Proud to share that another one of my main PhD projects has now been published! The manuscript entitled "Exploring PadR Proteins for Artificial Enzyme Design" is now available online in #ChemBioChem. Keep readin below or check out the full paper here: doi.org/10.1002/cbic... Thread ⬇️

Exploring PadR Proteins for Artificial Enzyme Design

Inspired by the Lactococcal multidrug resistance regulator (LmrR) protein scaffold, new phenolic acid decarboxylase transcriptional regulator (PadR) proteins were identified and evaluated as scaffold...

doi.org

🥳🥳🥳 Our study on engineering enzymes to break C–F bonds is out in @angewandtechemie.bsky.social. Congrats to the brilliant & dedicated @suzannejansen.bsky.social for taking this step toward the remediation of #PFAS & other contaminating organofluorides! onlinelibrary.wiley.com/doi/10.1002/...

Engineering Fluoroacetate Dehalogenase by Growth‐Based Selections on Non‐Natural Organofluorides

We present a high-throughput selection system to engineer fluoroacetate dehalogenases (FAcDs). By challenging E. coli populations that produce diverse FAcD libraries to grow on non-natural organofluo....

onlinelibrary.wiley.com

🚨 preprint 2️⃣ this month: our (purely experimental🧪) venture into #ChemBio We prouldy present: ADD-tagging of proteins (or "ADDing") —a super convenient enzymatic technique to install click chemistry handles on proteins. Led by superstar @wahyuwidodo.bsky.social www.biorxiv.org/content/10.1... A 🧵👇🏽

📢📢📢 PhD Opportunity! Join our research group at RUG! We're seeking a motivated PhD student for a project on biocatalytic cascade processes combining natural and designer enzymes, supported by machine learning. Click below to learn more — and feel free to share! sites.google.com/rug.nl/roelf...

Roelfes group - Open Positions

PhD position on Hybrid biocatalytic cascades aided by Machine learning (1.0 FTE)

sites.google.com

Very proud to share that one of my main PhD projects has been published in @chemicalscience.rsc.org, and that it has been selected as a #ChemSciPicks of the week paper! Keep reading below for more details, or check out the full paper for free online: doi.org/10.1039/D5SC... Thread ⬇️

Chemical Science@chemicalscience.rsc.org · last yr.

Check out this week’s #ChemSciPicks from Gerard Roelfes (@roelfesgroup.bsky.social, @stratinghinst.bsky.social) et al.! ‘Genetically Encoded 3-Aminotyrosine as Catalytic Residue in a Designer Friedel-Crafts Alkylase’ Read it here for free: doi.org/10.1039/D5SC... #ChemSky 🧪

Grateful to be part of this awesome project lead by @brouwerb.bsky.social. Take a look to our preprint about unlocking iminium ion catalysis when using amino-tyrosine as a genetically encoded amino acid! 🤓 #MSCA @roelfesgroup.bsky.social

Bart Brouwer@brouwerb.bsky.social · last yr.

Check out our latest preprint, in which we demonstrate the first example of using noncanonical 3-aminotyrosine (aY) as a catalytic residue for iminium activation in a designer enzyme! Thread ⬇️

Check out our latest preprint, in which we demonstrate the first example of using noncanonical 3-aminotyrosine (aY) as a catalytic residue for iminium activation in a designer enzyme! Thread ⬇️