🚨 Today we report cryo-EM structures of the Bxb1 integrase complexed with its attB/attP DNA substrates before, during and after recombination. Using these structures, we engineer Bxb1 mutants with improved recombination efficiencies and altered sequence recognition properties. Check it out! ⬇️

Structure and engineering of the large serine recombinase Bxb1 for gene integration

Soma et al. present cryo-EM structures of the large serine recombinase Bxb1 bound to attP and attB substrates and provide mechanistic insights into attP/attB recognition, synaptic complex formation, a...

cell.com

If you like transposons... If you you love genome editing... Or if you just like random bird animations, we have the paper for you! We (@kedmonds.bsky.social et al) are happy to share our work turning a songbird retrotransposon into a genome editing tool. 🐣 (1/n)

STITCHR, a tool that can make virtually any kind of edit in mammalian genomes including the scarless multi-kilobase scale insertions. Plasmids here www.addgene.org/browse/artic...

Chris Fell@cfell.bsky.social · last yr.

So excited to share our new paper out today in @nature.com. We repurposed R2 retrotransposons to create STITCHR, a tool that can make virtually any kind of edit in mammalian genomes including the scarless multi-kilobase scale insertions 🧵 www.nature.com/articles/s41...

So excited to share our new paper out today in @nature.com. We repurposed R2 retrotransposons to create STITCHR, a tool that can make virtually any kind of edit in mammalian genomes including the scarless multi-kilobase scale insertions 🧵 www.nature.com/articles/s41...

Reprogramming site-specific retrotransposon activity to new DNA sites - Nature

A study of retrotransposon activity repurposes a retroelement called R2Tocc to create a programmable system called STITCHR that enables diverse genome edits including efficient, scarless large pa...

nature.com

🚨 Interested in precise, multi-kilobase scale insertions into the genome? Check out our detailed protocol describing PASTE, out in Nature Protocols @natureportfolio.bsky.social ⬇️ www.nature.com/articles/s41...

Precise kilobase-scale genomic insertions in mammalian cells using PASTE - Nature Protocols

Programmable addition via site-specific targeting elements (PASTE) combines the specificity, efficiency and cargo size benefits of site-specific integrases with the programmability of prime editing fo...

nature.com