Over the past 50 years, we have learned much about how transcription factors and other DNA-binding proteins search for their targets, but we know much less about how sequence-specific RNA-binding proteins (RBPs) search for their targets. 🧵⤵️ (1/26)

My lab has developed mSHINE, allowing for both highly multiplexed and portable nucleic acid detection. Very exciting work led by the talented @sijbren-kramer.bsky.social, who is off to do a PhD at Stanford at the end of the month!

Sijbren Kramer@sijbren-kramer.bsky.social · 12mo ago

Multiplexed nucleic acid detection is critical for the surveillance of pathogens and mutations, but existing technologies either require extensive lab equipment or have low multiplexing. We built a portable microfluidic manifold for massively multiplexed CRISPR-based detection. (1/12)

Multiplexed nucleic acid detection is critical for the surveillance of pathogens and mutations, but existing technologies either require extensive lab equipment or have low multiplexing. We built a portable microfluidic manifold for massively multiplexed CRISPR-based detection. (1/12)

A handheld microfluidic manifold for massively multiplexed CRISPR-based nucleic acid detection

Multiplexed methods for nucleic acid detection are immensely challenging to deploy outside of laboratory settings. Conversely, field-deployable methods are limited to low levels of multiplexing. Durin...

biorxiv.org

Our latest paper using SHINE for TB detection directly from sputum just came out in Science Advances! See the thread from co-first author Alex Bell for more details...

Alexandra G Bell@alexandragbell.bsky.social · 12mo ago

Despite antibiotic treatments, tuberculosis (TB) remains an enormous global health burden, in part driven by the difficulties in timely and accurate diagnosis. Can we use CRISPR to improve TB diagnosis to make it faster, simpler, and more accessible? (1/21)