Bert De Rybel

@bertderybel.bsky.social

Plant developmental biologist with a key interest in vascular tissues, cell division orientation and single cell omics at VIB/UGent 🇧🇪🇪🇺🌱

Phloem is the highly specialised sugar conducting tissue found in all living vascular plants, and it is today always occurs with xylem, the specialised water conducting tissue. Both of these tissues enabled plants to become larger and more complex, but how did these tissues evolve? 1/

Congratulation @transitionalform.bsky.social @instmolplantsci.bsky.social for publishing the next chapter of your thesis @currentbiology.bsky.social ! Great work showing that the two fundamental vascular tissues - xylem and phloem evolved asynchronously. Read Laura's thread for more details 👇👇

Laura Cooper@transitionalform.bsky.social · last mo.

Our paper on the origin and evolution of phloem, a big part of my PhD work, is out in Current Biology! www.sciencedirect.com/science/arti...

Happy to announce that I have started my own research group at the MPIPZ and that I am looking for a postdoc who would like to investigate Marchantia prothallus development with me! Please spread the word and/or apply if interested :)

Max Planck Institute for Plant Breeding Research@mpipz.bsky.social · 2mo ago

Looking for a #postdoc? 📣We are #hiring📣 Join the Wallner Group @mpipz.bsky.social to work on tissue patterning, polarity & body axes formation with the liverwort Marchantia polymorpha.🌿 Apply now! #plantsci 👉https://jobs.mpipz.mpg.de/jobposting/54b1ba6d403b8a106f2595c1ef1171f8d729bb720?ref=homepage

job description

Not sure which scRNA-seq platform to use for 🪴 plant samples? How well do doublet detection algorithms really work in 🌱? How can I optimise sample prep ? Find out in our benchmark study! Led by @carogro.bsky.social and @thomaseekhout.bsky.social. Open access at EMBO Journal: doi.org/10.1038/s443...

Benchmarking plant single cell RNA-sequencing sample processing strategies - The EMBO Journal

The isolation of single plant cells from complex tissues is prone to selective enrichment and sampling biases, which complicates accurate profiling of the large diversity in cell types. Optimizing methodologies for cell enrichment and single-cell transcriptomics is therefore critical for single-cell studies addressing plant cell heterogeneity. Here, we systematically compared protoplast enrichment technologies (including conventional and image-based flow cytometry, as well as magnetic cell sorting) and single-cell RNA sequencing (scRNA-seq) platforms (10X Genomics Chromium, BD Rhapsody) using Arabidopsis roots. Image-based flow cytometry offered increased precision due to customizable gating strategies, while magnetic sorting provided faster processing and enhanced representation of cell size heterogeneity. Both scRNA-seq platforms captured root cell heterogeneity and yielded reproducible gene expression profiles, but showed platform-associated differences in cell type composition. Notably, single-nucleotide polymorphism analysis of a mixed ecotype sample revealed that, among cells identified as doublets by computational algorithms, two-thirds were likely to have been misclassified. These insights identify key biases in plant cell purification and scRNA-seq workflows and provide practical guidance for improving data quality across plant species.

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🚨 PhD Position in Plant Molecular Biology 🌱 We are looking for a motivated PhD student to join our team to study root protective barriers. If you are interested in confocal microscopy, cell wall biology, and plant development, this project might be for you! Please share! #PlantScience #PlantSciJob

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