature Methods

Nellie is a comprehensive automated pipeline for studying the structure and intracellular dynamics of diverse organelles that offers accurate segmentation, tracking and feature extraction on both 2D a...

nature.com

Already some cool applications for Nellie: If you're used to MitoGraph data and want some of the same outputs from Nellie, check out Gav's Python code.

From Lefebvre and colleagues, Nellie is a comprehensive automated pipeline for studying the structure and intracellular dynamics of diverse organelles that offers accurate segmentation, tracking and feature extraction on both 2D and 3D data. www.nature.com/articles/s41...

We spend a lot of time thinking about segmenting cells, but organelles represent a similarly important challenge that is tricky due to their diversity in shapes and sizes. Please check out Nellie, a generalist tool for segmenting AND TRACKING organelles!

Austin E. Y. T. Lefebvre@austin-lefebvre.bsky.social · last yr.

Do you LOVE organelles?? Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social! Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy 🧵1/N

Do you LOVE organelles?? Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social! Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy 🧵1/N

Stoked to share TWO preprints on mitochondrial pearling! 🎉 We uncover how spontaneous #mitochondria pearling drives #mtDNA nucleoid distribution (doi.org/10.1101/2024...), and @gavsturm.bsky.social et al. dissect the biophysics behind it (doi.org/10.1101/2024...). Details below! 🔬🧵🧪